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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0248 [new locus tag: SA_RS01460 ]
  • pan locus tag?: SAUPAN001136000
  • symbol: SA0248
  • pan gene symbol?: tarS
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0248 [new locus tag: SA_RS01460 ]
  • symbol: SA0248
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 301718..303439
  • length: 1722
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGAAATTTTCAGTAATAGTTCCAACATACAATTCAGAAAAGTATATAACAGAATTA
    CTTAATAGCCTTGCGAAACAAGATTTTCCGAAAACTGAATTTGAAGTGGTTGTAGTTGAT
    GACTGTTCAACAGATCAAACGTTACAAATAGTTGAAAAGTATCGCAATAAATTGAACTTG
    AAAGTAAGTCAACTCGAAACAAATTCTGGTGGTCCAGGTAAACCTAGAAATGTGGCGTTA
    AAACAAGCAGAAGGTGAATTTGTATTATTTGTGGACTCCGATGACTATATAAACAAAGAG
    ACTTTAAAGGATGCAGCAGCATTTATTGATGAACATCACTCAGATGTCTTATTGATTAAA
    ATGAAAGGTGTTAATGGTCGTGGTGTACCACAATCTATGTTTAAAGAAACAGCACCTGAA
    GTTACTTTGTTAAATTCAAGAATTATCTATACTTTAAGCCCGACTAAAATCTATAGAACA
    GCATTACTAAAAGATAATGACATTTATTTTCCAGAAGAATTAAAGAGTGCAGAAGATCAA
    TTATTTACAATGAAAGCATATTTGAATGCAAATCGAATCAGTGTGTTAAGTGATAAAGCG
    TATTATTATGCTACAAAGCGTGAAGGTGAACATATGAGTAGTGCGTATGTTTCACCTGAA
    GACTTTTACGAAGTCATGAGATTGATTGCTGTAGAAATATTAAATGCAGATTTAGAAGAA
    GCTCATAAAGATCAAATCTTAGCAGAATTTTTAAATCGTCATTTTAGTTTTTCTCGTACG
    AATGGCTTCTCACTTAAAGTTAAACTTGAAGAGCAACCACAATGGATTAATGCTCTAGGA
    GACTTTATACAAGCAGTTCCAGAACGTGTAGATGCATTGGTGATGAGTAAATTACGACCA
    TTGTTGCACTACGCGAGAGCGAAAGATATAGACAACTATAGAACTGTAGAAGAAAGTTAC
    CGTCAAGGTCAATACTACCGTTTTGATATTGTAGATGGTAAATTAAACATTCAATTCAAT
    GAAGGCGAACCATACTTTGAAGGCATTGATATCGCTAAGCCAAAAGTGAAAATGACAGCA
    TTTAAATTTGATAATCATAAAATTGTTACAGAGCTAACGTTAAATGAATTTATGATTGGC
    GAAGGACATTATGATGTCAGACTTAAATTACATTCACGAAACAAGAAGCACACAATGTAT
    GTACCTTTAAGTGTCAATGCGAATAAACAATATCGTTTTAACATTATGTTAGAAGATATT
    AAAGCGTATTTACCTAAAGAAAAAATTTGGGATGTTTTCTTAGAAGTCCAAATAGGTACG
    GAAGTATTTGAAGTGCGTGTTGGTAATCAACGTAATAAATATGCATATACTGCAGAAACA
    AGTGCATTAATTCATTTAAATAATGATTTTTATAGATTAACACCGTATTTCACAAAAGAC
    TTTAATAACATTTCGTTATACTTTACAGCTATTACATTAACGGATTCAATCTCAATGAAG
    TTAAAAGGTAAAAACAAAATCATTTTAACTGGTCTGGATCGTGGTTATGTATTTGAAGAA
    GGTATGGCTAGTGTCGTGCTAAAAGACGACATGATTATGGGAATGTTAAGCCAAACGTCA
    GAAAACGAAGTGGAAATCTTACTTAGTAAAGATATTAAGAAGCGAGACTTCAAAAATATT
    GTTAAGTTAAACACTGCACATATGACTTACTCGCTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1722

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0248 [new locus tag: SA_RS01460 ]
  • symbol: SA0248
  • description: hypothetical protein
  • length: 573
  • theoretical pI: 6.53193
  • theoretical MW: 66256.5
  • GRAVY: -0.371553

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 57.6)
    mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 56.9)
    Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 51)
    poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 48.2)
    and 8 more
    chitooligosaccharide synthase NodC (TIGR04242; EC 2.4.1.-; HMM-score: 44.4)
    glycosyltransferase domain (TIGR04440; EC 2.-.-.-; HMM-score: 40.7)
    colanic acid biosynthesis glycosyltransferase WcaA (TIGR04017; EC 2.4.-.-; HMM-score: 36.7)
    hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 28.1)
    colanic acid biosynthesis glycosyltransferase WcaE (TIGR04009; EC 2.4.-.-; HMM-score: 27.7)
    glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 22.2)
    cellulose synthase catalytic subunit (UDP-forming) (TIGR03030; EC 2.4.1.12; HMM-score: 21.7)
    hopanoid biosynthesis associated glycosyl transferase protein HpnI (TIGR03472; HMM-score: 10.7)
  • TheSEED  :
    • Poly(ribitol-phosphate) beta-N-acetylglucosaminyltransferase (EC 2.4.1.355)
  • ⊞PFAM:
    E-set (CL0159) TarS_C2; TarS C-terminal domain 2 (PF22377; HMM-score: 172.2)
    and 7 more
    TarS_C1; TarS beta-glycosyltransferase C-terminal domain 1 (PF18674; HMM-score: 132.9)
    GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 113.3)
    Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 51.1)
    Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 48.9)
    no clan defined TarS_linker; Glycosyl transferase TarS linker domain (PF22181; HMM-score: 46.4)
    GT-A (CL0110) Glyco_tranf_2_4; Glycosyl transferase family 2 (PF13704; HMM-score: 19.5)
    no clan defined DUF4478; Pyrimidine/purine nucleotide 5'-monophosphate nucleosidases (PF14793; HMM-score: 13.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7965
    • Cytoplasmic Membrane Score: 0.1571
    • Cell wall & surface Score: 0.0169
    • Extracellular Score: 0.0295
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007451
    • TAT(Tat/SPI): 0.000169
    • LIPO(Sec/SPII): 0.001186
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCSTDQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETLKDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRTALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVSPEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEEQPQWINALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHSRNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQRNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKIILTGLDRGYVFEEGMASVVLKDDMIMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTAHMTYSLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Stephanie Brown, Guoqing Xia, Lyly G Luhachack, Jennifer Campbell, Timothy C Meredith, Calvin Chen, Volker Winstel, Cordula Gekeler, Javier E Irazoqui, Andreas Peschel, Suzanne Walker
Methicillin resistance in Staphylococcus aureus requires glycosylated wall teichoic acids.
Proc Natl Acad Sci U S A: 2012, 109(46);18909-14
[PubMed:23027967] [WorldCat.org] [DOI] (I p)

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