PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0020 [new locus tag: NWMN_RS00115 ]
• pan locus tag^?: SAUPAN000035000
• symbol: NWMN_0020
• pan gene symbol^?: walI
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0020 [new locus tag: NWMN_RS00115 ]
• symbol: NWMN_0020
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 28800..29588
• length: 789
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5329926 NCBI
• RefSeq: YP_001331055 NCBI
• BioCyc:
• MicrobesOnline: 3705551 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAACTGGAAACTGACAAAGACACTTTTCATTTTCGTGTTTATTCTTGTCAACATCGTG
  TTAGTATCGATTTATGTTAATAAAGTCAATCGCTCACACATTAATGAAGTCGAGAGTAAC
  AATGAAGTTAATTTTCAGCAAGAAGAAATTAAAGTACCGACTAGTATATTGAATAAATCA
  GTTAAAGGTATAAAATTAGAGCAAATTACAGGGCGATCAAAAGACTTTAGTTCTAAAGCT
  AAAGGCGATTCGGATTTGACCACATCAGATGGTGGAAAATTATTGAATGCGAACATTAGT
  CAATCGGTAAAGGTCAGTGACAATAACTTAAAAGATTTGAAAGATTATGTTAACAAGCGC
  GTATTTAAAGGTGCTGAATATCAATTAAGCGAGATTAGTTCAGATTCTGTAAAATATGAA
  CAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATA
  GAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGAT
  GGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTAT
  AATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTC
  GTGAATGAAACGAATGTTCAATTGTTACAACCAAACTGGGAAATTAAAGTGAAGCATGAC
  GGTAAGGATAAAACGAATACTTACTATGTCGAAGCGACAAATAATAACCCTAAAATTATT
  AATCATTAA
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⊟Protein

⊟General

• locus tag: NWMN_0020 [new locus tag: NWMN_RS00115 ]
• symbol: NWMN_0020
• description: hypothetical protein
• length: 262
• theoretical pI: 9.36924
• theoretical MW: 29931.6
• GRAVY: -0.621756

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  YycI_YycH (CL0285) YycI; YycH protein (PF09648; HMM-score: 175)
  and 2 more
  no clan defined DUF2730; Protein of unknown function (DUF2730) (PF10805; HMM-score: 14.4)
  ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.448612
  • TAT(Tat/SPI): 0.000871
  • LIPO(Sec/SPII): 0.040542
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151220232 NCBI
• RefSeq: YP_001331055 NCBI
• UniProt: A0A0H3K5H4 UniProt

⊟Protein sequence

• MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVNFQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQSVKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNIEDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYYSVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: vicR > vicK > NWMN_0019 > NWMN_0020

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Olivier Poupel, Mati Moyat, Julie Groizeleau, Luísa C S Antunes, Simonetta Gribaldo, Tarek Msadek, Sarah Dubrac
Transcriptional Analysis and Subcellular Protein Localization Reveal Specific Features of the Essential WalKR System in Staphylococcus aureus.
PLoS One: 2016, 11(3);e0151449
[PubMed:26999783] [WorldCat.org] [DOI] (I e)