PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0019 [new locus tag: NWMN_RS00110 ]
• pan locus tag^?: SAUPAN000034000
• symbol: NWMN_0019
• pan gene symbol^?: walH
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0019 [new locus tag: NWMN_RS00110 ]
• symbol: NWMN_0019
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 27438..28799
• length: 1362
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332593 NCBI
• RefSeq: YP_001331054 NCBI
• BioCyc:
• MicrobesOnline: 3705550 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  GTGAAGTCATTGAAGACGGTGATTGGGATGAATAATAAGGAGCATATTAAATCTGTCATT
  TTAGCACTACTCGTCTTGATGAGTGTCGTATTGACATATATGGTATGGAACTTTTCTCCT
  GATATTGCAAATGTCGACAATACAGATAGTAAGAAGAGTGAAACGAAACCTTTAACGACA
  CCTATGACAGCCAAAATGGATACAACTATTACGCCATTTCAGATTATTCATTCGAAAAAT
  GATCATCCAGAAGGAACGATTGCGACGGTATCTAATGTGAATAAACTGACGAAACCTTTG
  AAAAATAAAGAAGTGAAGTCCGTGGAACATGTTCGTCGTGATCATAACTTGATGATTCCT
  GATTTGAACAGTGATTTTATATTATTCGATTTTACGTATGATTTACCGTTATCAACATAT
  CTTGGTCAAGTACTGAACATGAATGCGAAAGTACCAAATCATTTCAATTTCAATCGTTTG
  GTCATAGATCATGATGCTGATGATAATATCGTGCTTTATGCTATAAGCAAAGATCGCCAC
  GATTACGTAAAATTAACAACTACAACGAAAAATGATCATTTTTTAGATGCATTAGCAGCA
  GTGAAAAAAGATATGCAACCATACACAGATATCATCACAAACAAAGATACAATTGATCGT
  ACGACGCATGTTTTTGCACCAAGTAAACCTGAAAAGTTAAAAACATATCGCATGGTATTT
  AACACGATTAGTGTTGAGAAAATGAATGCTATACTATTTGACGATTCAACCATCGTTCGT
  AGTTCAAAGAGTGGTGTTACAACCTACAACAATAATACAGGTGTCGCAAACTATAACGAT
  AAAAATGAAAAATATCATTATAAAAACCTGTCCGAAGATGAAGCGAGTTCCAGCAAAATG
  GAAGAAACGATTCCAGGAACCTTTGATTTTATTAATGGTCATGGTGGTTTCTTAAACGAA
  GACTTTAGATTGTTTAGTACGAATAATCAGTCAGGCGAGTTAACATATCAACGTTTCCTT
  AATGGTTATCCAACGTTTAATAAAGAAGGTTCTAATCAAATTCAAGTCACTTGGGGTGAA
  AAAGGCGTCTTTGACTATCGTCGTTCGTTATTACGCACCGACGTTGTTTTAAATAGTGAG
  GATAATAAATCGTTGCCGAAATTAGAGTCTGTACGTTCAAGCTTAGCGAACAATAGTGAT
  ATTAATTTTGAAAAAGTAACAAACATCGCTATCGGTTACGAAATGCAGGATAATTCAGAT
  CATAATCACATTGAAGTGCAGATTAACAGTGAACTCGTACCGCGTTGGTATGTAGAATAT
  GATGGCGAATGGTATGTTTATAACGATGGGAGGCTTGAATAA
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⊟Protein

⊟General

• locus tag: NWMN_0019 [new locus tag: NWMN_RS00110 ]
• symbol: NWMN_0019
• description: hypothetical protein
• length: 453
• theoretical pI: 6.03146
• theoretical MW: 51882.9
• GRAVY: -0.563355

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein C (TIGR01713; HMM-score: 16.6)
  Cellular processes Cellular processes Sporulation and germination sporulation protein YunB (TIGR02832; HMM-score: 15.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  YycI_YycH (CL0285) YycH; YycH protein (PF07435; HMM-score: 451.4)
  and 1 more
  no clan defined Gly_acyl_tr_N; Aralkyl acyl-CoA:amino acid N-acyltransferase (PF06021; HMM-score: 10.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 2
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.236353
  • TAT(Tat/SPI): 0.000626
  • LIPO(Sec/SPII): 0.005152
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151220231 NCBI
• RefSeq: YP_001331054 NCBI
• UniProt: A0A0H3KD70 UniProt

⊟Protein sequence

• MKSLKTVIGMNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKSETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHVRRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDNIVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAPSKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKYHYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGYPTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDINFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: vicR > vicK > NWMN_0019 > NWMN_0020

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Olivier Poupel, Mati Moyat, Julie Groizeleau, Luísa C S Antunes, Simonetta Gribaldo, Tarek Msadek, Sarah Dubrac
Transcriptional Analysis and Subcellular Protein Localization Reveal Specific Features of the Essential WalKR System in Staphylococcus aureus.
PLoS One: 2016, 11(3);e0151449
[PubMed:26999783] [WorldCat.org] [DOI] (I e)