PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0022 [new locus tag: SAUSA300_RS00115 ]
• pan locus tag^?: SAUPAN000034000
• symbol: SAUSA300_0022
• pan gene symbol^?: walH
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0022 [new locus tag: SAUSA300_RS00115 ]
• symbol: SAUSA300_0022
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 27536..28819
• length: 1284
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914326 NCBI
• RefSeq: YP_492742 NCBI
• BioCyc: see SAUSA300_RS00115
• MicrobesOnline: 1291537 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAGTGTCGTATTGACATATATGGTATGGAACTTTTCTCCTGATATTGCAAATGTCGAC
  AATACAGATAGTAAGAAGAGTGAAACGAAACCTTTAACGACACCTATGACAGCCAAAATG
  GATACAACTATTACGCCATTTCAGATTATTCATTCGAAAAATGATCATCCAGAAGGAACG
  ATTGCGACGGTATCTAATGTGAATAAACTGACGAAACCTTTGAAAAATAAAGAAGTGAAG
  TCCGTGGAACATGTTCGTCGTGATCATAACTTGATGATTCCTGATTTGAACAGTGATTTT
  ATATTATTCGATTTTACGTATGATTTACCGTTATCAACATATCTTGGTCAAGTACTGAAC
  ATGAATGCGAAAGTACCAAATCATTTCAATTTCAATCGTTTGGTCATAGATCATGATGCT
  GATGATAATATCGTGCTTTATGCTATAAGCAAAGATCGCCACGATTACGTAAAATTAACA
  ACTACAACGAAAAATGATCATTTTTTAGATGCATTAGCAGCAGTGAAAAAAGATATGCAA
  CCATACACAGATATCATCACAAACAAAGATACAATTGATCGTACGACGCATGTTTTTGCA
  CCAAGTAAACCTGAAAAGTTAAAAACATATCGCATGGTATTTAACACGATTAGTGTTGAG
  AAAATGAATGCTATACTATTTGACGATTCAACCATCGTTCGTAGTTCAAAGAGTGGTGTT
  ACAACCTACAACAATAATACAGGTGTCGCAAACTATAACGATAAAAATGAAAAATATCAT
  TATAAAAACCTGTCCGAAGATGAAGCGAGTTCCAGCAAAATGGAAGAAACGATTCCAGGA
  ACCTTTGATTTTATTAATGGTCATGGTGGTTTCTTAAACGAAGACTTTAGATTGTTTAGT
  ACGAATAATCAGTCAGGCGAGTTAACATATCAACGTTTCCTTAATGGTTATCCAACGTTT
  AATAAAGAAGGTTCTAATCAAATTCAAGTCACTTGGGGTGAAAAAGGCGTCTTTGACTAT
  CGTCGTTCGTTATTACGCACCGACGTTGTTTTAAATAGTGAGGATAATAAATCGTTGCCG
  AAATTAGAGTCTGTACGTTCAAGCTTAGCGAACAATAGTGATATTAATTTTGAAAAAGTA
  ACAAACATCGCTATCGGTTACGAAATGCAGGATAATTCAGATCATAATCACATTGAAGTG
  CAGATTAACAGTGAACTCGTACCGCGTTGGTATGTAGAATATGATGGCGAATGGTATGTT
  TATAACGATGGGAGGCTTGAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0022 [new locus tag: SAUSA300_RS00115 ]
• symbol: SAUSA300_0022
• description: hypothetical protein
• length: 427
• theoretical pI: 5.44435
• theoretical MW: 49007.3
• GRAVY: -0.641452

⊟Function

• TIGRFAM:
• TheSEED  :
  • FIG011501: YycH protein
  Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles CPO analysis  FIG011501: YycH protein
• PFAM:
  YycI_YycH (CL0285) YycH; YycH protein (PF07435; HMM-score: 427.4)
  and 1 more
  no clan defined Gly_acyl_tr_N; Aralkyl acyl-CoA:amino acid N-acyltransferase (PF06021; HMM-score: 11)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.047176
  • TAT(Tat/SPI): 0.001419
  • LIPO(Sec/SPII): 0.011136
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162050 NCBI
• RefSeq: YP_492742 NCBI
• UniProt: A0A0H2XID8 UniProt

⊟Protein sequence

• MSVVLTYMVWNFSPDIANVDNTDSKKSETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHVRRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDNIVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAPSKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKYHYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGYPTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDINFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0020 > SAUSA300_0021 > SAUSA300_0022 > SAUSA300_0023

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Olivier Poupel, Mati Moyat, Julie Groizeleau, Luísa C S Antunes, Simonetta Gribaldo, Tarek Msadek, Sarah Dubrac
Transcriptional Analysis and Subcellular Protein Localization Reveal Specific Features of the Essential WalKR System in Staphylococcus aureus.
PLoS One: 2016, 11(3);e0151449
[PubMed:26999783] [WorldCat.org] [DOI] (I e)