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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002165
  • pan locus tag?: SAUPAN005592000
  • symbol: lacA
  • pan gene symbol?: lacA
  • synonym:
  • product: galactose-6-phosphate isomerase subunit LacA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002165
  • symbol: lacA
  • product: galactose-6-phosphate isomerase subunit LacA
  • replicon: chromosome
  • strand: -
  • coordinates: 2183386..2183814
  • length: 429
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGCGATTATTATTGGTTCAGATGAAGCTGGCAAACGATTAAAAGAAGTCATCAAATCA
    TACTTATTAGACAATAAATATGATGTTGTTGACGTAACAGAAGGACAGGAAGTTGACTTT
    GTTGATGCAACTTTGGCTGTAGCAAAAGATGTTCAAAGTCAAGAAGGTAACTTAGGTATT
    GTTATCGATGCATTTGGTGCAGGTAGCTTTATGGTTGCTACAAAAATCAAAGGCATGATT
    GCTGCTGAAGTATCAGATGAACGCTCTGGATATATGACTAGAGGCCATAACAATTCAAGA
    ATGATCACTATGGGATCTGAAATTGTTGGAGACACATTAGCGAAAAATGTCGTTAAAGGT
    TTTGTTGAAGGTAAATACGATGGTGGTAGACACCAAATCCGCGTAGATATGCTTAATAAA
    ATGTGCTAA
    60
    120
    180
    240
    300
    360
    420
    429

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002165
  • symbol: LacA
  • description: galactose-6-phosphate isomerase subunit LacA
  • length: 142
  • theoretical pI: 4.96862
  • theoretical MW: 15394.5
  • GRAVY: -0.105634

⊟Function[edit | edit source]

  • reaction:
    EC 5.3.1.26?  ExPASy
    Galactose-6-phosphate isomerase D-galactose 6-phosphate = D-tagatose 6-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides galactose-6-phosphate isomerase, LacA subunit (TIGR01118; EC 5.3.1.26; HMM-score: 284.6)
    and 4 more
    sugar-phosphate isomerase, RpiB/LacA/LacB family (TIGR00689; EC 5.3.1.-; HMM-score: 110.3)
    Metabolism Energy metabolism Pentose phosphate pathway ribose 5-phosphate isomerase B (TIGR01120; EC 5.3.1.6; HMM-score: 102.4)
    ribose 5-phosphate isomerase (TIGR02133; EC 5.3.1.6; HMM-score: 79.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides galactose-6-phosphate isomerase, LacB subunit (TIGR01119; EC 5.3.1.26; HMM-score: 40.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined LacAB_rpiB; Ribose/Galactose Isomerase (PF02502; HMM-score: 132.5)
    and 2 more
    DUF7376; Family of unknown function (DUF7376) (PF24091; HMM-score: 13.7)
    DUF5760; Family of unknown function (DUF5760) (PF19064; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9432
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0567
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002486
    • TAT(Tat/SPI): 0.000093
    • LIPO(Sec/SPII): 0.000229
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDATLAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMITMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LacR* (repression) regulon
    LacR*(TF)important in Lactose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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