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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002160
  • pan locus tag?: SAUPAN005586000
  • symbol: JSNZ_002160
  • pan gene symbol?: lacE
  • synonym:
  • product: lactose-specific PTS transporter subunit EIIC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002160
  • symbol: JSNZ_002160
  • product: lactose-specific PTS transporter subunit EIIC
  • replicon: chromosome
  • strand: -
  • coordinates: 2178875..2180587
  • length: 1713
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGCAAAAATTGATCGCACAAATTGAAAAAGGGAAGCCATTTTTCGAGAAATTATCA
    CGTAACATATATTTAAGAGCCATCCGTGATGGATTTATTTCTGCAATGCCTGTCATCTTA
    TTCTCAAGTATCTTTCTATTAATTGCATACGTGCCAAATATATTTGGTTTTAAATGGGAT
    AAAGGCATGGAAGCCATTTTAATGAAACCATATAACTATACGATGGGGCTTGTAGCATTT
    TTAGTAGCAGGAACGACAGCGAAATCTTTAACAGATTCATTTAACCGTAAGCTAGAAAGT
    ACAAATCAAATTAACTTTATTTCTACAATGCTAGCAGCGATGTGTGGATTCTTGTTCTTA
    GCTTCTGATCCAGCCAAAGATGGTGGATTCTTAAGTGCATTTATGGGAACTAAAGGTTTA
    TTAACAGCATTTTTATCAGCATTTGTAACTGTTATTGTTTATAACTTCTGTGTGAAGCGC
    AATATTACAATTAAAATGCCGAAAGAAGTACCGCCGAATATTTCACAAGTATTTAAGGAC
    TTAATTCCATTTTCAGCGGTAATCATCATTCTTTATGCATTAGATTTAGTCATTCGCAAC
    AGCTTTAAATCAAATGTAGCGGAAGGTATTTTAAAATTATTCGAACCATTATTTACAGCA
    GCAGATGGATGGATTGGTGTCACAATTATCTTTGGTGCCTTTGCATTATTCTGGTTTGTA
    GGAATTCATGGTCCATCAATAGTAGAGCCAGCAATTGCAGCCATTACATATGCGAATATC
    GAAGCGAACTTCAAGTTGCTTCAAGCTGGAGAACACGCAGATAAAATTATTACATCAGGT
    ACACAAATGTTTATCGTTACTTTTGGAGGTACGGGTGCAACATTGGTCGTCCCGTTCATG
    TTTATGTGGATGACGAAATCTAAACGAAACAAAGCGATTGGACGTGCATCAGTTGTACCA
    ACATTCTTTGGTGTAAACGAACCAATTTTATTTGGTGCACCACTAGTATTGAATCCTGTG
    TTCTTTATTCCATTTGTATTAGCACCAATTGTTAACGTATGGATTTTCAAACTTTTCGTT
    GAAGTGTTAGGAATGAATAGTTTTAGTGTGAATTTACCTTGGACAACACCAGGTCCATTG
    GGCATTATCATGGGTACAGGTTTTGGTTTATGGTCATTCGTACTAGCTATTACTTTGATT
    GTTGTAGATATTATTATTTACTACCCATTCCTAAAAGTTTATGATAGTGAAATTCTTGAT
    GAAGAAGAAGGACGTAAAGAAAGTAATTCAGATTTAAAAGAAAAAGTTGCAGCAAACTTT
    GATACGAAAAAAGCTGATTCAATTTTAGCGGCAAGTGGTGTATCAGAAGATGCAGCTAAA
    GCTTCAAATATCACAGAACAAACAAATGTCTTAGTACTTTGTGCCGGTGGTGGTACAAGT
    GGATTATTAGCCAATGCACTTAATAAAGCAGCTGAAGAATATCATGTGCCAGTGAAAGCG
    GCAGCTGGCGGATATGGCGCACATATGGACATTATGAAAGAGTACCAATTAATCATTTTA
    GCGCCACAAGTTGCGTCTAACTATGAAGATATTAAGCAAGATACAGATAGATTAGGTATT
    AAATTAGCGAAGACTCAAGGTGCTGAATATATCAAATTGACACGAGATGGCCAAGCAGCC
    CTTGATTTCGTACAAAAACAATTTGAAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1713

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002160
  • symbol: JSNZ_002160
  • description: lactose-specific PTS transporter subunit EIIC
  • length: 570
  • theoretical pI: 8.01173
  • theoretical MW: 62445.9
  • GRAVY: 0.441754

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.207?  ExPASy
    Protein-N(pi)-phosphohistidine--lactose phosphotransferase [Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2)
  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 771.4)
    Signal transduction Signal transduction PTS PTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 771.4)
    and 8 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 267.3)
    Signal transduction Signal transduction PTS PTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 267.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 267.3)
    Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 267.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.2)
    Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.2)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 33.9)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 26.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 277.3)
    and 2 more
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 39.1)
    PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9992
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0008
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001663
    • TAT(Tat/SPI): 0.000132
    • LIPO(Sec/SPII): 0.00121
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKRNITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSEDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQKQFEN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LacR* (repression) regulon
    LacR*(TF)important in Lactose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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