PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002160
• pan locus tag^?: SAUPAN005586000
• symbol: JSNZ_002160
• pan gene symbol^?: lacE
• synonym:
• product: lactose-specific PTS transporter subunit EIIC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002160
• symbol: JSNZ_002160
• product: lactose-specific PTS transporter subunit EIIC
• replicon: chromosome
• strand: -
• coordinates: 2178875..2180587
• length: 1713
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  
  ATGATGCAAAAATTGATCGCACAAATTGAAAAAGGGAAGCCATTTTTCGAGAAATTATCA
  CGTAACATATATTTAAGAGCCATCCGTGATGGATTTATTTCTGCAATGCCTGTCATCTTA
  TTCTCAAGTATCTTTCTATTAATTGCATACGTGCCAAATATATTTGGTTTTAAATGGGAT
  AAAGGCATGGAAGCCATTTTAATGAAACCATATAACTATACGATGGGGCTTGTAGCATTT
  TTAGTAGCAGGAACGACAGCGAAATCTTTAACAGATTCATTTAACCGTAAGCTAGAAAGT
  ACAAATCAAATTAACTTTATTTCTACAATGCTAGCAGCGATGTGTGGATTCTTGTTCTTA
  GCTTCTGATCCAGCCAAAGATGGTGGATTCTTAAGTGCATTTATGGGAACTAAAGGTTTA
  TTAACAGCATTTTTATCAGCATTTGTAACTGTTATTGTTTATAACTTCTGTGTGAAGCGC
  AATATTACAATTAAAATGCCGAAAGAAGTACCGCCGAATATTTCACAAGTATTTAAGGAC
  TTAATTCCATTTTCAGCGGTAATCATCATTCTTTATGCATTAGATTTAGTCATTCGCAAC
  AGCTTTAAATCAAATGTAGCGGAAGGTATTTTAAAATTATTCGAACCATTATTTACAGCA
  GCAGATGGATGGATTGGTGTCACAATTATCTTTGGTGCCTTTGCATTATTCTGGTTTGTA
  GGAATTCATGGTCCATCAATAGTAGAGCCAGCAATTGCAGCCATTACATATGCGAATATC
  GAAGCGAACTTCAAGTTGCTTCAAGCTGGAGAACACGCAGATAAAATTATTACATCAGGT
  ACACAAATGTTTATCGTTACTTTTGGAGGTACGGGTGCAACATTGGTCGTCCCGTTCATG
  TTTATGTGGATGACGAAATCTAAACGAAACAAAGCGATTGGACGTGCATCAGTTGTACCA
  ACATTCTTTGGTGTAAACGAACCAATTTTATTTGGTGCACCACTAGTATTGAATCCTGTG
  TTCTTTATTCCATTTGTATTAGCACCAATTGTTAACGTATGGATTTTCAAACTTTTCGTT
  GAAGTGTTAGGAATGAATAGTTTTAGTGTGAATTTACCTTGGACAACACCAGGTCCATTG
  GGCATTATCATGGGTACAGGTTTTGGTTTATGGTCATTCGTACTAGCTATTACTTTGATT
  GTTGTAGATATTATTATTTACTACCCATTCCTAAAAGTTTATGATAGTGAAATTCTTGAT
  GAAGAAGAAGGACGTAAAGAAAGTAATTCAGATTTAAAAGAAAAAGTTGCAGCAAACTTT
  GATACGAAAAAAGCTGATTCAATTTTAGCGGCAAGTGGTGTATCAGAAGATGCAGCTAAA
  GCTTCAAATATCACAGAACAAACAAATGTCTTAGTACTTTGTGCCGGTGGTGGTACAAGT
  GGATTATTAGCCAATGCACTTAATAAAGCAGCTGAAGAATATCATGTGCCAGTGAAAGCG
  GCAGCTGGCGGATATGGCGCACATATGGACATTATGAAAGAGTACCAATTAATCATTTTA
  GCGCCACAAGTTGCGTCTAACTATGAAGATATTAAGCAAGATACAGATAGATTAGGTATT
  AAATTAGCGAAGACTCAAGGTGCTGAATATATCAAATTGACACGAGATGGCCAAGCAGCC
  CTTGATTTCGTACAAAAACAATTTGAAAATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
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  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1713
  

⊟Protein

⊟General

• locus tag: JSNZ_002160
• symbol: JSNZ_002160
• description: lactose-specific PTS transporter subunit EIIC
• length: 570
• theoretical pI: 8.01173
• theoretical MW: 62445.9
• GRAVY: 0.441754

⊟Function

• reaction:
  EC 2.7.1.207?  ExPASy
  Protein-N(pi)-phosphohistidine--lactose phosphotransferase [Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2)
• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 771.4)
  Signal transduction Signal transduction PTS PTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 771.4)
  and 8 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 267.3)
  Signal transduction Signal transduction PTS PTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 267.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 267.3)
  Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 267.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.2)
  Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.2)
  PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 33.9)
  PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 26.4)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 277.3)
  and 2 more
  Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 39.1)
  PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 8
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9992
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0008
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001663
  • TAT(Tat/SPI): 0.000132
  • LIPO(Sec/SPII): 0.00121
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKRNITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSEDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQKQFEN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications