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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002119
  • pan locus tag?: SAUPAN005500000
  • symbol: JSNZ_002119
  • pan gene symbol?: mtlD
  • synonym:
  • product: mannitol-1-phosphate 5-dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002119
  • symbol: JSNZ_002119
  • product: mannitol-1-phosphate 5-dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2131510..2132616
  • length: 1107
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAAGCAGTTCACTTTGGTGCTGGTAACATAGGTCGTGGTTTCATTGGTTATATTCTT
    GCAGACAACAATGTTAAAGTAACATTTGCAGACGTCAATGAAGAAATCATTAATGCTTTA
    GCTCATGATCATCAATACGATGTTATTTTAGCTGATGAGTCTAAAACAACGACGCGCGTG
    AATAATGTTGATGCAATTAATTCAATGCAACCTTCTGAAGCGTTGAAACAAGCAATTCTA
    GAAGCTGATATTATTACAACAGCTGTTGGTGTTAACATACTACCTATTATTGCTAAATCT
    TTTGCGCCTTTCTTAAAAGAAAAAACAAACCATGTTAATATTGTTGCTTGTGAGAATGCT
    ATTATGGCAACTGATACATTGAAAAAAGCAGTACTTGATATTACTGGCCCTCTTGGTAAC
    AATATTCATTTTGCTAACTCAGCAGTTGATAGAATTGTACCATTACAAAAGAATGAAAAT
    ATATTAGACGTTATGGTTGAGCCATTTTACGAATGGGTTGTTGAAAAAGATGCATGGTAT
    GGTCCAGAACTAAACCATATTAAATATGTTGATGATTTAACACCATATATTGAGCGTAAA
    TTATTAACTGTGAATACAGGACATGCATATTTAGCGTATGCTGGTAAATTTGCAGGTAAA
    GCTACCGTATTAGACGCTGTAAAAGATAGTTCAATTGAAGCTGGCTTACGCCGTGTTTTA
    GCTGAAACAAGTCAATATATTACTAATGAATTTGATTTTACTGAAGCGGAACAAGCTGGT
    TATGTTGAAAAAATAATAGACCGTTTCAACAATTCTTATTTATCTGATGAAGTGACACGT
    GTCGGACGAGGTACATTACGTAAAATTGGCCCTAAAGATAGAATTATAAAACCATTAACA
    TATCTTTATAATAAAGATTTAGAACGCACTGGTTTATTAAATACAGCTGCATTGTTATTG
    AAGTATGATGATACAGCAGACCAAGAAACTGTTGAGAAAAATAATTACATTAAAGAACAC
    GGTTTAAAAGCGTTTTTAAGTGAATATGCTAAAGTTGACGATGGCTTAGCCGATGAAATA
    ATTGAAGCGTACAATTCACTTTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1107

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002119
  • symbol: JSNZ_002119
  • description: mannitol-1-phosphate 5-dehydrogenase
  • length: 368
  • theoretical pI: 4.76262
  • theoretical MW: 40936.1
  • GRAVY: -0.178533

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.17?  ExPASy
    Mannitol-1-phosphate 5-dehydrogenase D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH
  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Mannitol_dh; Mannitol dehydrogenase Rossmann domain (PF01232; HMM-score: 169.3)
    and 8 more
    6PGD_C (CL0106) Mannitol_dh_C; Mannitol dehydrogenase C-terminal domain (PF08125; HMM-score: 116.7)
    NADP_Rossmann (CL0063) 3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 21.1)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 20.4)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 19.2)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 16.1)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 13.3)
    no clan defined Pam3_gp32; Phage Pam3 gp32-like protein (PF23992; HMM-score: 12.5)
    NADP_Rossmann (CL0063) Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9966
    • Cytoplasmic Membrane Score: 0.0006
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0024
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.017057
    • TAT(Tat/SPI): 0.000312
    • LIPO(Sec/SPII): 0.001306
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDHQYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFAPFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNENILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFAGKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSDEVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKNNYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: MtlR* (activation) regulon, SigB (activation) regulon
    MtlR*(TF)important in Mannitol utilization; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026) 
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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