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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2060 [new locus tag: NWMN_RS11895 ]
  • pan locus tag?: SAUPAN005500000
  • symbol: mtlD
  • pan gene symbol?: mtlD
  • synonym:
  • product: mannitol-1-phosphate 5-dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2060 [new locus tag: NWMN_RS11895 ]
  • symbol: mtlD
  • product: mannitol-1-phosphate 5-dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2281347..2282453
  • length: 1107
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAAGCAGTTCACTTTGGTGCTGGTAACATAGGTCGTGGTTTCATTGGTTATATTCTT
    GCAGACAACAATGTTAAAGTAACATTTGCAGACGTCAATGAAGAAATCATTAATGCTTTA
    GCTCATGATCATCAATACGATGTTATTTTAGCTGATGAGTCTAAAACAACGACGCGCGTG
    AATAATGTTGATGCAATTAATTCAATGCAACCTTCTGAAGCGTTGAAACAAGCAATTCTA
    GAAGCTGATATTATTACAACAGCTGTTGGTGTTAACATACTACCTATTATTGCTAAATCT
    TTTGCGCCTTTCTTAAAAGAAAAAACAAACCATGTTAATATTGTTGCTTGTGAGAATGCT
    ATTATGGCAACTGATACATTGAAAAAAGCAGTACTTGATATTACTGGCCCTCTTGGTAAC
    AATATTCATTTTGCTAACTCAGCAGTTGATAGAATTGTACCATTACAAAAGAATGAAAAT
    ATATTAGACGTTATGGTTGAGCCATTTTACGAATGGGTTGTTGAAAAAGATGCATGGTAT
    GGTCCAGAACTAAACCATATTAAATATGTTGATGATTTAACACCATATATTGAGCGTAAA
    TTATTAACTGTGAATACAGGACATGCATATTTAGCGTATGCTGGTAAATTTGCAGGTAAA
    GCTACAGTTTTAGATGCAGTTGAAGATAGTTCAATTGAAGCTGGCTTACGCCGTGTTTTA
    GCTGAAACTAGTCAATATATTACTAATGAATTTGATTTTACTGAAGCGGAACAAGCTGCT
    TATGTTGAAAAAATAATAGATCGTTTCAACAATTCTTATTTATCTGATGAAGTAACACGT
    GTCGGACGAGGTACATTACGTAAAATTGGCCCTAAAGATAGAATTATAAAACCATTAACA
    TATCTTTATAATAAAGATTTAGAACGCACTGGTTTATTAAATACAGCTGCATTGTTATTG
    AAGTATGATGATACAGCAGACCAAGAAACTGTTGAGAAAAATAATTACATTAAAGAACAC
    GGTTTAAAAGCGTTTTTAAGTGAATATGCTAAAGTTGACGATGGCTTAGCCGATGAAATA
    ATTGAAGCGTACAATTCACTTTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1107

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2060 [new locus tag: NWMN_RS11895 ]
  • symbol: MtlD
  • description: mannitol-1-phosphate 5-dehydrogenase
  • length: 368
  • theoretical pI: 4.66047
  • theoretical MW: 40951
  • GRAVY: -0.171467

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.17?  ExPASy
    Mannitol-1-phosphate 5-dehydrogenase D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH
  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    6PGD_C (CL0106) Mannitol_dh_C; Mannitol dehydrogenase C-terminal domain (PF08125; HMM-score: 144.5)
    and 6 more
    NADP_Rossmann (CL0063) Mannitol_dh; Mannitol dehydrogenase Rossmann domain (PF01232; HMM-score: 71.2)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 20.8)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 18.6)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 18.4)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 16.1)
    Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.017057
    • TAT(Tat/SPI): 0.000312
    • LIPO(Sec/SPII): 0.001306
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDHQYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFAPFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNENILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFAGKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSDEVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKNNYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: MtlR* (activation) regulon, SigB (activation) regulon
    MtlR*(TF)important in Mannitol utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1] [2]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  2. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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