From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001857
  • pan locus tag?: SAUPAN004794000
  • symbol: JSNZ_001857
  • pan gene symbol?: glnP
  • synonym:
  • product: ABC transporter permease subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001857
  • symbol: JSNZ_001857
  • product: ABC transporter permease subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 1894696..1896153
  • length: 1458
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAGTGTTTAATTAGGTTTATATTAGTATTAGGGTTGTTAATTAGCAGTGCTATGGTG
    TATATCAATCCCACAGCACATGCTGAACAAGATCAAACATGGGAGAAGATTAAAGAACGC
    GGTGAACTTAGAGTGGGTCTTTCTGCAGATTATGCACCAATGGAATTTGAGCATACAGTT
    AATGGTAAGACTGAGTATGCAGGTGTAGATATTGATTTAGCTAAAAAAATTGCGAAAGAT
    AATAATTTAAAATTAAAAATCGTCAATATGTCATTTGATAGTTTGTTAGGAGCTCTTAAA
    ACTGGAAAAATTGATATTATTATTTCCGGAATGACTTCAACGCCTGAACGTAAGAAGCAA
    GTTGATTTTTCAGATTCATATATGATGACTAAAAATATCATGCTTGTAAAGAAAGATAAA
    GTTAATGAATATAAAGATATCAAAGACTTTAATAATAAAAAAGTAGGGGCACAAAAGGGA
    ACTGAACAAGAAAAAATCGCTCAAACCGAAATTGAAAATGCATCTATTACTTCATTAAGC
    CGTTTACCAGACGTTATATTAGCATTGAAAAGTGGAAAGGTTGAAGGTGCTGTAGTTGAA
    AAACCTGTAGCAGAAGCATATTTAAAACAAAATCCTAAATTAGGAATTTCCAATGTGAAA
    TTTAATGAAGAAGAAAAAGATACAGTGATAGCTGTGCCAAAAGATTCACCAAAATTATTG
    TCACAAATTAATAAAACGATTAAAGAGGTTAAAGATAAAGGATTAATCGATAAATATATG
    ACTAATGCTGCAAATGCGATGAATGATGACAGTGGTTTTATTTCTAAGTATGGTAGTTTT
    TTCTTGAAAGGAATTAAGATTACAATATTAATTTCACTTATCGGTGTTGCATTAGGTTCT
    ATTTTAGGTGCATTCGTTGCGTTAATGAAATTAAGTAAAATAAAAATTATTTCATGGATT
    GCTTCTATCTATATCGAAATATTAAGAGGAACACCAATGTTAGTTCAAGTATTTATCGTA
    TTCTTTGGTATAACTGCTGCATTAGGACTAGACATTTCAGCTCTTGTTTGTGGAACAATT
    GCATTAGTTATTAATTCCTCAGCTTACATTGCTGAAATTATTCGTGCAGGTATAAATGCT
    GTTGATAAAGGACAAATGGAAGCCGCACGTAGTCTAGGTTTGAATTATAGACAAACGATG
    AAAAGTGTAATTATGCCACAAGCAATTAAAAATATTTTACCAGCTTTAGGTAATGAATTT
    GTCACTTTAATTAAAGAATCATCTATTGTGTCAACAATTGGAGTTGGCGAAATTATGTTT
    AATGCACAAGTGGTTCAAGGTATTTCATTTGACCCATTCACACCATTACTAGTGGCAGCA
    GCATTATACTTTGTCTTAACATTTGTACTTACACGCATTATGAACATGATTGAAGGGAGA
    TTGAATGCCAGTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1458

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001857
  • symbol: JSNZ_001857
  • description: ABC transporter permease subunit
  • length: 485
  • theoretical pI: 9.56041
  • theoretical MW: 53122.2
  • GRAVY: 0.210928

⊟Function[edit | edit source]

  • TIGRFAM:
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 158.7)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 153.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines lysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 136.6)
    and 12 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 98.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ectoine/hydroxyectoine ABC transporter solute-binding protein EhuB (TIGR02995; HMM-score: 59.7)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 46.1)
    extracellular substrate-binding orphan protein, GRRM family (TIGR04262; HMM-score: 41.1)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 29.9)
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 22.6)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 22.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.6)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 18.4)
    Hypothetical proteins Conserved conserved hypothetical protein (TIGR02285; HMM-score: 14.5)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 12)
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 12)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PBP (CL0177) SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 123.1)
    and 4 more
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 88)
    PBP (CL0177) Lig_chan-Glu_bd; Ligated ion channel L-glutamate- and glycine-binding site (PF10613; HMM-score: 35.3)
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 14.6)
    no clan defined DUF2085; Predicted membrane protein (DUF2085) (PF09858; HMM-score: 9.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9999
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0001
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.995819
    • TAT(Tat/SPI): 0.001914
    • LIPO(Sec/SPII): 0.001537
    • Cleavage Site: CS pos: 28-29. AHA-EQ. Pr: 0.9908
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELRVGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTGKIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKGTEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISNVKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISKYGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPMLVQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSLGLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISFDPFTPLLVAAALYFVLTFVLTRIMNMIEGRLNASD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: ArgR/AhrC (repression) regulon, CodY (repression) regulon
    ArgR/AhrC(TF)important in Arginine biosynthesis, Arginine degradation;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_001857&oldid=107498"