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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1916 [new locus tag: SACOL_RS09865 ]
  • pan locus tag?: SAUPAN004794000
  • symbol: SACOL1916
  • pan gene symbol?: glnP
  • synonym:
  • product: amino acid ABC transporter permease/substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1916 [new locus tag: SACOL_RS09865 ]
  • symbol: SACOL1916
  • product: amino acid ABC transporter permease/substrate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1969722..1971179
  • length: 1458
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAGTGTTTAATTAGGTTTATATTAGTATTAGGGTTGTTAATTAGCAGTGCTATGGTG
    TATATCAATCCCACAGCACATGCTGAACAAGATCAAACATGGGAGAAGATTAAAGAACGC
    GGTGAACTTAGAGTGGGTCTTTCTGCAGATTATGCACCAATGGAATTTGAGCATACAGTT
    AATGGTAAGACTGAGTATGCAGGTGTAGATATTGATTTAGCTAAAAAAATTGCGAAAGAT
    AATAATTTAAAATTAAAAATCGTCAATATGTCATTTGATAGTTTGTTAGGAGCTCTTAAA
    ACTGGAAAAATTGATATTATTATTTCCGGAATGACTTCAACGCCTGAACGTAAGAAGCAA
    GTTGATTTTTCAGATTCATATATGATGACTAAAAATATCATGCTTGTAAAGAAAGATAAA
    GTTAATGAATATAAAGATATCAAAGACTTTAATAATAAAAAAGTAGGGGCACAAAAGGGA
    ACTGAACAAGAAAAAATCGCTCAAACCGAAATTGAAAATGCATCTATTACTTCATTAAGC
    CGTTTACCAGACGTTATATTAGCATTGAAAAGTGGAAAGGTTGAAGGTGCTGTAGTTGAA
    AAACCTGTAGCAGAAGCATATTTAAAACAAAATCCTAAATTAGGAATTTCCAATGTGAAA
    TTTAATGAAGAAGAAAAAGATACAGTGATAGCAGTGCCAAAAGATTCACCAAAATTATTG
    TCACAAATTAATAAAACGATTAAGGAGGTTAAAGATAAAGGATTAATCGATAAATATATG
    ACTAATGCTGCAAATGCGATGAATGATGACAGTGGTTTTATTTCTAAGTATGGTAGTTTT
    TTCTTGAAAGGAATTAAGATTACAATATTAATTTCACTTATCGGTGTTGCATTAGGTTCT
    ATTTTAGGTGCATTCGTTGCGTTAATGAAATTAAGTAAAATAAAAATTATTTCATGGATT
    GCTTCTATCTATATCGAAATATTAAGAGGAACACCAATGTTAGTTCAAGTATTTATCGTA
    TTCTTTGGTATAACTGCTGCATTAGGACTAGACATTTCAGCTCTTGTTTGTGGAACAATT
    GCATTAGTTATTAATTCCTCAGCTTACATTGCTGAAATTATTCGTGCAGGTATAAATGCT
    GTTGATAAAGGCCAAATGGAAGCCGCACGTAGTCTAGGTTTGAATTATAGACAAACGATG
    AAAAGTGTAATTATGCCACAAGCAATTAAAAATATTTTACCAGCTTTAGGTAATGAATTT
    GTCACTTTAATTAAAGAATCATCTATTGTGTCAACAATTGGAGTTGGCGAAATTATGTTT
    AATGCACAAGTGGTTCAAGGTATTTCATTTGACCCATTCACACCATTAATAGTGGCAGCA
    GCATTATACTTTGTCTTAACATTTGTACTTACACGCATTATGAACATGATTGAAGGGAGA
    TTGAATGCCAGTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1458

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1916 [new locus tag: SACOL_RS09865 ]
  • symbol: SACOL1916
  • description: amino acid ABC transporter permease/substrate-binding protein
  • length: 485
  • theoretical pI: 9.56041
  • theoretical MW: 53122.2
  • GRAVY: 0.212371

⊟Function[edit | edit source]

  • TIGRFAM:
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 159)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 153.7)
    Metabolism Transport and binding proteins Amino acids, peptides and amines lysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 136.6)
    and 12 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 98.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ectoine/hydroxyectoine ABC transporter solute-binding protein EhuB (TIGR02995; HMM-score: 59.7)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 45.9)
    extracellular substrate-binding orphan protein, GRRM family (TIGR04262; HMM-score: 41.1)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 29.8)
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 22.6)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 22.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.7)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 18.2)
    Hypothetical proteins Conserved conserved hypothetical protein (TIGR02285; HMM-score: 14.5)
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 11.9)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 11.6)
  • TheSEED  :
    • Arginine ABC transporter, substrate-binding and permease protein
  • PFAM:
    PBP (CL0177) SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 123)
    and 4 more
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 88)
    PBP (CL0177) Lig_chan-Glu_bd; Ligated ion channel L-glutamate- and glycine-binding site (PF10613; HMM-score: 35.3)
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 14.6)
    no clan defined DUF2085; Predicted membrane protein (DUF2085) (PF09858; HMM-score: 9.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9999
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0001
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.995819
    • TAT(Tat/SPI): 0.001914
    • LIPO(Sec/SPII): 0.001537
    • Cleavage Site: CS pos: 28-29. AHA-EQ. Pr: 0.9908
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELRVGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTGKIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKGTEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISNVKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISKYGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPMLVQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSLGLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISFDPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: ArgR/AhrC (repression) regulon, CodY (repression) regulon
    ArgR/AhrC(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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