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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001656
  • pan locus tag?: SAUPAN004287000
  • symbol: hemA
  • pan gene symbol?: hemA
  • synonym:
  • product: glutamyl-tRNA reductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001656
  • symbol: hemA
  • product: glutamyl-tRNA reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 1689994..1691340
  • length: 1347
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCATTTTATTGCAATTAGTATAAATCATCGCACAGCTGATGTTGCACTAAGAGAGCAA
    GTTGCTTTTAGAGATGATGCCTTACGAATTGCCCATGAAGATTTATATGAAACTAAATCT
    ATTTTAGAAAATGTCATATTATCAACATGTAATCGAACTGAAGTATATGCTGTTGTTGAT
    CAAATTCACACAGGTCGTTACTATATTCAACGATTTCTAGCTCGTGCATTTGGATTTGAA
    GTAGATGATATTAAAGCAATGTCAGAAGTAAAAGTGGGGGACGAAGCAGTAGAACATTTA
    TTGCGTGTCACTTCTGGTTTAGATTCAATCGTACTTGGAGAAACTCAAATTTTAGGTCAA
    ATAAGAGATGCATTTTTCTTAGCGCAAAGCACAGGTACGACAGGAACAATTTTTAATCAT
    CTATTTAAACAGGCAATTACTTTTGCAAAAAGAGCACATAATGAAACAGATATAGCTGAT
    AATGCTGTAAGTGTGTCTTATGCTGCGGTCGAGTTGGCGAAAAAAGTATTTGGCAAATTG
    AAAAGTAAGCAAGCTATCATTATTGGTGCAGGGGAAATGAGTGAATTATCACTATTAAAT
    CTTCTTGGTTCTGGAATTACTGATATTACAGTAGTAAATAGAACAATTGAAAATGCTATG
    AAATTAGCAGCAAAGCATCAAGTGAAATATGATGAACTATCATCATTACCAAATTTACTT
    GAAAGTGCAGATATTGTGATTAGTTCAACGAGTGCACAATCTTATATCATTACAAATGAA
    ATGATAGAAAGAATTGCAGAAAATAGAAAGCAAGATTCACTAGTATTGATTGATATTGCA
    GTTCCTCGAGATATTGAACCTGGTATTAGTGCCATCACAAACATCTTTAATTATGATGTT
    GATGACTTAAAAGGTTTAGTTGATGCAAACTTACGTGAGCGACAATTAGCGGCTGCAACA
    ATTTCGGAACAAATTCCTGCAGAAATACATGCACACAATGAGTGGATTAGTATGTTGGGT
    GTTGTTCCAGTGATTAGAGCTTTACGTGAAAAAGCTATGGCAATTCAAGCAGAAACGATG
    GACAGTATTGATCGTAAATTGCCAGGGTTAAGCGAAAGAGAACGTAAAATTATATCTAAA
    CATACAAAAAGTATCATCAATCAAATGTTGAAAGATCCTATTAAACAGGCCAAAGAATTA
    AGTAGTGATAAGAAAAGTAATGAAAAATTAGAGCTATTTCAAAATATATTTGATATCGAA
    GCTGAATGTCCTCATGAGCAAGCGAAGCAACAAAAAGAAAGCAAGGTCAAGGAAATTTCA
    GCGCGACGTATCTTTAGTTTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1347

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001656
  • symbol: HemA
  • description: glutamyl-tRNA reductase
  • length: 448
  • theoretical pI: 5.81316
  • theoretical MW: 50099
  • GRAVY: -0.148884

⊟Function[edit | edit source]

  • reaction:
    EC 1.2.1.70?  ExPASy
    Glutamyl-tRNA reductase L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 449.5)
    and 5 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate dehydrogenase (TIGR00507; EC 1.1.1.25; HMM-score: 27.4)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 24.4)
    ectoine utilization protein EutC (TIGR02992; HMM-score: 22.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin siroheme synthase, N-terminal domain (TIGR01470; HMM-score: 15.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 12.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined GlutR_N; Glutamyl-tRNAGlu reductase, N-terminal domain (PF05201; HMM-score: 168.9)
    NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 154.3)
    and 8 more
    no clan defined GlutR_dimer; Glutamyl-tRNAGlu reductase, dimerisation domain (PF00745; HMM-score: 81.7)
    NADP_Rossmann (CL0063) NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 23.6)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 21.9)
    OCD_Mu_crystall; Ornithine cyclodeaminase/mu-crystallin family (PF02423; HMM-score: 19)
    THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 15.7)
    KARI_N; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 12.5)
    no clan defined DUF4837; Domain of unknown function (DUF4837) (PF16125; HMM-score: 10.4)
    HTH (CL0123) DUF6996; Domain of unknown function (DUF6996) (PF22515; HMM-score: 10.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9532
    • Cytoplasmic Membrane Score: 0.0153
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0311
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004119
    • TAT(Tat/SPI): 0.000347
    • LIPO(Sec/SPII): 0.001196
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILENVILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLRVTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIADNAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIENAMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVLIDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNEWISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKDPIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LacR* (repression) regulon
    LacR*(TF)important in Lactose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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