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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

organism: Staphylococcus aureus JSNZ
locus tag: JSNZ_001654
pan locus tag^?: SAUPAN004285000
symbol: hemC
pan gene symbol^?: hemC
synonym:
product: hydroxymethylbilane synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: JSNZ_001654
symbol: hemC
product: hydroxymethylbilane synthase
replicon: chromosome
strand: -
coordinates: 1688189..1689115
length: 927
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID:
RefSeq:
BioCyc:
MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCGTAAATTAGTCGTTGGCTCCAGAAGAAGTAAATTAGCTTTAACACAAAGCCAGCAA
TTTATTGATAAATTAAAAGCTGTCGAGCCAAATCTAGAAATTGAAATTAAAGAAATTGTC
ACGAAAGGCGATCGTATAGTAGATAAACAATTGTCTAAAGTCGGAGGCAAAGGCTTATTT
GTTAAAGAAATACAACATGAACTTTTTGAAAAAAATATCGATATGGCAATACACTCGCTT
AAAGACGTACCAAGTGTAATTCCGGAAGGTTTAACATTAGGTTGTATCCCTGATAGAGAA
TTACCTTTTGATGCGTATATTTCTAAAACACATACACCACTATCCCAATTGCCAGAAGGC
AGTATTATTGGTACTAGTTCATTACGTCGTGGTGCACAAATATTATCTAAGTATCCTAAT
TTAGAGATTAAATGGATTAGAGGTAATATAGATACACGATTAGAAAAGTTACAAACTGAA
GATTATGATGCGATTATTTTAGCTGCAGCTGGTTTAAGAAGAATGGGCTGGTCAGATGAT
ATTGTAACATCTTATCTTGATAGAGATACATTGTTACCTGCAATCGGACAAGGTGCTTTA
GGGATAGAATGTCGTAGTGACGATGAAGAACTATTAACATTATTAAGCAAAGTACATAAT
GATGAGGTTGCAAAATGTGTGACTGCTGAACGAACGTTTTTAGCAGAAATGGATGGTAGT
TGTCAGGTGCCAATTGCAGGATATGCTACAATCTCAGATCAAAACGAAATCGAATTTACA
GGTTTAATTATGACCCCAGATGGTAAAGAACGATTTGAATATACAATGAACGGAACAGAT
CCGGTTGAGTTAGGCAAAACAGTGAGTAACAAATTAAAAGAGCAAGGTGCTTATGAAATT
ATAAAACGCTTAAATGAACAACATTAA
60
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420
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540
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840
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927

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: JSNZ_001654
symbol: HemC
description: hydroxymethylbilane synthase
length: 308
theoretical pI: 5.04703
theoretical MW: 34354.1
GRAVY: -0.313312

⊟Function[edit | edit source]

reaction:
EC 2.5.1.61? ExPASy
Hydroxymethylbilane synthase 4 porphobilinogen + H₂O = hydroxymethylbilane + 4 NH₃
TIGRFAM:
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin hydroxymethylbilane synthase (TIGR00212; EC 2.5.1.61; HMM-score: 352.8)
TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
PFAM:
PBP (CL0177) Porphobil_deam; Porphobilinogen deaminase, dipyromethane cofactor binding domain (PF01379; HMM-score: 275.3)
and 1 more
no clan defined Porphobil_deamC; Porphobilinogen deaminase, C-terminal domain (PF03900; HMM-score: 85.5)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.999
- Cytoplasmic Membrane Score: 0.0001
- Cell wall & surface Score: 0
- Extracellular Score: 0.0009
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005228
- TAT(Tat/SPI): 0.000756
- LIPO(Sec/SPII): 0.000883
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI:
RefSeq:
UniProt:

⊟Protein sequence[edit | edit source]

MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGDRIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELPFDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTEDYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKVHNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTMNGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

Operon-mapper ^[1] : JSNZ_001649 < JSNZ_001650 < hemL < hemB < JSNZ_001653 < hemC < ccsA < hemA

⊟Regulation[edit | edit source]

regulator: LacR* (repression) regulon
LacR* (TF) important in Lactose utilization; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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