PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL1719 [new locus tag: SACOL_RS08780 ]
• pan locus tag^?: SAUPAN004287000
• symbol: hemA
• pan gene symbol^?: hemA
• synonym:
• product: glutamyl-tRNA reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL1719 [new locus tag: SACOL_RS08780 ]
• symbol: hemA
• product: glutamyl-tRNA reductase
• replicon: chromosome
• strand: -
• coordinates: 1749527..1750873
• length: 1347
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3236739 NCBI
• RefSeq: YP_186557 NCBI
• BioCyc: see SACOL_RS08780
• MicrobesOnline: 913166 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1321
  
  ATGCATTTTATTGCAATTAGTATAAATCATCGCACAGCTGATGTTGCACTAAGAGAGCAA
  GTTGCTTTTAGAGATGATGCCTTACGAATTGCCCATGAAGATTTATATGAAACTAAATCT
  ATTTTAGAAAATGTCATATTATCAACATGTAATCGAACTGAAGTATATGCTGTTGTTGAT
  CAAATTCACACAGGTCGTTACTATATTCAACGATTTCTAGCTCGTGCATTTGGATTTGAA
  GTAGATGATATTAAAGCAATGTCAGAAGTAAAAGTGGGGGACGAAGCAGTAGAACATTTA
  TTGCGTGTCACTTCTGGTTTAGATTCAATCGTACTTGGAGAAACTCAAATTTTAGGTCAA
  ATAAGAGATGCATTTTTCTTAGCGCAAAGCACAGGTACGACAGGAACAATTTTTAATCAT
  CTATTTAAACAGGCAATTACTTTTGCAAAAAGAGCACATAATGAAACAGATATAGCTGAT
  AATGCTGTAAGTGTGTCTTATGCTGCGGTCGAGTTGGCGAAAAAAGTATTTGGCAAATTG
  AAAAGTAAGCAAGCTATCATTATTGGTGCAGGGGAAATGAGTGAATTATCACTATTAAAT
  CTTCTTGGTTCTGGAATTACTGATATTACAGTAGTAAATAGAACAATTGAAAATGCTATG
  AAATTAGCAGCAAAGCATCAAGTGAAATATGATGAACTATCATCATTACCAAATTTACTT
  GAAAGTGCAGATATTGTGATTAGTTCAACGAGTGCACAATCTTATATCATTACAAATGAA
  ATGATAGAAAGAATTGCAGAAAATAGAAAGCAAGATTCACTAGTATTGATTGATATTGCA
  GTTCCTCGAGATATTGAACCTGGTATTAGTGCCATCACAAACATCTTTAATTATGATGTT
  GATGACTTAAAAGGTTTAGTTGATGCAAACTTACGTGAGCGACAATTAGCGGCTGCAACA
  ATTTCGGAACAAATTCCTGCAGAAATACATGCACACAATGAGTGGATTAGTATGTTGGGT
  GTTGTTCCAGTGATTAGAGCTTTACGTGAAAAAGCTATGGCAATTCAAGCAGAAACGATG
  GACAGTATTGATCGTAAATTGCCAGGGTTAAGCGAAAGAGAACGTAAAATTATATCTAAA
  CATACAAAAAGTATCATCAATCAAATGTTGAAAGATCCTATTAAACAGGCCAAAGAATTA
  AGTAGTGATAAGAAAAGTAATGAAAAATTAGAGCTATTTCAAAATATATTTGATATCGAA
  GCTGAATGTCCTCATGAGCAAGCGAAGCAACAAAAAGAAAGCAAGGTCAAGGAAATTTCA
  GCGCGACGTATCTTTAGTTTTGAATAA
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  1347
  

⊟Protein

⊟General

• locus tag: SACOL1719 [new locus tag: SACOL_RS08780 ]
• symbol: HemA
• description: glutamyl-tRNA reductase
• length: 448
• theoretical pI: 5.81316
• theoretical MW: 50099
• GRAVY: -0.148884

⊟Function

• reaction:
  EC 1.2.1.70?  ExPASy
  Glutamyl-tRNA reductase L-glutamate 1-semialdehyde + NADP⁺ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 449.5)
  and 5 more
  Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate dehydrogenase (TIGR00507; EC 1.1.1.25; HMM-score: 27.4)
  Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 24.4)
  ectoine utilization protein EutC (TIGR02992; HMM-score: 22.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin siroheme synthase, N-terminal domain (TIGR01470; HMM-score: 15.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 12.7)
• TheSEED  :
  • Glutamyl-tRNA reductase (EC 1.2.1.70)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles CPO analysis  Glutamyl-tRNA reductase (EC 1.2.1.70)
• PFAM:
  no clan defined GlutR_N; Glutamyl-tRNAGlu reductase, N-terminal domain (PF05201; HMM-score: 168.9)
  NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 154.3)
  and 8 more
  no clan defined GlutR_dimer; Glutamyl-tRNAGlu reductase, dimerisation domain (PF00745; HMM-score: 81.7)
  NADP_Rossmann (CL0063) NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 23.6)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 21.9)
  OCD_Mu_crystall; Ornithine cyclodeaminase/mu-crystallin family (PF02423; HMM-score: 19)
  THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 15.7)
  KARI_N; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 12.5)
  no clan defined DUF4837; Domain of unknown function (DUF4837) (PF16125; HMM-score: 10.4)
  HTH (CL0123) DUF6996; Domain of unknown function (DUF6996) (PF22515; HMM-score: 10.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9532
  • Cytoplasmic Membrane Score: 0.0153
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0311
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004119
  • TAT(Tat/SPI): 0.000347
  • LIPO(Sec/SPII): 0.001196
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57650544 NCBI
• RefSeq: YP_186557 NCBI
• UniProt: Q5HFA0 UniProt

⊟Protein sequence

• MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILENVILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLRVTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIADNAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIENAMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVLIDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNEWISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKDPIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hemL1 < hemB < hemD < hemC < SACOL1718 < hemA

⊟Regulation

• data available for JSNZ

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications