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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

organism: Staphylococcus aureus JSNZ
locus tag: JSNZ_001568
pan locus tag^?: SAUPAN004160000
symbol: JSNZ_001568
pan gene symbol^?: rsmE
synonym:
product: 16S rRNA (uracil(1498)-N(3))-methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: JSNZ_001568
symbol: JSNZ_001568
product: 16S rRNA (uracil(1498)-N(3))-methyltransferase
replicon: chromosome
strand: -
coordinates: 1603455..1604207
length: 753
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID:
RefSeq:
BioCyc:
MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
GTGCAACGTTATTTCATAGACCAAAACGCTGATGTAAGTCAGCGTTTTTTTATTACAAAA
AAAGAAGATATTCATCATATAACAAATGTAATGAGATATGATGTTGGTAGTAAAATTATT
TTAACTTTTAATGATCAAACAGTTTATTTATGTGAAATTGCTGATATAACAAGTGATAAA
ATTGCAGTTGATTTAAAAGAAAAACAAAATATTAATACAGAATTGCCAGTTGATGTTACG
ATTTGCAGTGGACTAATTAAAGCTGACAAATATGAGTGGTTGCTACAAAAAGCTACTGAA
TTGGGTGCTTCATCATTTATAGCTGTTAGCATGGAACGCTCAATTGTTAAATTAAACGAG
GCGAAAGTTGCCAAAAAGATAGAGCGTTGGCAAAAAATAATTAAAGAAGCTGCTGAACAA
AGCTATCGTTTAGTGATTCCATCTATTCAATTCGAGTCGAATTTAAAATTAATTTGTGAT
ACTATTGATAATTATGACTATATTCTTATTGCATATGAAGAGGAAGCAAAAGATGGTGAG
TTAAGCAATTTCAAGCAAACTTTACAACAATTCAAGGCTCAGGATAAAGTGTTGATGATT
TTTGGTCCTGAGGGTGGTTTGTCAGAAAATGAAATTTCATTATTTAGTAATACTAGTACA
GTTGTTGGTCTTGGACCGAGGATTTTACGGGCAGAAACTGCGCCATTGTATGCGTTAAGT
GCAATAAGTTATGAAAAAGAATTAATGGGGTGA
60
120
180
240
300
360
420
480
540
600
660
720
753

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: JSNZ_001568
symbol: JSNZ_001568
description: 16S rRNA (uracil(1498)-N(3))-methyltransferase
length: 250
theoretical pI: 4.74234
theoretical MW: 28425.4
GRAVY: -0.172

⊟Function[edit | edit source]

reaction:
EC 2.1.1.193? ExPASy
16S rRNA (uracil¹⁴⁹⁸-N³)-methyltransferase S-adenosyl-L-methionine + uracil¹⁴⁹⁸ in 16S rRNA = S-adenosyl-L-homocysteine + N³-methyluracil¹⁴⁹⁸ in 16S rRNA
TIGRFAM:
Genetic information processing Protein synthesis tRNA and rRNA base modification RNA methyltransferase, RsmE family (TIGR00046; EC 2.1.1.-; HMM-score: 204.3)
TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
PFAM:
SPOUT (CL0098) Methyltrans_RNA; RNA methyltransferase domain (PF04452; HMM-score: 185)
and 4 more
PUA (CL0178) PUA_4; RNA methyltransferase PUA domain (PF20260; HMM-score: 50.6)
SPOUT (CL0098) SpoU_methylase; SpoU rRNA Methylase family (PF00588; HMM-score: 14)
TPR (CL0020) NOT1_connector; CCR4-NOT transcription complex subunit 1, NOT1_connector (PF23590; HMM-score: 13.1)
bBprotInhib (CL0354) Staphostatin_A; Staphostatin A (PF09022; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9849
- Cytoplasmic Membrane Score: 0.0032
- Cell wall & surface Score: 0.0003
- Extracellular Score: 0.0115
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00151
- TAT(Tat/SPI): 0.000119
- LIPO(Sec/SPII): 0.000291
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI:
RefSeq:
UniProt:

⊟Protein sequence[edit | edit source]

MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFNDQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELGASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICDTIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNTSTVVGLGPRILRAETAPLYALSAISYEKELMG

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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