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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001567
  • pan locus tag?: SAUPAN004159000
  • symbol: mtaB
  • pan gene symbol?: mtaB
  • synonym:
  • product: tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001567
  • symbol: mtaB
  • product: tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB
  • replicon: chromosome
  • strand: -
  • coordinates: 1602102..1603448
  • length: 1347
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCAACAGTTGCGTTTCACACATTAGGTTGTAAAGTAAACCATTATGAAACTGAAGCA
    ATCTGGCAATTATTTAAAGAAGCAAACTATGAGCGCGTTGACTTTGAAGCGAATGCTGAT
    GTATTTGTTATTAATACTTGTACAGTAACGAATACGGGTGATAAAAAAAGTCGTCAAATA
    ATTAGACGTGCAATAAGACAAAATCCTGATGCTGTAATCTGTGTAACAGGTTGTTATGCG
    CAAACTTCATCAGCTGAAATTATGGAAATTCCTGGTGTCGATGTAGTAGTTGGTACACAA
    GATAGACATAAACTATTAGGTTACATTGATGAATTCCGTAAAGAACGCCAACCAATTAAT
    GGTGTTGGAAATATCATGAAAAATCGTAAATATGAAGAATTAGATGTCCCATATTTTACA
    GATAGAACACGTGCGTCATTAAAAATTCAAGAAGGTTGTAACAACTTCTGCACATTCTGT
    ATTATTCCATGGGCTCGTGGCTTAATGCGTTCAAGAGATCCGGAAAAAGTAGTTGAACAA
    GCGACGCAACTAGTGAATTCAGGATATAAGGAAATTGTATTGACGGGAATTCATACAGGT
    GGATATGGTCAAGATTTAAAAGATTATAACTTGGCCCAATTATTACGTGATCTTGAAACG
    ATTAATGGATTAGAACGAATTCGAATTTCTTCAATTGAAGCAAGTCAACTTACAGATGAA
    GTAATTGACGTTTTAGAACGTTCAACAAAAGTTGTGCGTCATTTGCATATTCCATTACAA
    TCTGGTTCAGATACAGTATTAAAACGTATGAGACGTAAGTATACAATGGATAGATTTTCA
    GAACGATTAACAAAATTGCATAAAGCTTTACCAGACTTGGCAGTTACGAGTGATGTAATT
    GTTGGTTTCCCAGGTGAAACTGAAGCAGAGTTCCAAGAAACATATGATTTTATCGTAAAG
    CATAAGTTCTCTGAACTGCATGTTTTCCCTTATTCTCCTAGAATTGGCACGCCAGCTGCA
    AGAATGGATGACCAAATTGATGAAGAAATTAAAAATGAACGCGTGCATAAGTTAATTACG
    CTAAGCAATCAACTCGGAAAGTTATATGCGTCTAAATTTGATCAAGATGTGCTTGAAGTA
    ATTCCTGAGGAACAGGGTGACACAGAAGGTACATTAGTTGGATATGCAGATAATTATATG
    AAAGTACAATTTGAAGGTGACGAATCACTCATAGGTCAAATTGTAAAAGTTAAAATTACG
    CAAGCAAATTATCCATTAAATGAAGGGCAAGCAATTAAAGTTGTTGATTTCGCAACAAAT
    AAATCTGATAGAGAAGTTTTAGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1347

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001567
  • symbol: MtaB
  • description: tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB
  • length: 448
  • theoretical pI: 5.53724
  • theoretical MW: 50955.5
  • GRAVY: -0.397321

⊟Function[edit | edit source]

  • TIGRFAM:
    radical SAM methylthiotransferase, MiaB/RimO family (TIGR00089; EC 2.1.1.-,2.8.1.-; HMM-score: 494.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 476.1)
    and 23 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-i(6)A37 thiotransferase enzyme MiaB (TIGR01574; EC 2.-.-.-; HMM-score: 354.1)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein S12 methylthiotransferase RimO (TIGR01125; EC 2.1.1.-,2.8.1.-; HMM-score: 295.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme, archaeal-type (TIGR01578; HMM-score: 276.4)
    Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_1487 family (TIGR04013; HMM-score: 95.7)
    Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_0865 family (TIGR04014; HMM-score: 82.8)
    Cellular processes Cellular processes Toxin production and resistance radical SAM P-methyltransferase, PhpK family (TIGR04479; EC 2.1.-.-; HMM-score: 52.1)
    hopanoid biosynthesis associated radical SAM protein HpnJ (TIGR03471; HMM-score: 39.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin coproporphyrinogen dehydrogenase HemZ (TIGR03994; EC 1.3.99.22; HMM-score: 33.3)
    Cellular processes Cellular processes Adaptations to atypical conditions KamA family protein (TIGR00238; HMM-score: 29.6)
    Unknown function Enzymes of unknown specificity uncharacterized radical SAM protein YgiQ (TIGR03904; HMM-score: 29.3)
    Unknown function Enzymes of unknown specificity radical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 27.6)
    Unknown function Enzymes of unknown specificity radical SAM family uncharacterized protein (TIGR03960; HMM-score: 25.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Lipoate lipoyl synthase (TIGR00510; EC 2.8.1.8; HMM-score: 24.1)
    magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (TIGR02026; HMM-score: 20.8)
    Cellular processes Cellular processes Toxin production and resistance tryptophan 2-C-methyltransferase (TIGR04428; EC 2.1.1.106; HMM-score: 19.9)
    Cellular processes Cellular processes Adaptations to atypical conditions glutamate 2,3-aminomutase (TIGR04368; EC 5.4.3.9; HMM-score: 19.8)
    putative heme utilization radical SAM enzyme HutW (TIGR04107; HMM-score: 19)
    lysine-2,3-aminomutase-related protein (TIGR03822; EC 5.4.3.-; HMM-score: 15.7)
    His-Xaa-Ser system radical SAM maturase HxsC (TIGR03977; HMM-score: 15.3)
    Genetic information processing Protein fate Protein modification and repair EF-P beta-lysylation protein EpmB (TIGR03821; EC 5.4.3.-; HMM-score: 15.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin oxygen-independent coproporphyrinogen III oxidase (TIGR00538; EC 1.3.99.22; HMM-score: 13.4)
    lysine-2,3-aminomutase (TIGR03820; EC 5.4.3.2; HMM-score: 13.4)
    B12-binding domain/radical SAM domain protein, rhizo-twelve system (TIGR04295; HMM-score: 11.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 107.7)
    CheY (CL0304) UPF0004; Uncharacterized protein family UPF0004 (PF00919; HMM-score: 98.1)
    and 2 more
    OB (CL0021) TRAM; TRAM domain (PF01938; HMM-score: 30.2)
    no clan defined B12-binding; B12 binding domain (PF02310; HMM-score: 19)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9895
    • Cytoplasmic Membrane Score: 0.0084
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0019
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010218
    • TAT(Tat/SPI): 0.000499
    • LIPO(Sec/SPII): 0.003792
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVINTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDRHKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFCIIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDLETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTMDRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPRIGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTLVGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: HrcA (repression) regulon
    HrcA(TF)important in Heat shock response;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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