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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001570
  • pan locus tag?: SAUPAN004162000
  • symbol: dnaJ
  • pan gene symbol?: dnaJ
  • synonym:
  • product: molecular chaperone DnaJ

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001570
  • symbol: dnaJ
  • product: molecular chaperone DnaJ
  • replicon: chromosome
  • strand: -
  • coordinates: 1605151..1606290
  • length: 1140
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    GTGGCCAAAAGAGACTATTATGAAGTTTTAGGCATTAGTAAAGATGCTTCAAAAGATGAA
    ATCAAAAAAGCGTATCGAAAGCTTTCGAAAAAATATCATCCAGATATTAACAAAGAAGAA
    GGTGCAGATGAAAAGTTTAAAGAAATTTCTGAAGCCTATGAAGTTTTAAGTGATGATAAT
    AAACGCGCAAGCTATGATCAATTTGGACATGATGGTCCACAAGGTTTTGGTGGACAAGGA
    TTCAATGGCTCTGACTTTGGCGGTTTTAGTGGCTTCGGTGGCGGCGGTTTTGAAGATATC
    TTTAGCTCTTTCTTCGGTGGCGGCAGACAAAGAGATCCAAATGCACCGCAAAAAGGTGAT
    GATCTTCAGTATACAATGACATTAACATTTGAAGAAGCGGTATTTGGTACAACAAAAGAA
    ATTTCAATCCGTAAAGATGTAACATGCGAAACATGTCATGGTGATGGTGCAAAGCCTGGA
    ACAAGTAAAAAGACTTGTAGTTACTGTAATGGCGCTGGTCATGTAGCTGTAGAACAAAAC
    ACAATTTTAGGTAGAGTTCGTACTGAACAAGTTTGTCCTAAATGTAATGGAAGTGGTCAA
    GAATTTGAAGAAGCTTGTCCAACATGTCACGGAAAAGGTACTGAAAATAAAACAGTTAAA
    TTAGAAGTGAAAGTACCTGAAGGTGTCGACAATGAACAACAAATTAGATTAGCTGGTGAA
    GGCTCTCCAGGCGTGAATGGAGGACCTGCTGGTGATTTATACGTAGTATTTAGAGTTAAA
    CCATCTGAAACATTTAAACGTGATGGTGATGATATTTATTACAAATTAAATGTTAGCTTC
    CCACAAGCAGCTTTAGGCGATGAAATAAAAATACCAACATTAAATAATGAAGTTATGTTA
    ACAATCCCTGCTGGTACACAAACAGGTAAGCAATTCCGCTTAAAAGAAAAGGGTATAAAA
    AATGTTCATGGCTATGGATACGGAGATTTATATGTTGATATTAAAGTAGTAACGCCTACT
    AAATTAACAGATAGACAAAAAGAACTAATGAAAGAATTTGCCCAATTAAATGGTGAAGAA
    ATTAATGAACAACCTTCGAATTTTAAAGATAGAGCAAAAAGATTCTTTAAGGGAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001570
  • symbol: DnaJ
  • description: molecular chaperone DnaJ
  • length: 379
  • theoretical pI: 5.79
  • theoretical MW: 41760.2
  • GRAVY: -0.783641

⊟Function[edit | edit source]

  • TIGRFAM:
    chaperone protein DnaJ (TIGR02349; HMM-score: 466)
    and 4 more
    Cellular processes Cellular processes Chemotaxis and motility terminal organelle assembly protein TopJ (TIGR03835; HMM-score: 157)
    Genetic information processing Protein fate Protein folding and stabilization Fe-S protein assembly co-chaperone HscB (TIGR00714; HMM-score: 21.4)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions uncharacterized phage protein (TIGR02642; HMM-score: 14.5)
    Genetic information processing Protein fate Protein modification and repair hydrogenase nickel insertion protein HypA (TIGR00100; HMM-score: 5.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined DnaJ_C; DnaJ C terminal domain (PF01556; HMM-score: 182.1)
    and 19 more
    Chaperone-J (CL0392) DnaJ; DnaJ domain (PF00226; HMM-score: 99.5)
    no clan defined DnaJ_CXXCXGXG; DnaJ central domain (PF00684; HMM-score: 60.3)
    C2H2-zf (CL0361) zf-C2H2_10; C2H2 zinc-finger (PF16588; HMM-score: 20.2)
    no clan defined Anti-TRAP; Tryptophan RNA-binding attenuator protein inhibitory protein (PF15777; HMM-score: 20)
    TackOD1; Thaumarchaeal output domain 1 (PF18551; HMM-score: 18)
    DUF5351; Family of unknown function (DUF5351) (PF17302; HMM-score: 15.9)
    PAS_Fold (CL0183) Tll0287-like; Tll0287 (PF11845; HMM-score: 14.8)
    TRASH (CL0175) zf-FCS; MYM-type Zinc finger with FCS sequence motif (PF06467; HMM-score: 12.8)
    Multiheme_cytos (CL0317) Cytochrom_c3_2; Cytochrome c3 (PF14537; HMM-score: 12.4)
    Cytochrome_C554; Cytochrome c554 and c-prime (PF13435; HMM-score: 12.3)
    Mtrc-MtrF_II-IV_dom; Outer membrane cytochrome MtrC/MtrF-like, domains II/IV (PF22113; HMM-score: 11.5)
    Cytochrome-c (CL0318) Cytochrome_CBB3; Cytochrome C oxidase, cbb3-type, subunit III (PF13442; HMM-score: 10.7)
    no clan defined TMEM106_N; Transmembrane protein 106 N-terminal region (PF21002; HMM-score: 10.1)
    HTH (CL0123) GeBP-like_DBD; Glabrous-enhancer-binding protein-like family, DBD domain (PF04504; HMM-score: 9.9)
    no clan defined RNase_E_G_Thio; RNase E/G, Thioredoxin-like domain (PF20833; HMM-score: 9.8)
    Multiheme_cytos (CL0317) Cytochrome_C7; Cytochrome c7 and related cytochrome c (PF14522; HMM-score: 9.7)
    Cytochrome-c (CL0318) DUF1924; Domain of unknown function (DUF1924) (PF09086; HMM-score: 7.8)
    Zn_Beta_Ribbon (CL0167) HypA; Hydrogenase/urease nickel incorporation, metallochaperone, hypA (PF01155; HMM-score: 7.4)
    RING (CL0229) zf-RING_10; zinc RING finger of MSL2 (PF16685; HMM-score: 6.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9958
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0034
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007689
    • TAT(Tat/SPI): 0.001249
    • LIPO(Sec/SPII): 0.00262
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADEKFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFSSFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPGTSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKTVKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKLNVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDIKVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CtsR* (repression) regulon, HrcA (repression) regulon
    CtsR*(TF)important in Heat shock response;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    HrcA(TF)important in Heat shock response;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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