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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001535
  • pan locus tag?: SAUPAN004123000
  • symbol: comGA
  • pan gene symbol?: comGA
  • synonym:
  • product: competence type IV pilus ATPase ComGA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001535
  • symbol: comGA
  • product: competence type IV pilus ATPase ComGA
  • replicon: chromosome
  • strand: -
  • coordinates: 1573130..1574104
  • length: 975
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTA
    CATTTTATTCCAGTTAAAAATGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAG
    CAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTATTAGTTTATATGAAGTTTCAA
    GCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
    AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGT
    GTTATCAGAATTGTACCTCAATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGAT
    TTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGTTTAGTGGGCCAACTGGTTCA
    GGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
    GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTG
    AATGATAAAGCTGGCATTAACTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCT
    GATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTGCCAAGTGTGTTATACATGCT
    AGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
    TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATT
    ATAAACCAACGACTTGTAACTACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTA
    TCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATTCATTACCATCATCATTTAAG
    AACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
    GATAAATACATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001535
  • symbol: ComGA
  • description: competence type IV pilus ATPase ComGA
  • length: 324
  • theoretical pI: 8.90863
  • theoretical MW: 36906.7
  • GRAVY: -0.0592593

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Pathogenesis type II secretion system protein E (TIGR02533; HMM-score: 219.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein E (TIGR02533; HMM-score: 219.9)
    Cell structure Cell envelope Surface structures type IV-A pilus assembly ATPase PilB (TIGR02538; HMM-score: 215.6)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type IV-A pilus assembly ATPase PilB (TIGR02538; HMM-score: 215.6)
    and 14 more
    Cell structure Cell envelope Surface structures twitching motility protein (TIGR01420; HMM-score: 150.1)
    Cellular processes Cellular processes Chemotaxis and motility twitching motility protein (TIGR01420; HMM-score: 150.1)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid transfer ATPase TraJ (TIGR02525; HMM-score: 84.9)
    Cellular processes Cellular processes Conjugation P-type conjugative transfer ATPase TrbB (TIGR02782; HMM-score: 57.3)
    Dot/Icm secretion system ATPase DotB (TIGR02524; HMM-score: 57.1)
    P-type DNA transfer ATPase VirB11 (TIGR02788; HMM-score: 54.7)
    helicase/secretion neighborhood ATPase (TIGR03819; HMM-score: 36.3)
    Cellular processes Cellular processes Sporulation and germination stage III sporulation protein AA (TIGR02858; HMM-score: 17.3)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 15.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 14.6)
    Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 13.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 12.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 12.2)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions conjugative transfer ATPase, PFL_4706 family (TIGR03744; HMM-score: 10.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    P-loop_NTPase (CL0023) T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 251.5)
    and 21 more
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 25.8)
    nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 19.7)
    Rad17; Rad17 P-loop domain (PF03215; HMM-score: 18.7)
    DAP3; Mitochondrial ribosomal death-associated protein 3 (PF10236; HMM-score: 18.3)
    AAA_14; AAA domain (PF13173; HMM-score: 17.6)
    AAA_7; P-loop containing dynein motor region (PF12775; HMM-score: 17.2)
    NPHP3_N; Nephrocystin 3, N-terminal (PF24883; HMM-score: 17.2)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.9)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 16.2)
    AAA_23; AAA domain (PF13476; HMM-score: 16.2)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 15.2)
    AAA_22; AAA domain (PF13401; HMM-score: 15.2)
    ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 15)
    AAA_30; AAA domain (PF13604; HMM-score: 15)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 14.5)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 13.3)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 13.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.1)
    DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 12.5)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 12.4)
    AAA_25; AAA domain (PF13481; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.6054
    • Cytoplasmic Membrane Score: 0.3813
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0133
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003912
    • TAT(Tat/SPI): 0.000073
    • LIPO(Sec/SPII): 0.000327
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLNVISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIHASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigH* regulon
    SigH*(sigma factor)controlling competence and phage integrase genes; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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