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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01643
  • pan locus tag?: SAUPAN004123000
  • symbol: SAOUHSC_01643
  • pan gene symbol?: comGA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01643
  • symbol: SAOUHSC_01643
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1559316..1560290
  • length: 975
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTA
    CATTTTATTCCAGTTAAAAATGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAG
    CAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTATTAGTTTATATGAAGTTTCAA
    GCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
    AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGT
    GTTATCAGAATTGTACCTCAATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGAT
    TTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGTTTAGTGGACCAACTGGTTCA
    GGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
    GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTG
    AATGATAAAGCTGGCATAAACTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCT
    GATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTGCCAAGTGTGTTATACAGGCT
    AGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
    TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATT
    ATAAACCAACGACTTGTAACTACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTA
    TCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATTCATTACCATCATCATTTAAG
    AACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
    CATAAATACATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01643
  • symbol: SAOUHSC_01643
  • description: hypothetical protein
  • length: 324
  • theoretical pI: 9.07987
  • theoretical MW: 36864.8
  • GRAVY: -0.0317901

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cellular processes Cellular processes Pathogenesis type II secretion system protein E (TIGR02533; HMM-score: 221.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein E (TIGR02533; HMM-score: 221.8)
    Cell structure Cell envelope Surface structures type IV-A pilus assembly ATPase PilB (TIGR02538; HMM-score: 213.5)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type IV-A pilus assembly ATPase PilB (TIGR02538; HMM-score: 213.5)
    and 14 more
    Cell structure Cell envelope Surface structures twitching motility protein (TIGR01420; HMM-score: 146.5)
    Cellular processes Cellular processes Chemotaxis and motility twitching motility protein (TIGR01420; HMM-score: 146.5)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid transfer ATPase TraJ (TIGR02525; HMM-score: 84.9)
    Dot/Icm secretion system ATPase DotB (TIGR02524; HMM-score: 58.2)
    Cellular processes Cellular processes Conjugation P-type conjugative transfer ATPase TrbB (TIGR02782; HMM-score: 57.5)
    P-type DNA transfer ATPase VirB11 (TIGR02788; HMM-score: 54.5)
    helicase/secretion neighborhood ATPase (TIGR03819; HMM-score: 35.9)
    Cellular processes Cellular processes Sporulation and germination stage III sporulation protein AA (TIGR02858; HMM-score: 17.1)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 14.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 14.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 14.1)
    Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 12.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 12.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 12.2)
  • ⊞TheSEED  :
    • Late competence protein ComGA, access of DNA to ComEA
    DNA Metabolism DNA uptake, competence Gram Positive Competence  Late competence protein ComGA, access of DNA to ComEA
    and 1 more
    DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComGA, access of DNA to ComEA
  • ⊞PFAM:
    P-loop_NTPase (CL0023) T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 251.4)
    and 21 more
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 25.7)
    nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 19.7)
    Rad17; Rad17 P-loop domain (PF03215; HMM-score: 18.7)
    DAP3; Mitochondrial ribosomal death-associated protein 3 (PF10236; HMM-score: 18.2)
    AAA_7; P-loop containing dynein motor region (PF12775; HMM-score: 18)
    AAA_14; AAA domain (PF13173; HMM-score: 17.6)
    NPHP3_N; Nephrocystin 3, N-terminal (PF24883; HMM-score: 17.2)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.3)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 16.2)
    AAA_23; AAA domain (PF13476; HMM-score: 15.9)
    AAA_22; AAA domain (PF13401; HMM-score: 15.2)
    ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 15.1)
    AAA_30; AAA domain (PF13604; HMM-score: 14.9)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 14.4)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 14.4)
    G-alpha; G-protein alpha subunit (PF00503; HMM-score: 14)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 13.2)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 13.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 13)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 12.5)
    AAA_25; AAA domain (PF13481; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.6324
    • Cytoplasmic Membrane Score: 0.3537
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0139
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003912
    • TAT(Tat/SPI): 0.000073
    • LIPO(Sec/SPII): 0.000327
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLNVISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser:  [1] 
    Expression Data Browser
    ⊟Multi-gene expression profiles



    Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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