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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001496
  • pan locus tag?: SAUPAN004044000
  • symbol: JSNZ_001496
  • pan gene symbol?: malR
  • synonym:
  • product: LacI family DNA-binding transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001496
  • symbol: JSNZ_001496
  • product: LacI family DNA-binding transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 1539907..1540926
  • length: 1020
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTTACGATTAAAGATGTTGCACTAAAAGCCGGTGTTTCTCCTTCGACAGTTTCAAGA
    GTTATAAAAGGAAATCAACGTATTAGCGAAGCGACAATTTCAAAAGTGAAGAAAGTTATG
    GAAGAATTGAATTATTTTCCTAATACTGCTGCTAGAACCTTAATTACAAACCAAACATAT
    AAAATTGGTTTAGTGTTAAAAGGGTCTGAGGAGCCTATTCGACTGAATCCATTCTACATC
    AATGTATTGCTAGGAATTTCTGAAACGTGTAACCAGCATGGTTATGGTACACAAACGACA
    GTCTCAAATAATATGAATGATTTAATGGATGAAGTTTATAAAATGATTAAACAACGAATG
    GTTGATGCGTTTATTCTGCTCTATTCAAAAGAAAATGATCCGATTAAACAAATGTTAATT
    GATGAAAGCATGCCATTTATTGTGATTGGTAAGCCTACATCGGATATAGATCATCAATTT
    ACACACATAGATAATGATAATATATTAGCTTCTGAAAATTTGACACGACATGTTATTGAA
    CAAGGTGTAGATGAATTAATATTTATTACAGAAAAAGGCGATTTTGAAGTTTCGAAAGAC
    AGGATTCAAGGATTTGAAACGATAGCTTCTCAAAATAAAATTAATTATCAAATTATTGAG
    ACTAGTAATGAAAGAGAAGTTATTTTAAATTACATGCAAAATCTACATACGCGTTTGAAG
    GACCCAAATATTAAACAGGCAATCATTTCGTTAGATGCTATGTTACATTTAGCGATTTTA
    AGTGTCCTATATGAACTTAATATTGAAATTCCGAAAGATGTAATGACAGCAACGTTCAAT
    GATTCTTATTTAACCGAAATTGCGTCACCACCTCAAACCTGTATTGACATCAAGCCTCGA
    ATGTTAGGTCAACAGGCTGGTTCAGCAATTTTAAATATATTGAAAAACAAAGCACAGGAT
    GTTATTGAACTCGTCATTATAGATACAGAATTAAAAATAAGAAAATCAACACAGCGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001496
  • symbol: JSNZ_001496
  • description: LacI family DNA-binding transcriptional regulator
  • length: 339
  • theoretical pI: 5.46166
  • theoretical MW: 38373
  • GRAVY: -0.183776

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 115.3)
    Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 112.3)
    and 5 more
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 53)
    Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16)
    Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16)
    Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16)
    Hypothetical proteins Conserved TIGR02147 family protein (TIGR02147; HMM-score: 14.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 76.3)
    Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 70)
    and 10 more
    Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 59.6)
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 26.8)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 17.1)
    HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 17)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 16.1)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 15.3)
    HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 14.1)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 13.1)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 12.2)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9606
    • Cytoplasmic Membrane Score: 0.0233
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0157
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011903
    • TAT(Tat/SPI): 0.002449
    • LIPO(Sec/SPII): 0.00132
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELNYFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVSNNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQFTHIDNDNILASENLTRHVIEQGVDELIFITEKGDFEVSKDRIQGFETIASQNKINYQIIETSNEREVILNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMTATFNDSYLTEIASPPQTCIDIKPRMLGQQAGSAILNILKNKAQDVIELVIIDTELKIRKSTQR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: MalR* (repression) regulon, CcpA regulon
    MalR*(TF)important in Maltose utilization, Maltodextrin utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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