PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001496
• pan locus tag^?: SAUPAN004044000
• symbol: JSNZ_001496
• pan gene symbol^?: malR
• synonym:
• product: LacI family DNA-binding transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001496
• symbol: JSNZ_001496
• product: LacI family DNA-binding transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 1539907..1540926
• length: 1020
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGGTTACGATTAAAGATGTTGCACTAAAAGCCGGTGTTTCTCCTTCGACAGTTTCAAGA
  GTTATAAAAGGAAATCAACGTATTAGCGAAGCGACAATTTCAAAAGTGAAGAAAGTTATG
  GAAGAATTGAATTATTTTCCTAATACTGCTGCTAGAACCTTAATTACAAACCAAACATAT
  AAAATTGGTTTAGTGTTAAAAGGGTCTGAGGAGCCTATTCGACTGAATCCATTCTACATC
  AATGTATTGCTAGGAATTTCTGAAACGTGTAACCAGCATGGTTATGGTACACAAACGACA
  GTCTCAAATAATATGAATGATTTAATGGATGAAGTTTATAAAATGATTAAACAACGAATG
  GTTGATGCGTTTATTCTGCTCTATTCAAAAGAAAATGATCCGATTAAACAAATGTTAATT
  GATGAAAGCATGCCATTTATTGTGATTGGTAAGCCTACATCGGATATAGATCATCAATTT
  ACACACATAGATAATGATAATATATTAGCTTCTGAAAATTTGACACGACATGTTATTGAA
  CAAGGTGTAGATGAATTAATATTTATTACAGAAAAAGGCGATTTTGAAGTTTCGAAAGAC
  AGGATTCAAGGATTTGAAACGATAGCTTCTCAAAATAAAATTAATTATCAAATTATTGAG
  ACTAGTAATGAAAGAGAAGTTATTTTAAATTACATGCAAAATCTACATACGCGTTTGAAG
  GACCCAAATATTAAACAGGCAATCATTTCGTTAGATGCTATGTTACATTTAGCGATTTTA
  AGTGTCCTATATGAACTTAATATTGAAATTCCGAAAGATGTAATGACAGCAACGTTCAAT
  GATTCTTATTTAACCGAAATTGCGTCACCACCTCAAACCTGTATTGACATCAAGCCTCGA
  ATGTTAGGTCAACAGGCTGGTTCAGCAATTTTAAATATATTGAAAAACAAAGCACAGGAT
  GTTATTGAACTCGTCATTATAGATACAGAATTAAAAATAAGAAAATCAACACAGCGATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
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  720
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  840
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  960
  1020
  

⊟Protein

⊟General

• locus tag: JSNZ_001496
• symbol: JSNZ_001496
• description: LacI family DNA-binding transcriptional regulator
• length: 339
• theoretical pI: 5.46166
• theoretical MW: 38373
• GRAVY: -0.183776

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 115.3)
  Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 112.3)
  and 5 more
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 53)
  Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16)
  Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16)
  Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16)
  Hypothetical proteins Conserved TIGR02147 family protein (TIGR02147; HMM-score: 14.1)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 76.3)
  Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 70)
  and 10 more
  Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 59.6)
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 26.8)
  HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 17.1)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 17)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 16.1)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 15.3)
  HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 14.1)
  HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 13.1)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 12.2)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 11.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by MalR, TF important in Maltose utilization, Maltodextrin utilization: in N315

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9606
  • Cytoplasmic Membrane Score: 0.0233
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0157
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011903
  • TAT(Tat/SPI): 0.002449
  • LIPO(Sec/SPII): 0.00132
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELNYFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVSNNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQFTHIDNDNILASENLTRHVIEQGVDELIFITEKGDFEVSKDRIQGFETIASQNKINYQIIETSNEREVILNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMTATFNDSYLTEIASPPQTCIDIKPRMLGQQAGSAILNILKNKAQDVIELVIIDTELKIRKSTQR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications