PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1415 [new locus tag: NWMN_RS07985 ]
• pan locus tag^?: SAUPAN004044000
• symbol: marR
• pan gene symbol^?: malR
• synonym:
• product: maltose operon transcriptional repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1415 [new locus tag: NWMN_RS07985 ]
• symbol: marR
• product: maltose operon transcriptional repressor
• replicon: chromosome
• strand: -
• coordinates: 1584675..1585694
• length: 1020
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332116 NCBI
• RefSeq: YP_001332449 NCBI
• BioCyc:
• MicrobesOnline: 3706967 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGTTACGATTAAAGATGTTGCACTAAAAGCCGGTGTTTCTCCTTCGACAGTTTCAAGA
  GTTATAAAAGGAAATCAACGTATTAGCGAAGCGACAATTTCAAAAGTGAAGAAAGTTATG
  GAAGAATTGAATTATTTTCCTAATACCGCTGCTAGAACTTTAATTACAAACCAAACATAT
  AAAATTGGTTTAGTGTTAAAAGGGTCTGAGGAGCCTATTCGACTGAATCCATTCTACATC
  AATGTATTGCTAGGAATTTCTGAAACGTGTAACCAGCATGGCTATGGTACACAAACGACA
  GTCTCAAATAATATGAATGATTTAATGGATGAAGTTTATAAAATGATTAAACAACGAATG
  GTTGATGCGTTTATACTGCTCTATTCAAAAGAAAATGATCCGATTAAACAAATGTTAATT
  GATGAAAGCATGCCATTTATTGTGATTGGTAAGCCTACATCGGATATAGATCATCAATTT
  ACACATATAGATAATGATAATATATTAGCTTCTGAAAATTTGACACGACATGTTATTGAA
  CAAGGTGTAGATGAATTAATATTTATTACAGAAAAAGGAAATTTTGAAGTTTCAAAAGAT
  AGAATTCAAGGATTTGAAACGATAGCTTCTCAAAATAAAATTAATTATCAAATTATTGAG
  ACTAGTAATGAAAGAGAAGTTATTTTTAATTACATGCAAAATCTACATACGCGTTTGAAG
  GACCCAAATATTAAACAGGCAATCATTTCGTTAGATGCTATGTTACATTTAGCGATTTTA
  AGTGTCCTATATGAACTTAATATTGAAATTCCGAAAGATGTAATGACAGCAACGTTCAAT
  GATTCTTATTTAACCGAAATTGCGTCACCACCACAAACCTGTATTGACATCAAGCCTCGA
  ATGTTAGGTCAACAGGCTGGTGCAGCAATTTTAAATATATTGAAAAACAAAGCACAGGAT
  GTTATTGAACTCGTCATTATAGATACAGAATTAAAAATAAGAAAATCAACACAGCGATAG
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⊟Protein

⊟General

• locus tag: NWMN_1415 [new locus tag: NWMN_RS07985 ]
• symbol: MarR
• description: maltose operon transcriptional repressor
• length: 339
• theoretical pI: 5.7117
• theoretical MW: 38390.1
• GRAVY: -0.179056

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 117.9)
  Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 111.6)
  and 5 more
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 50.9)
  Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.2)
  Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.2)
  Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.2)
  Hypothetical proteins Conserved TIGR02147 family protein (TIGR02147; HMM-score: 14.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 75.8)
  Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 68.4)
  Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 61.3)
  and 13 more
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 27.5)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 17.5)
  HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 16.4)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 15.3)
  YdaS_antitoxin; Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT (PF15943; HMM-score: 14.9)
  HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 13.7)
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 13.4)
  HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 12.5)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 12.5)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 12.5)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 12.5)
  no clan defined DUF5351; Family of unknown function (DUF5351) (PF17302; HMM-score: 11.9)
  HTH (CL0123) MerR; MerR family regulatory protein (PF00376; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effector: Maltose
• genes regulated by MalR*, TF important in Maltose utilization, Maltodextrin utilizationRegPrecise

  repression

  malK* > malE* > malF* > NWMN_0154 > NWMN_0155 > NWMN_0156

  malA < marR

  transcription units transferred from N315 data RegPrecise

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011903
  • TAT(Tat/SPI): 0.002449
  • LIPO(Sec/SPII): 0.00132
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221627 NCBI
• RefSeq: YP_001332449 NCBI
• UniProt: A0A0H3KCV2 UniProt

⊟Protein sequence

• MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELNYFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVSNNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQFTHIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQIIETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMTATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIRKSTQR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: malA < marR

⊟Regulation

• regulators: CcpA regulon, MalR* (repression) regulon

  CcpA	(TF)	important in Carbon catabolism; RegPrecise
  MalR*	(TF)	important in Maltose utilization, Maltodextrin utilization; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications