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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0155 [new locus tag: NWMN_RS00845 ]
  • pan locus tag?: SAUPAN001069000
  • symbol: NWMN_0155
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0155 [new locus tag: NWMN_RS00845 ]
  • symbol: NWMN_0155
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 196032..197111
  • length: 1080
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATGACGATTAAAGTTGGAATCATTGGGTGTGGTGGTATTGCGAATGGCAAGCACATG
    CCAAGTTTACAAAAAGTTGAAAATGTTGAAATGATCGCATTTTGTGACGTAGACATTTCG
    AAAGCAGCGAGTGCGGCAGAAGCATACGGAACTGACAATGCAAAGGTTTATGATGATTAC
    AAAGCATTGTTAAAAGATGACACGATTGATGTTATCCATGTTTGTACGCCAAATGACTCG
    CATTGTGAAATTACTGTAGCAGGGCTGCATGCTGGTAAACATGTGATGTGTGAAAAACCA
    ATGGCTAAAACGACAGCAGAAGCTCAAAAAATGATAGATACAGCTAAATCAACAGGTAAA
    AAATTAACAATAGGTTATCAAAATCGTTTCCGAGCAGATAGTCAATTTTTACATCAAGCA
    GCGCAACGTGGCGACTTAGGAGACATTTACTTCGGAAAGGCACATGCCATTCGTCGTCGA
    GCAGTACCAACATGGGGTGTCTTTCTAGACGAAGAAGCTCAAGGTGGAGGACCATTAATC
    GATATCGGTACACACGCTTTAGATTTAACGTTATGGATGATGGATAATTATGAACCAGAA
    TCAGTGATGGGTTCAACATTCCATAAATTAAATAAACAGCATCATGCGGCAAACGCTTGG
    GGTTCATGGAATCCAGATGAATTTACAGTTGAAGATTCTGCGTTTGGATTTATTAAAATG
    AAGAATGGAGCGACGATCATTTTAGAATCCGCTTGGGCGATTAATTCTTTAGAAGTGGAT
    GAGGCAAAATGTTCATTATCAGGAACTAAAGCAGGTGCTGATATGAAAGATGGTCTACGT
    ATTCATGGTGAAGACATGGGTACACTTTATACCAAACACGTTGAATTGGAAAACAAAGGC
    GTCGACTTTTATGAAGGTAATGAAGTGGATGAAGCTGAAGAAGAAGCAAAAGCTTGGATT
    GATGCAGTTGTAAATGATACTGAACCAGTTGTGAAACCGGAACAAGCAATGGTAGTTACA
    AAAATTCTTGAAGCGATTTATCAGTCTGCAAAATCAGGCAAAGCAATTTACTTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0155 [new locus tag: NWMN_RS00845 ]
  • symbol: NWMN_0155
  • description: hypothetical protein
  • length: 359
  • theoretical pI: 4.90088
  • theoretical MW: 39311.2
  • GRAVY: -0.301393

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Sugars inositol 2-dehydrogenase (TIGR04380; EC 1.1.1.18; HMM-score: 117.7)
    and 3 more
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnB (TIGR03944; HMM-score: 18.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 14)
    Metabolism Central intermediary metabolism Nitrogen fixation homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 14)
  • TheSEED  :
    • Myo-inositol 2-dehydrogenase 1 (EC 1.1.1.18)
    Carbohydrates Sugar alcohols Inositol catabolism  Myo-inositol 2-dehydrogenase 1 (EC 1.1.1.18)
  • PFAM:
    NADP_Rossmann (CL0063) GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 109.1)
    and 6 more
    GADPH_aa-bio_dh (CL0139) GFO_IDH_MocA_C; Oxidoreductase family, C-terminal alpha/beta domain (PF02894; HMM-score: 71.5)
    GFO_IDH_MocA_C3; GFO/IDH/MocA C-terminal domain (PF22725; HMM-score: 65)
    NADP_Rossmann (CL0063) NAD_binding_3; Homoserine dehydrogenase, NAD binding domain (PF03447; HMM-score: 20.6)
    Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 14)
    Peptidase_SH (CL0201) DUF2213; Uncharacterized protein conserved in bacteria (DUF2213) (PF09979; HMM-score: 13.2)
    ATP_synthase (CL0255) V-ATPase_G_2; Vacuolar (H+)-ATPase G subunit (PF16999; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9791
    • Cytoplasmic Membrane Score: 0.0033
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0176
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025816
    • TAT(Tat/SPI): 0.001537
    • LIPO(Sec/SPII): 0.006777
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASAAEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAKTTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRAVPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANAWGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKDGLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQAMVVTKILEAIYQSAKSGKAIYFE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: MalR* (repression) regulon
    MalR*(TF)important in Maltose utilization, Maltodextrin utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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