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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0196 [new locus tag: SACOL_RS01000 ]
  • pan locus tag?: SAUPAN001069000
  • symbol: SACOL0196
  • pan gene symbol?: —
  • synonym:
  • product: Gfo/Idh/MocA family oxidoreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0196 [new locus tag: SACOL_RS01000 ]
  • symbol: SACOL0196
  • product: Gfo/Idh/MocA family oxidoreductase
  • replicon: chromosome
  • strand: +
  • coordinates: 229971..231047
  • length: 1077
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACGATTAAAGTTGGAATCATTGGGTGTGGTGGTATTGCGAATGGCAAGCACATGCCA
    AGTTTACAAAAAGTTGAAAATGTTGAAATGATCGCATTTTGTGACGTAGACATTTCGAAA
    GCAGCGAGTGCGGCAGAAGCATACGGAACTGACAATGCAAAGGTTTATGATGATTACAAA
    GCATTGTTAAAAGATGACACGATTGATGTTATCCATGTTTGTACGCCAAATGACTCGCAT
    TGTGAAATTACTGTAGCAGGGCTGCATGCTGGTAAACATGTGATGTGTGAAAAACCAATG
    GCTAAAACGACAGCAGAAGCTCAAAAAATGATAGATACAGCTAAATCAACAGGTAAAAAA
    TTAACAATAGGTTATCAAAATCGTTTCCGAGCAGATAGTCAATTTTTACATCAAGCAGCG
    CAACGTGGCGACTTAGGAGACATTTACTTCGGAAAGGCACATGCCATTCGTCGTCGAGCA
    GTACCAACATGGGGTGTCTTTCTAGACGAAGAAGCTCAAGGTGGAGGACCATTAATCGAT
    ATCGGTACACACGCTTTAGATTTAACGTTATGGATGATGGATAATTATGAACCAGAATCA
    GTGATGGGTTCAACATTCCATAAATTAAATAAACAGCATCATGCGGCAAACGCTTGGGGT
    TCATGGAATCCAGATGAATTTACAGTTGAAGATTCTGCGTTTGGATTTATTAAAATGAAG
    AATGGAGCGACGATCATTTTAGAATCCGCTTGGGCGATTAATTCTTTAGAAGTGGATGAG
    GCAAAATGTTCATTATCAGGAACTAAAGCAGGTGCTGATATGAAAGATGGTCTACGTATT
    CATGGTGAAGACATGGGTACACTTTATACCAAACACGTTGAATTGGAAAACAAAGGCGTC
    GACTTTTATGAAGGTAATGAAGTGGATGAAGCTGAAGAAGAAGCAAAAGCTTGGATTGAT
    GCAGTTGTAAATGATACTGAACCAGTTGTGAAACCGGAACAAGCAATGGTAGTTACAAAA
    ATTCTTGAAGCGATTTATCAGTCTGCAAAATCAGGCAAAGCAATTTACTTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1077

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0196 [new locus tag: SACOL_RS01000 ]
  • symbol: SACOL0196
  • description: Gfo/Idh/MocA family oxidoreductase
  • length: 358
  • theoretical pI: 4.90088
  • theoretical MW: 39180.1
  • GRAVY: -0.307542

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Sugars inositol 2-dehydrogenase (TIGR04380; EC 1.1.1.18; HMM-score: 117.7)
    and 3 more
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnB (TIGR03944; HMM-score: 18.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 14)
    Metabolism Central intermediary metabolism Nitrogen fixation homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 14)
  • TheSEED  :
    • Myo-inositol 2-dehydrogenase 1 (EC 1.1.1.18)
    Carbohydrates Sugar alcohols Inositol catabolism  Myo-inositol 2-dehydrogenase 1 (EC 1.1.1.18)
  • PFAM:
    NADP_Rossmann (CL0063) GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 102.8)
    and 4 more
    ox_reductase_C (CL0218) GFO_IDH_MocA_C; Oxidoreductase family, C-terminal alpha/beta domain (PF02894; HMM-score: 22.3)
    NADP_Rossmann (CL0063) NAD_binding_3; Homoserine dehydrogenase, NAD binding domain (PF03447; HMM-score: 19.1)
    Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 16.3)
    GT-B (CL0113) Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018816
    • TAT(Tat/SPI): 0.001064
    • LIPO(Sec/SPII): 0.003198
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASAAEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAKTTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRAVPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANAWGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKDGLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQAMVVTKILEAIYQSAKSGKAIYFE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: MalR (repression) regulon
    MalR(TF)important in Maltose utilization, Maltodextrin utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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