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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001311
  • pan locus tag?: SAUPAN003700000
  • symbol: JSNZ_001311
  • pan gene symbol?: hom
  • synonym:
  • product: homoserine dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001311
  • symbol: JSNZ_001311
  • product: homoserine dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1319225..1320505
  • length: 1281
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAAAAATTAAATATAGCATTATTAGGATTAGGTACTGTCGGATCTGGTGTTGTTAAA
    ATCATCGAAGAGAACCGACAGCAAATTCAAGATACATTAAATAAAGATATTGTCATAAAG
    CATATTCTTGTTCGAGATAAATCTAAAAAGAGACCGCTAAATATTAGCCAATATCATTTA
    ACTGAAGATGTTAATGAAATTTTAAATGATGATTCATTAGATATTATCGTTGAAGTCATG
    GGAGGAATTGAACCAACTGTAGATTGGTTAAGAACAGCACTTAAAAATAAAAAACATGTT
    ATTACCGCAAATAAAGATTTATTAGCAGTACATCTTAAACTTTTAGAAGATTTAGCAGAA
    GAAAATGGTGTAGCTTTAAAGTTTGAAGCGAGTGTAGCAGGTGGTATTCCGATCGTAAAT
    GCCATAAATAATGGTTTGAATGCGAATAATATTTCAAAATTTATGGGAATTTTAAATGGT
    ACCTCTAATTTTATTTTATCTAAAATGACTAAAGAGCAAACGACATTTGAGGAAGCACTT
    GATGAAGCGAAAAGACTTGGTTTTGCTGAAGCGGATCCAACTGATGATGTAGAAGGGGTA
    GATGCAGCGCGTAAAGTTGTCATTACATCATATTTATCATTTAACCAAGTCATTAAATTA
    AACGACGTTAAACGAAGAGGAATTAGTGGCGTAACTTTAACTGATATTAATGTAGCCGAT
    CAACTGGGGTATAAAATTAAATTGATTGGTAAGGGAATATATGAAAATGGCAAAGTTAAT
    GCATCGGTAGAACCAACGTTAATTGATAAAAAGCATCAATTAGCAGCTGTAGAGGATGAA
    TATAACGCGATTTATGTCATTGGTGATGCTGTTGGTGACACGATGTTTTATGGAAAAGGA
    GCAGGCAGTTTAGCAACAGGTAGTGCCGTTGTCAGTGATTTATTGAATGTAGCATTATTC
    TTTGAATCAGATTTACACACATTGCCACCACATTTTGAATTAAAGACAGATAAAACACGG
    GAAATGATGGATTCAGATGCAGAAATTAATATTAAAGAAAAATCCAATTTCTTTGTAGTA
    GTGAATCATGTCAAAGGTTCAATTGAAAATTTTGAAAATGAGTTAAAGGCAATATTACCA
    TTTCACCGATCATTAAGAGTTGCAAATTACGATAATCAATCATATGCCGCTGTTATAGTT
    GGATTGGAATCATCACCGGAAGAATTAATCACTAAGCATGGATACGAAGTTGACAAAGTA
    TACCCAGTAGAAGGAGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1281

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001311
  • symbol: JSNZ_001311
  • description: homoserine dehydrogenase
  • length: 426
  • theoretical pI: 5.04475
  • theoretical MW: 46873.2
  • GRAVY: -0.126291

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.3?  ExPASy
    Homoserine dehydrogenase L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H
  • TIGRFAM:
    Signal transduction Regulatory functions Protein interactions Phr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 12.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    GADPH_aa-bio_dh (CL0139) Homoserine_dh; Homoserine dehydrogenase (PF00742; HMM-score: 207.9)
    and 6 more
    no clan defined HSD_C; Homoserine dehydrogenase C-terminal domain (PF22101; HMM-score: 121.4)
    NADP_Rossmann (CL0063) NAD_binding_3; Homoserine dehydrogenase, NAD binding domain (PF03447; HMM-score: 70)
    no clan defined Img2; Mitochondrial large subunit ribosomal protein (Img2) (PF05046; HMM-score: 15.4)
    NADP_Rossmann (CL0063) Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 14.8)
    DXP_reductoisom; 1-deoxy-D-xylulose 5-phosphate reductoisomerase (PF02670; HMM-score: 14.6)
    HTH (CL0123) SKA1; Spindle and kinetochore-associated protein 1 (PF07160; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.987
    • Cytoplasmic Membrane Score: 0.006
    • Cell wall & surface Score: 0.0014
    • Extracellular Score: 0.0056
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.144791
    • TAT(Tat/SPI): 0.000632
    • LIPO(Sec/SPII): 0.040296
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILVRDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVITANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNGTSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVIKLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAAVEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFELKTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDNQSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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