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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001313
  • pan locus tag?: SAUPAN003702000
  • symbol: thrB
  • pan gene symbol?: thrB
  • synonym:
  • product: homoserine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001313
  • symbol: thrB
  • product: homoserine kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1321574..1322488
  • length: 915
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCGAATGTTTTGGAGTTAACAATTCCTGCATCAACAGCCAACCTTGGAGTTGGCTTT
    GATTCTATAGGTATGGCTTTAGATAAATTTTTGCATCTGTCTGTAAAGGAAACATCAGGG
    ACAAAATGGGAATATATTTTCCATGATGATGCATCTAAGCAATTGCCTACTGACGAAACA
    AACTTTATTTATCATGTAGCACAACAAGTTGCTTCTAAATATAGTGTTGACTTGCCTAAT
    TTATGTATCGAAATGAGAAGTGATATTCCATTGGCAAGAGGGTTAGGTTCGTCAGCTTCT
    GCTTTAGTAGGAGCTATATATATCGCAAATTATTTTGGTGATATCCAACTGTCTAAACAT
    GAGGTATTACAATTAGCGACTGAAATCGAAGGACATCCTGATAATGTTGCGCCGACCATT
    TATGGTGGTTTAATCGCTGGATATTATAATGATGTCTCGAAAGAAACGTCAGTTGCACAT
    ATCGACATACCAGACGTGGATGTGATTGTAACGATACCAACTTATGAACTAAAAACAGAA
    GCATCAAGACGTGCTTTACCACAAAAATTAACACATAGTGAAGCGGTTAAAAGTAGTGCA
    ATTAGTAATACAATGATTTGTGCATTAGCACAGCACAATTATGAATTAGCAGGTAAACTC
    ATGCAACAAGATGGCTTTCATGAACCGTATCGTCAGCATTTAATTGCTGAATTTGATGAA
    GTGAAAACAATTGCTAGTCAACATAATGCCTATGCAACTGTAATTAGTGGTGCTGGACCA
    ACTATTTTAATATTTAGTCGTAAAGAAAATAGTGGGGAATTGGTTCGCTCTTTAAATAGT
    CAGGTAGTATCATGCCATTCTGAATTGGTCGATATTAATATCAGTGGTGTTAAAGAACGA
    ATTGTATACCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001313
  • symbol: ThrB
  • description: homoserine kinase
  • length: 304
  • theoretical pI: 5.15013
  • theoretical MW: 33266.4
  • GRAVY: -0.0641447

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.39?  ExPASy
    Homoserine kinase ATP + L-homoserine = ADP + O-phospho-L-homoserine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 260.2)
    and 6 more
    Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 47)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 29.9)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 28.8)
    Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 22.9)
    Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 16.9)
    Metabolism Energy metabolism Sugars galactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 12.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 68.8)
    and 3 more
    GHMP_C (CL0677) GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 38.4)
    S5 (CL0329) MVD-like_N; Diphosphomevalonate decarboxylase-like N-terminal domain (PF22700; HMM-score: 19.6)
    no clan defined DUF3963; Protein of unknown function (DUF3963) (PF13124; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.981
    • Cytoplasmic Membrane Score: 0.014
    • Cell wall & surface Score: 0.0011
    • Extracellular Score: 0.0039
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013793
    • TAT(Tat/SPI): 0.00101
    • LIPO(Sec/SPII): 0.001581
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWEYIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASALVGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAHIDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAGKLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVRSLNSQVVSCHSELVDINISGVKERIVYQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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