PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1228 [new locus tag: SAUSA300_RS06655 ]
• pan locus tag^?: SAUPAN003702000
• symbol: thrB
• pan gene symbol^?: thrB
• synonym:
• product: homoserine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1228 [new locus tag: SAUSA300_RS06655 ]
• symbol: thrB
• product: homoserine kinase
• replicon: chromosome
• strand: +
• coordinates: 1345739..1346653
• length: 915
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914823 NCBI
• RefSeq: YP_493925 NCBI
• BioCyc: see SAUSA300_RS06655
• MicrobesOnline: 1292743 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTCGAATGTTTTGGAGTTAACAATTCCTGCATCAACAGCCAACCTTGGAGTTGGCTTT
  GATTCTATAGGTATGGCTTTAGATAAATTTTTGCATCTGTCTGTAAAGGAAACATCAGGG
  ACAAAATGGGAATATATTTTCCATGATGATGCATCTAAGCAATTGCCTACTGACGAAACA
  AACTTTATTTATCATGTAGCACAACAAGTTGCTTCTAAATATAGTGTTGACTTGCCTAAT
  TTATGTATCGAAATGAGAAGTGATATTCCATTGGCAAGAGGGTTAGGTTCGTCAGCTTCT
  GCTTTAGTAGGAGCTATATATATCGCAAATTATTTTGGTGATATCCAACTGTCTAAACAT
  GAGGTATTACAATTAGCGACTGAAATCGAAGGACATCCTGATAATGTTGCGCCGACCATT
  TATGGTGGTTTAATCGCTGGATATTATAATGATGTCTCGAAAGAAACGTCAGTTGCACAT
  ATCGACATACCAGACGTGGATGTGATTGTAACGATACCAACTTATGAACTAAAAACAGAA
  GCATCAAGACGTGCTTTACCACAAAAATTAACACATAGTGAAGCGGTTAAAAGTAGTGCA
  ATTAGTAATACAATGATTTGTGCATTAGCACAGCACAATTATGAATTAGCAGGTAAACTC
  ATGCAACAAGATGGCTTTCATGAACCGTATCGTCAGCATTTAATTGCTGAATTTGATGAA
  GTGAAAACAATTGCTAGTCAACATAATGCCTATGCAACTGTAATTAGTGGTGCTGGACCA
  ACTATTTTAATATTTAGTCGTAAAGAAAATAGTGGGGAATTGGTTCGCTCTTTAAATAGT
  CAGGTAGTATCATGCCATTCTGAATTGGTCGATATTAATATCAGTGGTGTTAAAGAACGA
  ATTGTATACCAATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1228 [new locus tag: SAUSA300_RS06655 ]
• symbol: ThrB
• description: homoserine kinase
• length: 304
• theoretical pI: 5.15013
• theoretical MW: 33266.4
• GRAVY: -0.0641447

⊟Function

• reaction:
  EC 2.7.1.39?  ExPASy
  Homoserine kinase ATP + L-homoserine = ADP + O-phospho-L-homoserine
• TIGRFAM:
  Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 260.2)
  and 6 more
  Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 47)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 29.9)
  Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 28.8)
  Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 22.9)
  Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 16.9)
  Metabolism Energy metabolism Sugars galactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 12.5)
• TheSEED  :
  • Homoserine kinase (EC 2.7.1.39)
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Homoserine kinase (EC 2.7.1.39)
  and 1 more
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine and Homoserine Biosynthesis  Homoserine kinase (EC 2.7.1.39)
• PFAM:
  S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 52.9)
  and 2 more
  no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 34.9)
  DUF3963; Protein of unknown function (DUF3963) (PF13124; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.013793
  • TAT(Tat/SPI): 0.00101
  • LIPO(Sec/SPII): 0.001581
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159971 NCBI
• RefSeq: YP_493925 NCBI
• UniProt: Q2FHA3 UniProt

⊟Protein sequence

• MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWEYIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASALVGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAHIDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAGKLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVRSLNSQVVSCHSELVDINISGVKERIVYQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1226 > thrC > thrB > SAUSA300_1229

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications