PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1227 [new locus tag: SAUSA300_RS06650 ]
• pan locus tag^?: SAUPAN003701000
• symbol: thrC
• pan gene symbol^?: thrC
• synonym:
• product: threonine synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1227 [new locus tag: SAUSA300_RS06650 ]
• symbol: thrC
• product: threonine synthase
• replicon: chromosome
• strand: +
• coordinates: 1344676..1345737
• length: 1062
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915053 NCBI
• RefSeq: YP_493924 NCBI
• BioCyc: see SAUSA300_RS06650
• MicrobesOnline: 1292742 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  1021
  
  ATGAGAAGATGGCAAGGATTAGTAGAAGAGTTTAAAGCACATTTACCAGTAAATGAAAAT
  ACACCAAAATTAACATTGAACGAGGGAAATACACCACTCATTCATTGTGAAAATATGTCT
  AAAATACTAGGCATAGATTTATATGTGAAGTATGAAGGTGCCAATCCGACAGGTTCATTT
  AAAGATCGCGGTATGGTAATGGCTGTGACAAAAGCAAAAGAGCAAGGTAAGAAAATTGTA
  ATATGCGCTTCGACTGGAAATACATCAGCGTCTGCAGCAGCATATGCAGCGAGAGCAGGT
  TTAAAAGCTATCGTCGTAATACCAGAAGGTAAAATTGCATTAGGTAAATTGTCGCAAGCA
  GTAATGTATGGTGCAGAAATCGTTTCTATTGAAGGAAACTTTGATGAAGCTTTAGAAATT
  GTAAAAGAAATTGCAAAAAGTGGCGAAATCGAGCTTGTAAACTCTGTCAATCCATTTAGA
  ATCGAAGGACAAAAGACAGGCTCATTTGAAATTGTACAACAATTAGACGGTGAAGCACCT
  GATATTTTAGCGATTCCTGTAGGTAATGCAGGTAATATTACTGCATATTGGAAAGGCTTT
  AAAGAATATCATGAAGCTAAAGGATCACAATTGCCGAAAATGTTTGGCTTCCAAGCTGAA
  GGCGCATCACCAATTGTTCAAAATAAAGTCATTAAAAATCCTGAAACGATTGCAACTGCT
  ATTCGAATTGGTAATCCTGCTAGTTGGGATAAGGCGACTAATGCTCTTAAAGAATCAAAT
  GGATTAATAGATAGTGTTACTGATGATGAAATTCTAGAAGCATATCAGTTAATGACAACT
  AAAGAAGGTGTCTTTAGTGAACCAGCGAGTAATGCTTCTATTGCAGGTTTAATTAAATTG
  CATAGACAAGGTAAATTACCTCAAGGTAAAAAAGTAGTTGCTATTTTAACTGGTAATGGA
  TTAAAAGATCCTGATACTGCTATTTCACTACTAGATAATCCGATAAAGCCATTGCCAAAT
  GATAAAGATAGCATTATCGATTATATTAAAGGAGCTTTATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1227 [new locus tag: SAUSA300_RS06650 ]
• symbol: ThrC
• description: threonine synthase
• length: 353
• theoretical pI: 6.1777
• theoretical MW: 37869.2
• GRAVY: -0.172805

⊟Function

• reaction:
  EC 4.2.3.1?  ExPASy
  Threonine synthase O-phospho-L-homoserine + H₂O = L-threonine + phosphate
• TIGRFAM:
  Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 352.3)
  and 17 more
  Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 105.6)
  Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 85.8)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 78.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 78.7)
  Metabolism Energy metabolism Methanogenesis cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 78.7)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 77)
  Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 72.7)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 69.4)
  ectoine utilization protein EutB (TIGR02991; HMM-score: 60.3)
  Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 54.2)
  Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 52.3)
  Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 48.5)
  Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 36.8)
  Metabolism Central intermediary metabolism Other 1-aminocyclopropane-1-carboxylate deaminase (TIGR01274; EC 3.5.99.7; HMM-score: 29.8)
  pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 26.1)
  Metabolism Energy metabolism Other diaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 23)
  diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 20.1)
• TheSEED  :
  • Threonine synthase (EC 4.2.3.1)
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine and Homoserine Biosynthesis  Threonine synthase (EC 4.2.3.1)
• PFAM:
  NADP_Rossmann (CL0063) PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 267.9)
  and 4 more
  Phosphatase (CL0031) Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 13.4)
  no clan defined PrpR_N; Propionate catabolism activator (PF06506; HMM-score: 13.2)
  DUF4687; Domain of unknown function (DUF4687) (PF15747; HMM-score: 12.6)
  Leu-IlvD (CL0364) PA; PA domain (PF02225; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9766
  • Cytoplasmic Membrane Score: 0.0061
  • Cell wall & surface Score: 0.0007
  • Extracellular Score: 0.0166
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008553
  • TAT(Tat/SPI): 0.000483
  • LIPO(Sec/SPII): 0.000737
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160676 NCBI
• RefSeq: YP_493924 NCBI
• UniProt: A0A0H2XH24 UniProt

⊟Protein sequence

• MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILGIDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLKAIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFRIEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQAEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQLMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNPIKPLPNDKDSIIDYIKGAL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1226 > thrC > thrB > SAUSA300_1229

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications