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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1362 [new locus tag: SACOL_RS06930 ]
  • pan locus tag?: SAUPAN003700000
  • symbol: hom
  • pan gene symbol?: hom
  • synonym:
  • product: homoserine dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1362 [new locus tag: SACOL_RS06930 ]
  • symbol: hom
  • product: homoserine dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1367193..1368473
  • length: 1281
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAAAAATTAAATATAGCATTATTAGGATTAGGTACTGTCGGATCTGGTGTTGTTAAA
    ATCATCGAAGAGAACCGACAGCAAATTCAAGATACATTAAATAAAGATATTGTCATAAAG
    CATATTCTTGTTCGAGATAAATCTAAAAAGAGACCGCTAAATATTAGCCAATATCATTTA
    ACTGAAGATGTTAATGAAATTTTAAATGATGATTCATTAGATATTATCGTTGAAGTCATG
    GGAGGAATTGAACCAACTGTAGATTGGTTAAGAACAGCACTTAAAAATAAAAAACATGTT
    ATTACCGCAAATAAAGATTTATTAGCAGTACATCTTAAACTTTTAGAAGATTTAGCAGAA
    GAAAATGGTGTAGCTTTAAAGTTTGAAGCGAGTGTAGCAGGTGGTATTCCGATCGTAAAT
    GCCATAAATAATGGTTTGAATGCGAATAATATTTCAAAATTTATGGGAATTTTAAATGGT
    ACCTCTAATTTTATTTTATCTAAAATGACTAAAGAGCAAACGACATTTGAGGAAGCACTT
    GATGAAGCGAAAAGACTTGGTTTTGCTGAAGCGGATCCAACTGATGATGTAGAAGGGGTA
    GATGCAGCGCGTAAAGTTGTCATTACATCATATTTATCATTTAACCAAGTCATTAAATTA
    AACGACGTTAAACGAAGAGGAATTAGTGGCGTAACTTTAACTGATATTAATGTAGCCGAT
    CAACTGGGGTATAAAATTAAATTGATTGGTAAGGGAATATATGAAAATGGCAAAGTTAAT
    GCATCGGTAGAACCAACGTTAATTGATAAAAAGCATCAATTAGCAGCTGTAGAGGATGAA
    TATAACGCGATTTATGTTATTGGTGATGCCGTTGGTGACACGATGTTTTATGGAAAAGGA
    GCAGGCAGTTTAGCAACAGGTAGTGCCGTTGTCAGTGATTTATTGAATGTAGCATTATTC
    TTTGAATCAGATTTACACACATTGCCACCACATTTTGAATTAAAGACAGATAAAACACGG
    GAAATGATGGATTCAGATGCAGAAATTAATATTAAAGAAAAATCCAATTTCTTTGTAGTA
    GTGAATCATGTCAAAGGTTCAATTGAAAATTTTGAAAATGAGTTAAAGGCAATATTACCA
    TTTCACCGATCATTAAGAGTTGCAAATTACGATAATCAATCATATGCCGCTGTTATAGTT
    GGATTGGAATCATCACCGGAAGAATTAATCACTAAGCATGGATACGAAGTTGACAAAGTA
    TACCCAGTAGAAGGAGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1281

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1362 [new locus tag: SACOL_RS06930 ]
  • symbol: Hom
  • description: homoserine dehydrogenase
  • length: 426
  • theoretical pI: 5.04475
  • theoretical MW: 46873.2
  • GRAVY: -0.126291

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.3?  ExPASy
    Homoserine dehydrogenase L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H
  • TIGRFAM:
    Signal transduction Regulatory functions Protein interactions Phr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 12.4)
  • TheSEED  :
    • Homoserine dehydrogenase (EC 1.1.1.3)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Homoserine dehydrogenase (EC 1.1.1.3)
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine and Homoserine Biosynthesis  Homoserine dehydrogenase (EC 1.1.1.3)
  • PFAM:
    no clan defined Homoserine_dh; Homoserine dehydrogenase (PF00742; HMM-score: 201.5)
    and 5 more
    NADP_Rossmann (CL0063) NAD_binding_3; Homoserine dehydrogenase, NAD binding domain (PF03447; HMM-score: 65.9)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 14)
    DXP_reductoisom; 1-deoxy-D-xylulose 5-phosphate reductoisomerase (PF02670; HMM-score: 13.6)
    no clan defined Img2; Mitochondrial large subunit ribosomal protein (Img2) (PF05046; HMM-score: 13.3)
    HAD (CL0137) Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.144791
    • TAT(Tat/SPI): 0.000632
    • LIPO(Sec/SPII): 0.040296
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILVRDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVITANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNGTSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVIKLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAAVEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFELKTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDNQSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 421 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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