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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001271
  • pan locus tag?: SAUPAN003612000
  • symbol: glpK
  • pan gene symbol?: glpK
  • synonym:
  • product: glycerol kinase GlpK

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001271
  • symbol: glpK
  • product: glycerol kinase GlpK
  • replicon: chromosome
  • strand: +
  • coordinates: 1287886..1289382
  • length: 1497
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAAAATATATTTTATCTATAGACCAAGGAACAACAAGCTCAAGAGCGATTTTATTC
    AATCAAAAAGGGGAAATTGCAGGGGTAGCACAACGTGAGTTTAAGCAATATTTTCCACAA
    TCAGGTTGGGTTGAACATGATGCAAATGAAATTTGGACATCTGTGTTAGCTGTAATGACG
    GAAGTAATTAATGAAAATGATGTTAGAGCTGATCAAATTGCAGGTATCGGTATTACAAAC
    CAACGTGAAACAACGGTTGTTTGGGACAAACATACTGGCCGCCCAATTTATCACGCAATT
    GTTTGGCAATCACGTCAAACACAATCAATTTGTTCAGAATTAAAACAACAAGGATATGAA
    CAAACATTTAGAGATAAGACAGGATTACTTTTAGATCCGTATTTTGCAGGTACAAAAGTT
    AAATGGATTCTAGACAATGTTGAAGGTGCACGAGAAAAAGCAGAAAATGGCGATCTATTA
    TTTGGAACGATTGATACTTGGTTAGTATGGAAATTATCAGGAAAAGCTGCTCATATTACT
    GATTATTCAAATGCGAGTCGTACATTAATGTTTAATATCCATGATTTAGAATGGGACGAT
    GAGTTATTAGAACTACTTACAGTACCTAAAAATATGTTGCCAGAAGTTAAAGCTTCGAGT
    GAAGTATATGGTAAGACAATTGATTACCACTTCTATGGTCAAGAAGTACCAATCGCTGGA
    GTAGCTGGTGATCAACAAGCAGCATTATTTGGACAAGCTTGCTTCGAACGTGGTGACGTG
    AAAAACACATATGGAACTGGTGGCTTCATGTTAATGAATACAGGTGACAAAGCGGTTAAA
    TCTGAAAGTGGTTTATTAACAACAATTGCTTATGGTATTGATGGAAAAGTAAATTATGCG
    CTTGAAGGTTCCATCTTTGTTTCGGGTTCAGCAATCCAATGGTTACGTGATGGATTAAGA
    ATGATTAATTCAGCACCACAATCAGAAAGTTATGCGACACGAGTTGACTCTACTGAGGGT
    GTCTATGTTGTTCCAGCTTTTGTAGGTTTAGGAACACCATATTGGGATTCTGAAGCACGT
    GGTGCGATTTTCGGTTTAACACGTGGAACTGAAAAAGAGCACTTTATCCGTGCAACTTTA
    GAATCATTATGTTACCAAACTCGTGACGTTATGGAAGCAATGTCAAAAGACTCTGGTATT
    GATGTCCAAAGTTTACGTGTCGATGGTGGTGCAGTTAAAAATAACTTTATTATGCAGTTC
    CAAGCAGACATTGTTAATACTTCTGTTGAAAGACCTGAAATTCAAGAAACTACAGCTTTA
    GGTGCTGCATTTTTAGCAGGATTAGCAGTTGGATTCTGGGAGAGTAAAGATGATATCGCT
    AAAAACTGGAAATTAGAAGAAAAATTCGATCCGAAAATGGATGAAGGCGAAAGAGAAAAA
    TTATATAGAGGTTGGAAAAAAGCTGTTGAAGCAACACAAGTTTTTAAAACAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1497

⊟Protein[edit | edit source]

Protein Data Bank: 3G25
Protein Data Bank: 3GE1

⊟General[edit | edit source]

  • locus tag: JSNZ_001271
  • symbol: GlpK
  • description: glycerol kinase GlpK
  • length: 498
  • theoretical pI: 4.6683
  • theoretical MW: 55625.2
  • GRAVY: -0.340964

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.30?  ExPASy
    Glycerol kinase ATP + glycerol = ADP + sn-glycerol 3-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Other glycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 760.2)
    and 7 more
    Metabolism Energy metabolism Sugars xylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 240.7)
    Metabolism Energy metabolism Sugars gluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 173.5)
    Metabolism Energy metabolism Sugars L-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 120.9)
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 109.4)
    Metabolism Energy metabolism Sugars ribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 68.5)
    rhamnulokinase (TIGR02627; EC 2.7.1.5; HMM-score: 57.7)
    TraF-like protein (TIGR02740; HMM-score: 12.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Actin_ATPase (CL0108) FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 297)
    and 2 more
    FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 165.5)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 19)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9394
    • Cytoplasmic Membrane Score: 0.0121
    • Cell wall & surface Score: 0.0013
    • Extracellular Score: 0.0472
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004055
    • TAT(Tat/SPI): 0.000391
    • LIPO(Sec/SPII): 0.000446
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLLFGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKASSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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