PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001271
• pan locus tag^?: SAUPAN003612000
• symbol: glpK
• pan gene symbol^?: glpK
• synonym:
• product: glycerol kinase GlpK

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001271
• symbol: glpK
• product: glycerol kinase GlpK
• replicon: chromosome
• strand: +
• coordinates: 1287886..1289382
• length: 1497
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGGAAAAATATATTTTATCTATAGACCAAGGAACAACAAGCTCAAGAGCGATTTTATTC
  AATCAAAAAGGGGAAATTGCAGGGGTAGCACAACGTGAGTTTAAGCAATATTTTCCACAA
  TCAGGTTGGGTTGAACATGATGCAAATGAAATTTGGACATCTGTGTTAGCTGTAATGACG
  GAAGTAATTAATGAAAATGATGTTAGAGCTGATCAAATTGCAGGTATCGGTATTACAAAC
  CAACGTGAAACAACGGTTGTTTGGGACAAACATACTGGCCGCCCAATTTATCACGCAATT
  GTTTGGCAATCACGTCAAACACAATCAATTTGTTCAGAATTAAAACAACAAGGATATGAA
  CAAACATTTAGAGATAAGACAGGATTACTTTTAGATCCGTATTTTGCAGGTACAAAAGTT
  AAATGGATTCTAGACAATGTTGAAGGTGCACGAGAAAAAGCAGAAAATGGCGATCTATTA
  TTTGGAACGATTGATACTTGGTTAGTATGGAAATTATCAGGAAAAGCTGCTCATATTACT
  GATTATTCAAATGCGAGTCGTACATTAATGTTTAATATCCATGATTTAGAATGGGACGAT
  GAGTTATTAGAACTACTTACAGTACCTAAAAATATGTTGCCAGAAGTTAAAGCTTCGAGT
  GAAGTATATGGTAAGACAATTGATTACCACTTCTATGGTCAAGAAGTACCAATCGCTGGA
  GTAGCTGGTGATCAACAAGCAGCATTATTTGGACAAGCTTGCTTCGAACGTGGTGACGTG
  AAAAACACATATGGAACTGGTGGCTTCATGTTAATGAATACAGGTGACAAAGCGGTTAAA
  TCTGAAAGTGGTTTATTAACAACAATTGCTTATGGTATTGATGGAAAAGTAAATTATGCG
  CTTGAAGGTTCCATCTTTGTTTCGGGTTCAGCAATCCAATGGTTACGTGATGGATTAAGA
  ATGATTAATTCAGCACCACAATCAGAAAGTTATGCGACACGAGTTGACTCTACTGAGGGT
  GTCTATGTTGTTCCAGCTTTTGTAGGTTTAGGAACACCATATTGGGATTCTGAAGCACGT
  GGTGCGATTTTCGGTTTAACACGTGGAACTGAAAAAGAGCACTTTATCCGTGCAACTTTA
  GAATCATTATGTTACCAAACTCGTGACGTTATGGAAGCAATGTCAAAAGACTCTGGTATT
  GATGTCCAAAGTTTACGTGTCGATGGTGGTGCAGTTAAAAATAACTTTATTATGCAGTTC
  CAAGCAGACATTGTTAATACTTCTGTTGAAAGACCTGAAATTCAAGAAACTACAGCTTTA
  GGTGCTGCATTTTTAGCAGGATTAGCAGTTGGATTCTGGGAGAGTAAAGATGATATCGCT
  AAAAACTGGAAATTAGAAGAAAAATTCGATCCGAAAATGGATGAAGGCGAAAGAGAAAAA
  TTATATAGAGGTTGGAAAAAAGCTGTTGAAGCAACACAAGTTTTTAAAACAGAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1497
  

⊟Protein

• 3G25
  PDB
• 3GE1
  PDB

Protein Data Bank: 3G25

Protein Data Bank: 3GE1

⊟General

• locus tag: JSNZ_001271
• symbol: GlpK
• description: glycerol kinase GlpK
• length: 498
• theoretical pI: 4.6683
• theoretical MW: 55625.2
• GRAVY: -0.340964

⊟Function

• reaction:
  EC 2.7.1.30?  ExPASy
  Glycerol kinase ATP + glycerol = ADP + sn-glycerol 3-phosphate
• TIGRFAM:
  Metabolism Energy metabolism Other glycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 760.2)
  and 7 more
  Metabolism Energy metabolism Sugars xylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 240.7)
  Metabolism Energy metabolism Sugars gluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 173.5)
  Metabolism Energy metabolism Sugars L-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 120.9)
  FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 109.4)
  Metabolism Energy metabolism Sugars ribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 68.5)
  rhamnulokinase (TIGR02627; EC 2.7.1.5; HMM-score: 57.7)
  TraF-like protein (TIGR02740; HMM-score: 12.8)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Actin_ATPase (CL0108) FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 297)
  and 2 more
  FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 165.5)
  BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 19)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9394
  • Cytoplasmic Membrane Score: 0.0121
  • Cell wall & surface Score: 0.0013
  • Extracellular Score: 0.0472
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004055
  • TAT(Tat/SPI): 0.000391
  • LIPO(Sec/SPII): 0.000446
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLLFGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKASSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications