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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001272
  • pan locus tag?: SAUPAN003613000
  • symbol: JSNZ_001272
  • pan gene symbol?: glpD
  • synonym:
  • product: glycerol-3-phosphate dehydrogenase/oxidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001272
  • symbol: JSNZ_001272
  • product: glycerol-3-phosphate dehydrogenase/oxidase
  • replicon: chromosome
  • strand: +
  • coordinates: 1289540..1291213
  • length: 1674
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCATTGTCTACTTTTAAGAGAGAACATATTAAAAAGAATTTAAGAAATGATGAATAT
    GATTTAGTAATTATTGGTGGCGGTATTACAGGTGCAGGTATTGCACTAGACGCGAGTGAA
    AGAGGAATGAAAGTTGCATTAGTTGAAATGCAAGACTTTGCACAAGGAACAAGCTCAAGA
    TCTACAAAATTAGTCCATGGTGGTTTACGTTACTTAAAACAATTCCAAATTGGAGTAGTT
    GCCGAAACTGGTAAAGAACGTGCGATTGTTTATGAAAATGGACCTCATGTTACGACTCCA
    GAGTGGATGCTTTTACCAATGCATAAAGGTGGAACATTTGGTAAATTCTCAACATCAATT
    GGTTTAGGAATGTATGATCGTTTAGCAGGTGTTAAGAAGTCTGAACGTAAAAAAATGTTA
    TCTAAAAAAGAAACTTTAGCTAAAGAACCATTAGTTAAAAAAGAAGGTCTAAAAGGCGGC
    GGTTACTATGTTGAATATCGTACTGACGATGCGCGTTTAACTATTGAAGTTATGAAGCGT
    GCTGCTGAAAAAGGCGCAGAAATTATCAACTATACTAAATCTGAACACTTCACTTATGAT
    AAAAATCAACAAGTAAATGGTGTTAAAGTTATAGATAAATTAACTAATGAAAATTATACA
    ATTAAGGCTAAAAAAGTGGTTAATGCAGCAGGTCCATGGGTTGATGATGTTAGAAGTGGT
    GATTATGCACGCAATAATAAAAAATTACGTTTAACTAAAGGTGTACATGTTGTTATTGAT
    CAATCAAAATTCCCATTAGGTCAAGCAGTATACTTTGATACTGAAAAAGATGGAAGAATG
    ATTTTTGCAATTCCACGTGAAGGAAAAGCGTATGTAGGTACTACAGATACATTCTATGAC
    AATATCAAATCTTCACCATTAACTACACAAGAAGACAGAGACTATTTAATCGATGCGATT
    AATTACATGTTCCCTAGTGTTAATGTTACAGATGAAGATATTGAATCAACATGGGCAGGA
    ATTAGACCATTAATTTACGAAGAAGGCAAAGACCCTTCTGAAATCTCTCGTAAGGATGAA
    ATTTGGGAAGGTAAATCAGGTTTATTAACTATTGCAGGTGGTAAATTAACAGGCTATCGT
    CACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTA
    ACATTTAGTCCATGTAATACAAAAGGTCTGGCAATTTCAGGTGGCGATGTAGGTGGTAGC
    AAGAACTTTGATGCGTTTGTAGAGCAAAAAGTAGATGTAGCTAAAGGATTCGGCATTGAT
    GAAGATGTTGCAAGACGTTTAGCATCTAAATATGGTTCAAATGTTGATGAATTGTTCAAC
    ATTGCGCAAACATCTCAATACCATGATAGCAAGTTACCATTAGAAATTTATGTAGAACTT
    GTTTATAGTATTCAACAAGAAATGGTATACAAACCTAACGATTTCTTAGTTCGTCGTTCT
    GGTAAAATGTATTTCAATATTAAAGATGTATTAGATTATAAAGATGCTGTCATCGATATT
    ATGGCAGATATGCTTGATTACTCTCCAGCTCAAATTGAAGCATATACTGAAGAAGTTGAG
    CAAGCAATTAAAGAAGCGCAACATGGAAATAATCAACCAGCAGTTAAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1674

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001272
  • symbol: JSNZ_001272
  • description: glycerol-3-phosphate dehydrogenase/oxidase
  • length: 557
  • theoretical pI: 7.03905
  • theoretical MW: 62387.5
  • GRAVY: -0.490844

⊟Function[edit | edit source]

  • reaction:
    EC 1.-.-.-?  ExPASy
  • TIGRFAM:
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, A subunit (TIGR03377; EC 1.1.5.3; HMM-score: 175.5)
    and 26 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 48.2)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 33.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 31.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 27.4)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 23.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 22)
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 19.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 18.9)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 18.3)
    lycopene cyclase family protein (TIGR01790; HMM-score: 18)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 16.1)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 16.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 14.7)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 14.6)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, monomeric form (TIGR01377; HMM-score: 14.2)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 13.5)
    putative aminophosphonate oxidoreductase (TIGR03329; HMM-score: 13.2)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, beta subunit family (TIGR01373; HMM-score: 12.3)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.2)
    Metabolism Energy metabolism Anaerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.2)
    Metabolism Energy metabolism Aerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.2)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase, flavoprotein subunit (TIGR01816; HMM-score: 11.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 11.6)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 11.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other C-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 10.2)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 9.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 205.5)
    and 13 more
    Fer2_BFD (CL0667) DAO_C; C-terminal domain of alpha-glycerophosphate oxidase (PF16901; HMM-score: 86.9)
    NADP_Rossmann (CL0063) FAD_binding_2; FAD binding domain (PF00890; HMM-score: 25.8)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 24.7)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 24.7)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 22.4)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 20.7)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 20.1)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 17.5)
    GMC_oxred_N; GMC oxidoreductase (PF00732; HMM-score: 15.9)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 15.5)
    Thi4; Thi4 family (PF01946; HMM-score: 15.1)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 13.3)
    HI0933_like; HI0933-like protein Rossmann domain (PF03486; HMM-score: 10.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.6962
    • Cytoplasmic Membrane Score: 0.2967
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0069
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007739
    • TAT(Tat/SPI): 0.004601
    • LIPO(Sec/SPII): 0.0012
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMKVALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEWMLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGGGYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNENYTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEKDGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDIESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSKRLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASKYGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIKDVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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