PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001017
• pan locus tag^?: SAUPAN003260000
• symbol: atl
• pan gene symbol^?: atl
• synonym: atlA
• product: bifunctional autolysin

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001017
• symbol: atl
• product: bifunctional autolysin
• replicon: chromosome
• strand: -
• coordinates: 1018073..1021834
• length: 3762
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  2341
  2401
  2461
  2521
  2581
  2641
  2701
  2761
  2821
  2881
  2941
  3001
  3061
  3121
  3181
  3241
  3301
  3361
  3421
  3481
  3541
  3601
  3661
  3721
  
  ATGGCGAAAAAATTCAATTACAAACTACCATCAATGGTTGCATTAACGCTTGTAGGTTCA
  GCAGTCACTGCACATCAAGTTCAAGCAGCTGAGACGACACAAGATCAAACTACTAATAAA
  AACGTTTTAGATAGTAATAAAGTTAAAGCAACTACTGAACAAGCAAAGGCTGAGGTAAAA
  AATCCAACGCAAAACATTTCTGGCACTCAAGTATATCAAGACCCTGCTATTGTCCAACCA
  AAAACAGCAAATAACAAAACAGGCAATGCTCAAGTAAGTCAAAAAGTTGATACTGCACAA
  GTAAATGGTGACACTCGTGCTAATCAATCAGCGACTACAAATAATACGCAGCCTGTTGCA
  AAGTCAACAAGCACTACAGCACCTAAAACTAACACTAATGTTACAAATGCTGGTTATAGT
  TTAGTTGATGATGAAGATGATAATTCAGAAAATCAAATTAATCCAGAATTAATTAAATCA
  GCTGCTAAACCTGCAGCTCTTGAAACGCAATATAAAGCCGCAGCACCTAAAGCTGCAACT
  ACAACAGCACCTAAAGCTAAAACTGAAGCGACACCTAAAGTAACTACTTTTAGCGCTTCA
  GCACAACCAAGATCAGTTGCTGCAACACCAAAAACGAGTTTGCCAAAATATAAACCACAA
  GTAAACTCTTCAATTAACGATTACATTCGTAAAAATAACTTAAAAGCACCTAAGATTGAA
  GAAGATTATACATCTTACTTCCCTAAATACGCATACCGTAACGGTGTTGGGCGTCCTGAA
  GGTATCGTAGTTCATGATACAGCTAATGATCGTTCGACGATAAATGGTGAAATTAGTTAT
  ATGAAAAACAACTATCAAAACGCATTCGTACATGCATTTGTTGATGGGGATCGTATAATC
  GAAACAGCACCAACGGATTACTTATCTTGGGGTGTCGGTGCAGTCGGTAACCCTAGATTC
  ATCAATGTTGAAATCGTTCATACACACGATTATGCTTCATTTGCACGTTCAATGAATAAC
  TATGCTGACTATGCAGCTACACAATTGCAATATTATGGTTTGAAACCTGATAGTGCTGAA
  TATGATGGAAATGGTACAGTATGGACTCACTACGCTGTAAGTAAATATTTAGGTGGTACG
  GACCATGCCGATCCACATGGATATTTAAGAAGTCATAATTATAGTTATGATCAATTATAT
  GACTTAATTAATGAAAAATATTTAATAAAAATGGGTAAAGTGGCGCCATGGGGTACGCAA
  TCTACAACTACCCCTACTACACCGTCGAAACCATCAACACCATCAACTGGTAAATTAACA
  GTTGCTGCTAATAATGGTGTCGCTCAAATTAAACCGACAAATAGTGGCTTATATACTACT
  GTTTACGACAAAACTGGTAAAGCAACTAATGAAGTTCAAAAAACATTTGCTGTATCTAAA
  ACAGCTACATTAGGTAATCAAAAATTCTATCTTGTTCAAGATTACAATTCTGGTAATAAA
  TTTGGTTGGGTTAAAGAAGGCGATGTGGTTTACAACACAGCTAAATCACCTGTAAATGTA
  AATCAATCATATTCAATCAAACCTGGTACGAAACTTTATACAGTACCTTGGGGTACATCT
  AAACAAGTTGCTGGTAGCGTGTCTGGATCTGGAAACCAAACATTTAAAGCTTCAAAGCAA
  CAACAAATTGATAAATCAATTTATTTATATGGCTCTGTGAATGGTAAATCTGGTTGGGTA
  AGTAAAGCATATTTAGTTGATACTGCTAAACCTACGCCTACACCAACACCTAAGCCATCA
  ACACCTACAACAAATAATAAATTAACAGTTTCATCATTAAACGGTGTTGCTCAAATTAAT
  GCTAAAAACAATGGCTTATTCACTACAGTTTATGACAAAACTGGTAAGCCAACGAAAGAA
  GTTCAAAAAACATTTGCTGTAACAAAAGAAGCAAGTCTAGGTGGAAACAAATTCTACTTA
  GTTAAAGATTACAATAGTCCAACTTTAATTGGTTGGGTTAAACAAGGTGACGTTATTTAT
  AACAATGCAAAATCACCTGTAAATGTAATGCAAACATATACAGTAAAACCAGGCACTAAA
  TTATATTCAGTACCTTGGGGTACTTATAAACAAGAAGCTGGTGCGGTATCTGGTACAGGT
  AACCAAACTTTTAAAGCGACTAAGCAACAACAAATTGATAAATCTATCTATTTATATGGA
  ACTGTAAATGGTAAATCCGGTTGGGTAAGTAAAGCATATTTAGCTGTACCTGCTGCACCA
  AAAAAAGCTGTTGCACAACCAAAAACAGCTGTAAAAGCTTATGCTGTTACTAAACCTCAA
  ACGACTCAAACAGTTAGCAAAATTGCGCAAGTTAAACCAAACAACACTGGTATTCGTGCT
  TCTGTTTATGAAAAAACAGCGAAAAACGGCGCAAAATATGCGGATCGTACATTCTATGTA
  ACAAAAGAACGTGCACATGGTAATGAAACATATGTATTATTAAACAATACAAGTCATAAT
  ATTCCATTAGGTTGGTTCAATGTAAAAGACTTAAATGTTCAAAACTTAGGCAAAGAAGTT
  AAAACAACTCAAAAATATACTGTTAATAAATCAAATAACGGCTTATCACTGGTTCCTTGG
  GGTACTAAAAACCAAGTCATTTTAACAGGCAATAACATTGCTCAAGGTACATTTAATGCA
  ACGAAACAAGTATCTGTAGGCAAAGATGTTTATTTATACGGTACTATTAATAACCGCACT
  GGTTGGGTAAATGCAAAAGATTTAACTGCACCAACTGCTGTTAAACCAACTACATCAGCT
  GCCAAAGATTATAACTACACTTATGTAATTAAAAATGGTAATGGTTATTACTATGTAACA
  CCAAATTCTGATACAGCTAAATACTCATTAAAAGCATTTAATGAACAACCATTCGCAGTT
  GTTAAAGAACAAGTCATTAATGGACAAACTTGGTACTATGGTAAATTATCTAACGGTAAA
  TTAGCATGGATTAAATCAACTGATTTAGCTAAAGAATTAATTAAGTATAATCAAACAGGT
  ATGACATTAAACCAAGTTGCTCAAATACAAGCTGGTTTACAATATAAACCACAAGTACAA
  CGTGTACCAGGTAAGTGGACAGATGCTAACTTTAATGATGTTAAGCATGCAATGGATACG
  AAGCGTTTAGCTCAAGATCCAGCATTAAAATATCAATTCTTACGCTTAGACCAACCACAA
  AATATTTCTATTGATAAAATTAATCAATTCTTAAAAGGTAAAGGTGTATTAGAAAACCAA
  GGTGCTGCATTTAACAAAGCTGCTCAAATGTATGGCATTAATGAAGTTTATCTTATCTCA
  CATGCCCTATTAGAAACAGGTAACGGTACTTCTCAATTAGCAAAAGGTGCAGATGTAGTG
  AACAACAAAGTTGTAACTAACTCTAACACGAAATACCATAACGTATTTGGTATTGCTGCA
  TATGATAACGATCCTTTACGTGAAGGTATTAAATATGCTAAACAAGCTGGTTGGGACACA
  GTATCAAAAGCAATCGTTGGTGGTGCTAAATTCATCGGCAACTCATATGTAAAAGCTGGT
  CAAAATACGCTTTACAAAATGAGATGGAATCCTGCACATCCAGGAACACACCAATATGCA
  ACAGATGTAGATTGGGCTAACATCAATGCTAAAATCATCAAAGGCTACTATGATAAAATT
  GGCGAAGTCGGCAAATACTTCGACATCCCACAATATAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1980
  2040
  2100
  2160
  2220
  2280
  2340
  2400
  2460
  2520
  2580
  2640
  2700
  2760
  2820
  2880
  2940
  3000
  3060
  3120
  3180
  3240
  3300
  3360
  3420
  3480
  3540
  3600
  3660
  3720
  3762
  

⊟Protein

⊟General

• locus tag: JSNZ_001017
• symbol: Atl
• description: bifunctional autolysin
• length: 1253
• theoretical pI: 10.0745
• theoretical MW: 137048
• GRAVY: -0.614844

⊟Function

• reaction:
  EC 3.2.1.96?  ExPASy
  Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)₂)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact
  EC 3.5.1.28?  ExPASy
  N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds V-type ATPase, D subunit (TIGR00309; EC 3.6.3.14; HMM-score: 10)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 55.1)
  SH3 (CL0010) GW; GW (Gly-Tryp) dipeptide domain (PF13457; HMM-score: 53.6)
  no clan defined Amidase_2; N-acetylmuramoyl-L-alanine amidase (PF01510; HMM-score: 49.4)
  and 6 more
  DGOK; 2-keto-3-deoxy-galactonokinase (PF05035; HMM-score: 23.1)
  SH3 (CL0010) SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 17.6)
  E-set (CL0159) Fn3-III_PB4; Straight fiber protein PB4 third Fn3-like domain (PF24171; HMM-score: 13.4)
  no clan defined AvrPiz-t; Avirulence protein AvrPiz-t (PF22347; HMM-score: 13.3)
  DUF780; Caenorhabditis elegans protein of unknown function (DUF780) (PF05611; HMM-score: 12.8)
  LysM (CL0187) LysM2_NFP; NFP LysM2 domain (PF23457; HMM-score: 8.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.02
  • Extracellular Score: 9.98
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.0004
  • Cell wall & surface Score: 0.1082
  • Extracellular Score: 0.8914
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 29 aa
  • SP(Sec/SPI): 0.997209
  • TAT(Tat/SPI): 0.001289
  • LIPO(Sec/SPII): 0.001006
  • Cleavage Site: CS pos: 29-30. VQA-AE. Pr: 0.9829
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLDSNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVNGDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKSAAKPAALETQYKAAAPKAATTTAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPSTPSTGKLTVAANNGVAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLYGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYAVTKPQTTQTVSKIAQVKPNNTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVKTTQKYTVNKSNNGLSLVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications