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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0922 [new locus tag: NWMN_RS05165 ]
  • pan locus tag?: SAUPAN003260000
  • symbol: atl
  • pan gene symbol?: atl
  • synonym: atlA
  • product: bifunctional autolysin precursor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0922 [new locus tag: NWMN_RS05165 ]
  • symbol: atl
  • product: bifunctional autolysin precursor
  • replicon: chromosome
  • strand: -
  • coordinates: 1020866..1024636
  • length: 3771
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAAAAAATTCAATTACAAACTACCATCAATGGTTGCATTAACGCTTGTAGGTTCA
    GCAGTCACTGCACATCAAGTTCAAGCAGCTGAGACGACACAAGATCAAACTACTAATAAA
    AACGTTTTAGATAATAATAAAGTTAAAGCAACTACTGAACAAGCAAAAGCTGAGGTAAAA
    AATCCAACGCAAAACATTTCTGGCACTCAAGTATATCAAGACCCTGCTATTGTCCAACCA
    AAAACAGCAAATAACAAAACAGGCAATGCTCAAGTAAGTCAAAAAGTTGATACTGCACAA
    GTAAATGGTGACACTCGTGCTAATCAATCAGCGACTACAAATAATACGCAGCCTGTTGCA
    AAGTCAACAAGCACTACAGCACCTAAAACTAACACTAATGTTACAAATGCTGGTTATAGT
    TTAGTTGATGATGAAGATGATAATTCAGAAAATCAAATTAATCCAGAATTAATTAAATCA
    GCTGCTAAACCTGCAGCTCTTGAAACGCAATATAAAACCGCAGCACCTAAAGCTGCAACT
    ACATCAGCACCTAAAGCTAAAACTGAAGCGACACCTAAAGTAACTACTTTTAGCGCTTCA
    GCACAACCAAGATCAGTTGCTGCAACACCAAAAACGAGTTTGCCAAAATATAAACCACAA
    GTAAACTCTTCAATTAACGATTACATTCGTAAAAATAACTTAAAAGCACCTAAAATTGAA
    GAAGATTATACATCTTACTTCCCTAAATACGCATACCGTAACGGCGTAGGTCGTCCTGAA
    GGTATCGTAGTTCATGATACAGCTAATGATCGTTCGACGATAAATGGTGAAATTAGTTAT
    ATGAAAAATAACTATCAAAACGCATTCGTACATGCATTTGTTGATGGGGATCGTATAATC
    GAAACAGCACCAACGGATTACTTATCTTGGGGTGTCGGTGCAGTCGGTAACCCTAGATTC
    ATCAATGTTGAAATCGTACACACACACGACTATGCTTCATTTGCACGTTCAATGAATAAC
    TATGCTGACTATGCAGCTACACAATTACAATATTATGGTTTAAAACCAGACAGTGCTGAG
    TATGATGGAAATGGTACAGTATGGACTCACTACGCTGTAAGTAAATATTTAGGTGGTACT
    GACCATGCCGATCCACATGGATATTTAAGAAGTCATAATTATAGTTATGATCAATTATAT
    GACTTAATTAATGAAAAATATTTAATAAAAATGGGTAAAGTGGCGCCATGGGGTACGCAA
    TCTACAACTACCCCTACTACACCATCAAAACCAACAACACCGTCGAAACCATCAACTGGT
    AAATTAACAGTTGCTGCAAACAATGGTGTCGCACAAATCAAACCAACAAATAGTGGTTTA
    TATACTACTGTATACGACAAAACTGGTAAAGCAACTAATGAAGTTCAAAAAACATTTGCT
    GTATCTAAAACAGCTACATTAGGTAATCAAAAATTCTATCTTGTTCAAGATTACAATTCT
    GGTAATAAATTTGGTTGGGTTAAAGAAGGCGATGTGGTTTACAACACAGCTAAATCACCT
    GTAAATGTAAATCAATCATATTCAATCAAACCTGGTACGAAACTTTATACAGTACCTTGG
    GGTACATCTAAACAAGTTGCTGGTAGTGTGTCTGGCTCTGGAAACCAAACATTTAAGGCT
    TCAAAGCAACAACAAATTGATAAATCAATTTATTTATATGGCTCTGTGAATGGTAAATCT
    GGTTGGGTAAGTAAAGCATATTTAGTTGATACTGCTAAACCTACGCCTACACCAACACCT
    AAGCCATCAACACCTACAACAAATAATAAATTAACAGTTTCATCATTAAACGGTGTTGCT
    CAAATTAATGCTAAAAACAATGGCTTATTCACTACAGTTTATGACAAAACTGGTAAGCCA
    ACGAAAGAAGTTCAAAAAACATTTGCTGTAACAAAAGAAGCAAGTTTAGGTGGAAACAAA
    TTCTACTTAGTTAAAGATTACAATAGTCCAACTTTAATTGGTTGGGTTAAACAAGGTGAC
    GTTATTTATAACAATGCAAAATCACCTGTAAATGTAATGCAAACATATACAGTAAAACCA
    GGCACTAAATTATATTCAGTACCTTGGGGCACTTATAAACAAGAAGCTGGTGCAGTTTCT
    GGTACAGGTAACCAAACTTTTAAAGCGACTAAGCAACAACAAATTGATAAATCTATCTAT
    TTATTTGGAACTGTAAATGGTAAATCTGGTTGGGTAAGTAAAGCATATTTAGCTGTACCT
    GCTGCACCTAAAAAAGCAGTAGCACAACCAAAAACAGCTGTAAAAGCTTATACTGTTACT
    AAACCACAAACGACTCAAACAGTTAGCAAGATTGCTCAAGTTAAACCAAACAACACTGGT
    ATTCGTGCTTCTGTTTATGAAAAAACAGCGAAAAACGGTGCGAAATATGCAGACCGTACG
    TTCTATGTAACAAAAGAGCGTGCTCATGGTAATGAAACGTATGTATTATTAAACAATACA
    AGCCATAACATCCCATTAGGTTGGTTCAATGTAAAAGACTTAAATGTTCAAAACTTAGGC
    AAAGAAGTTAAAACGACTCAAAAATATACTGTTAATAAATCAAATAACGGCTTATCAATG
    GTTCCTTGGGGTACTAAAAACCAAGTCATTTTAACAGGCAATAACATTGCTCAAGGTACA
    TTTAATGCAACGAAACAAGTATCTGTAGGCAAAGATGTTTATTTATACGGTACTATTAAT
    AACCGCACTGGTTGGGTAAATGCAAAAGATTTAACTGCACCAACTGCTGTGAAACCAACT
    ACATCAGCTGCCAAAGATTATAACTACACTTATGTAATTAAAAATGGTAATGGTTATTAC
    TATGTAACACCAAATTCTGATACAGCTAAATACTCATTAAAAGCATTTAATGAACAACCA
    TTCGCAGTTGTTAAAGAACAAGTCATTAATGGACAAACTTGGTACTATGGTAAATTATCT
    AACGGTAAATTAGCATGGATTAAATCAACTGATTTAGCTAAAGAATTAATTAAGTATAAT
    CAAACAGGTATGACATTAAACCAAGTTGCTCAAATACAAGCTGGTTTACAATATAAACCA
    CAAGTACAACGTGTACCAGGTAAGTGGACAGGTGCTAACTTTAATGATGTTAAGCATGCA
    ATGGATACGAAGCGTTTAGCTCAAGATCCAGCATTAAAATATCAATTCTTACGCTTAGAC
    CAACCACAAAATATTTCTATTGATAAAATTAATCAATTCTTAAAAGGTAAAGGTGTATTA
    GAAAACCAAGGTGCTGCATTTAACAAAGCTGCTCAAATGTATGGCATTAATGAAGTTTAT
    CTTATCTCACATGCCCTATTAGAAACAGGTAACGGTACTTCTCAATTAGCGAAAGGTGCA
    GATGTAGTGAACAACAAAGTTGTAACTAACTCAAACACGAAATACCATAACGTATTTGGT
    ATTGCTGCATATGATAACGATCCTTTACGTGAAGGTATTAAATATGCTAAACAAGCTGGT
    TGGGACACAGTATCAAAAGCAATCGTTGGTGGTGCTAAATTCATCGGCAACTCATATGTA
    AAAGCTGGTCAAAATACACTTTACAAAATGAGATGGAATCCTGCACATCCAGGAACACAC
    CAATATGCTACAGATGTAGATTGGGCTAACATCAATGCTAAAATCATCAAAGGCTACTAT
    GATAAAATTGGCGAAGTCGGCAAATACTTCGACATCCCACAATATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
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    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3771

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0922 [new locus tag: NWMN_RS05165 ]
  • symbol: Atl
  • description: bifunctional autolysin precursor
  • length: 1256
  • theoretical pI: 10.1019
  • theoretical MW: 137391
  • GRAVY: -0.620303

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds V-type ATPase, D subunit (TIGR00309; EC 3.6.3.14; HMM-score: 9.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 54.1)
    SH3 (CL0010) SH3_8; SH3-like domain (PF13457; HMM-score: 46.8)
    and 3 more
    no clan defined DGOK; 2-keto-3-deoxy-galactonokinase (PF05035; HMM-score: 25.1)
    DUF780; Caenorhabditis elegans protein of unknown function (DUF780) (PF05611; HMM-score: 13.2)
    SH3 (CL0010) SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 10.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: HQVQAAET
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.997278
    • TAT(Tat/SPI): 0.001261
    • LIPO(Sec/SPII): 0.00098
    • Cleavage Site: CS pos: 29-30. VQA-AE. Pr: 0.9831
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLDNNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVNGDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKSAAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGVAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQYKPQVQRVPGKWTGANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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