PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000440
• pan locus tag^?: SAUPAN002240000
• symbol: JSNZ_000440
• pan gene symbol^?: prs
• synonym:
• product: ribose-phosphate diphosphokinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000440
• symbol: JSNZ_000440
• product: ribose-phosphate diphosphokinase
• replicon: chromosome
• strand: +
• coordinates: 470536..471501
• length: 966
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGTTAAATAATGAATATAAGAATTCGTCATTAAAGATTTTTTCATTGAAAGGAAACGAA
  GCATTAGCGCAAGAAGTTGCTGACCAAGTAGGAATTGAACTAGGTAAATGTTCAGTTAAA
  CGTTTTAGTGATGGAGAAATTCAAATTAATATCGAAGAGAGTATTCGTGGTTGTGACGTA
  TTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATG
  ATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGA
  TATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCA
  AACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAA
  ATTCAAGGATTCTTTGATATTCCAATTGACCACTTAATGGGTGTGCCAATTCTTGCTAAA
  CATTTCAAAGATGATCCGAATATTAACCCAGAAGAATGTGTCGTTGTTTCACCAGACCAT
  GGCGGCGTTACACGTGCACGTAAATTAGCTGACATTTTAAAAACTCCAATTGCAATTATA
  GATAAACGTCGTCCTAGACCAAATGTTGCTGAAGTGATGAACATTGTTGGTGAGATTGAA
  GGACGTACGGCAATTATTATTGACGATATTATTGATACAGCAGGTACAATCACTTTAGCT
  GCACAAGCATTAAAAGATAAAGGTGCTAAAGAAGTATATGCTTGTTGTACACACCCTGTT
  TTATCAGGACCGGCTAAAGAACGTATCGAAAATTCTGCTATAAAAGAATTAATCGTAACA
  AACTCAATTCATTTAGATGAAGATCGCAAACCATCTAACACTAAAGAATTATCTGTTGCT
  GGTTTAATCGCACAAGCTATCATTCGTGTATACGAAAGAGAATCAGTTAGCGTATTATTT
  GACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  966
  

⊟Protein

⊟General

• locus tag: JSNZ_000440
• symbol: JSNZ_000440
• description: ribose-phosphate diphosphokinase
• length: 321
• theoretical pI: 6.17934
• theoretical MW: 35283.6
• GRAVY: -0.0314642

⊟Function

• reaction:
  EC 2.7.6.1?  ExPASy
  Ribose-phosphate diphosphokinase ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 420.2)
  and 7 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 26.7)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 21.8)
  Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 20.1)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR00336; EC 2.4.2.10; HMM-score: 17.6)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 15.6)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis amidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 13.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uracil phosphoribosyltransferase (TIGR01091; EC 2.4.2.9; HMM-score: 12.9)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  PRTase-like (CL0533) Pribosyltran_N; N-terminal domain of ribose phosphate pyrophosphokinase (PF13793; HMM-score: 185)
  and 4 more
  Pribosyl_synth; Phosphoribosyl synthetase-associated domain (PF14572; HMM-score: 98.7)
  Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 74.1)
  UPRTase; Uracil phosphoribosyltransferase (PF14681; HMM-score: 14.1)
  PRTase-CE; PRTase-CE (PF24390; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7455
  • Cytoplasmic Membrane Score: 0.0039
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.2502
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.013988
  • TAT(Tat/SPI): 0.000517
  • LIPO(Sec/SPII): 0.00133
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSDGEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYARQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAKHFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGEIEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKELIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications