PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000023
• pan locus tag^?: SAUPAN000035000
• symbol: JSNZ_000023
• pan gene symbol^?: walI
• synonym:
• product: two-component system regulatory protein YycI

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000023
• symbol: JSNZ_000023
• product: two-component system regulatory protein YycI
• replicon: chromosome
• strand: +
• coordinates: 28271..29059
• length: 789
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  
  ATGAACTGGAAACTGACGAAGACACTTTTCATTTTCGTGTTTATTCTTGTCAACATCGTG
  TTAGTATCGATTTATGTTAATAAAGTCAATCGCTCACACATTAATGAGGTTGAGAGTAAC
  AATGAAGTTAATTTTCAGCAAGAAGAAATTAAAGTACCGGCTAGTATTTTGAATAAATCA
  GTTAAAGGTATAAAATTAGAGCAAATTACAGGGCGATCAAAAGACTTTAGTTCTAAAGCT
  AAAGGCGATTCGGATTTGACCACATCAGATGGTGGAAAATTATTAAATGCGAACATTAGT
  CAATCGGTAAAGGTCAGTGACAATAACTTAAAAGATTTGAAAGATTATGTTAACAAACGT
  GTGTTCAAAGGTTCAGAATATCAATTAAGTGAAATTAGTTCTGGTTCTGTAAAATACGAA
  CAAACGTATGATAATTTCCCGATTTTGAACAATAGTAAAGCGATGTTGAACTTTAATATA
  GAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGAT
  GGTGCAGATAAGAAGCATCAAGTTATTAGTGTGAGAAAAGCAATCGAGGCATTATATTAT
  AATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTTGGTTACTATTCAGTC
  GTGAATGAAACGAATGTTCAATTGTTACAACCAAACTGGGAAATTAAAGTGAAGCATGAC
  GGTAAGGACAAAACGAATACTTACTATGTCGAAGCGACAAATAATAACCCTAAAATTATT
  AATCATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  789
  

⊟Protein

⊟General

• locus tag: JSNZ_000023
• symbol: JSNZ_000023
• description: two-component system regulatory protein YycI
• length: 262
• theoretical pI: 9.61263
• theoretical MW: 29888.6
• GRAVY: -0.611832

⊟Function

• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions cytidylate kinase (TIGR00017; EC 2.7.4.14; HMM-score: 11.7)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  YycI_YycH (CL0285) YycI; YycI protein (PF09648; HMM-score: 192.6)
  and 2 more
  GAF (CL0161) HrcA; HrcA protein C terminal domain (PF01628; HMM-score: 12.8)
  no clan defined DUF5889; Family of unknown function (DUF5889) (PF19237; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9988
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0012
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.432663
  • TAT(Tat/SPI): 0.000869
  • LIPO(Sec/SPII): 0.040979
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVNFQQEEIKVPASILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQSVKVSDNNLKDLKDYVNKRVFKGSEYQLSEISSGSVKYEQTYDNFPILNNSKAMLNFNIEDNKATSYKQSMMDDIKPTDGADKKHQVISVRKAIEALYYNRYLKKGDEVINARLGYYSVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Olivier Poupel, Mati Moyat, Julie Groizeleau, Luísa C S Antunes, Simonetta Gribaldo, Tarek Msadek, Sarah Dubrac
Transcriptional Analysis and Subcellular Protein Localization Reveal Specific Features of the Essential WalKR System in Staphylococcus aureus.
PLoS One: 2016, 11(3);e0151449
[PubMed:26999783] [WorldCat.org] [DOI] (I e)