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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04960 [old locus tag: SACOL0968 ]
  • pan locus tag?: SAUPAN003073000
  • symbol: SACOL_RS04960
  • pan gene symbol?: spsA
  • synonym:
  • product: inactive signal peptidase IA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS04960 [old locus tag: SACOL0968 ]
  • symbol: SACOL_RS04960
  • product: inactive signal peptidase IA
  • replicon: chromosome
  • strand: +
  • coordinates: 970707..971231
  • length: 525
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (970707..971231) NCBI
  • BioCyc: SACOL_RS04960 BioCyc
  • MicrobesOnline: see SACOL0968

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    GTGAAAAAAGTTGTAAAATATTTGATTTCATTGATACTTGCTATTATCATTGTACTGTTC
    GTACAAACTTTTGTAATAGTTGGTCATGTCATTCCGAATAATGATATGTCACCAACCCTT
    AACAAAGGGGATCGTGTTATTGTAAATAAAATTAAAGTTACATTTAATCAATTGAATAAT
    GGTGATATCATTACATATAGGCGTGGTAACGAGATATATACTAGTCGAATTATTGCCAAA
    CCTGGTCAATCAATGGCGTTTCGTCAGGGACAATTATACCGTGATGACCGACCGGTTGAC
    GCATCTTATGCCAAGAACAGAAAAATTAAAGATTTTAGTTTGCGCAATTTTAAAGAATTA
    GATGGTGATATTATTCCGCCAAACAATTTTGTTGTGCTAAATGATCAAGATAATAACAAG
    CACGATTCAAGACAATTTGGTTTAATCGATAAAAAGGATATTATTGGTAATGTTAGTTTA
    CGATACTATCCTTTTTCAAAATGGACTGTTCAGTTCAAATCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    525

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS04960 [old locus tag: SACOL0968 ]
  • symbol: SACOL_RS04960
  • description: inactive signal peptidase IA
  • length: 174
  • theoretical pI: 10.4619
  • theoretical MW: 20109.1
  • GRAVY: -0.310345

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal peptidase I (TIGR02227; EC 3.4.21.89; HMM-score: 152.6)
    and 4 more
    Cellular processes Cellular processes Detoxification nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 40.4)
    Genetic information processing Protein fate Protein modification and repair nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 40.4)
    conjugative transfer signal peptidase TraF (TIGR02771; HMM-score: 32)
    signal peptidase I (TIGR02228; EC 3.4.21.89; HMM-score: 28.7)
  • TheSEED: see SACOL0968
  • ⊞PFAM:
    Peptidase_SF (CL0299) Peptidase_S26; Signal peptidase, peptidase S26 (PF10502; HMM-score: 113.1)
    and 5 more
    Peptidase_S24; Peptidase S24-like (PF00717; HMM-score: 19.5)
    no clan defined DUF2534; Protein of unknown function (DUF2534) (PF10749; HMM-score: 15.8)
    Neurensin; Neurensin/Transmembrane protein 74 (PF14927; HMM-score: 12.3)
    RBG (CL0760) AsmA; AsmA family (PF05170; HMM-score: 12.1)
    no clan defined E1-E2_ATPase; E1-E2 ATPase (PF00122; HMM-score: 9.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.53
    • Cellwall Score: 8.76
    • Extracellular Score: 0.7
    • Internal Helix: 1
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0006
    • Cytoplasmic Membrane Score: 0.9907
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0084
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.473598
    • TAT(Tat/SPI): 0.000476
    • LIPO(Sec/SPII): 0.051893
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI: 446680869 NCBI
  • RefSeq: WP_000758215 NCBI
  • UniProt: see SACOL0968

⊟Protein sequence[edit | edit source]

  • MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGDRVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDASYAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSLRYYPFSKWTVQFKS

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0968
  • protein localization: see SACOL0968
  • quantitative data / protein copy number per cell:
  • ⊟interaction partners:
    SACOL_RS00470peptidoglycan-binding protein LysM  [1] (data from MRSA252)
    SACOL_RS01040formate acetyltransferase  [1] (data from MRSA252)
    SACOL_RS01135L-lactate dehydrogenase  [1] (data from MRSA252)
    SACOL_RS02190GTP-binding protein YchF  [1] (data from MRSA252)
    SACOL_RS0220030S ribosomal protein S6  [1] (data from MRSA252)
    SACOL_RS02305hypothetical protein  [1] (data from MRSA252)
    SACOL_RS02330IMP dehydrogenase  [1] (data from MRSA252)
    SACOL_RS02640YbaB/EbfC family nucleoid-associated protein  [1] (data from MRSA252)
    SACOL_RS0303050S ribosomal protein L1  [1] (data from MRSA252)
    SACOL_RS0303550S ribosomal protein L10  [1] (data from MRSA252)
    SACOL_RS0304050S ribosomal protein L7/L12  [1] (data from MRSA252)
    SACOL_RS03050DNA-directed RNA polymerase subunit beta  [1] (data from MRSA252)
    SACOL_RS03055DNA-directed RNA polymerase subunit beta'  [1] (data from MRSA252)
    SACOL_RS0307030S ribosomal protein S7  [1] (data from MRSA252)
    SACOL_RS043252,3-bisphosphoglycerate-independent phosphoglycerate mutase  [1] (data from MRSA252)
    SACOL_RS04330enolase  [1] (data from MRSA252)
    SACOL_RS04840NADH dehydrogenase  [1] (data from MRSA252)
    SACOL_RS05470bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase  [1] (data from MRSA252)
    SACOL_RS05630pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SACOL_RS05635pyruvate dehydrogenase E1 component subunit beta  [1] (data from MRSA252)
    SACOL_RS05640dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex  [1] (data from MRSA252)
    SACOL_RS05645dihydrolipoyl dehydrogenase  [1] (data from MRSA252)
    SACOL_RS06140cell division protein FtsZ  [1] (data from MRSA252)
    SACOL_RS0642050S ribosomal protein L19  [1] (data from MRSA252)
    SACOL_RS0650530S ribosomal protein S2  [1] (data from MRSA252)
    SACOL_RS06515elongation factor Ts  [1] (data from MRSA252)
    SACOL_RS06770glutamine synthetase  [1] (data from MRSA252)
    SACOL_RS07385dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    SACOL_RS0868050S ribosomal protein L21  [1] (data from MRSA252)
    SACOL_RS08795trigger factor  [1] (data from MRSA252)
    SACOL_RS0882050S ribosomal protein L20  [1] (data from MRSA252)
    SACOL_RS08905isocitrate dehydrogenase (NADP(+))  [1] (data from MRSA252)
    SACOL_RS08930pyruvate kinase  [1] (data from MRSA252)
    SACOL_RS0904530S ribosomal protein S4  [1] (data from MRSA252)
    SACOL_RS10210non-heme ferritin  [1] (data from MRSA252)
    SACOL_RS11070UDP-N-acetylglucosamine 1-carboxyvinyltransferase  [1] (data from MRSA252)
    SACOL_RS11435Asp23/Gls24 family envelope stress response protein  [1] (data from MRSA252)
    SACOL_RS11650DNA-directed RNA polymerase subunit alpha  [1] (data from MRSA252)
    SACOL_RS1165530S ribosomal protein S11  [1] (data from MRSA252)
    SACOL_RS1168550S ribosomal protein L15  [1] (data from MRSA252)
    SACOL_RS1172550S ribosomal protein L24  [1] (data from MRSA252)
    SACOL_RS1175030S ribosomal protein S3  [1] (data from MRSA252)
    SACOL_RS1176550S ribosomal protein L2  [1] (data from MRSA252)
    SACOL_RS1177550S ribosomal protein L4  [1] (data from MRSA252)
    SACOL_RS13365pyruvate oxidase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jump up to: 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS04960&oldid=62910"
  • This page was last edited on 10 March 2016, at 16:42.
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