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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1967 [new locus tag: SACOL_RS10285 ]
  • pan locus tag?: SAUPAN004935000
  • symbol: SACOL1967
  • pan gene symbol?: pcrB
  • synonym:
  • product: geranylgeranylglyceryl phosphate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1967 [new locus tag: SACOL_RS10285 ]
  • symbol: SACOL1967
  • product: geranylgeranylglyceryl phosphate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 2030888..2031580
  • length: 693
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTATGACATTAAAAAATGGCGCCATATTTTTAAATTAGACCCAGCTAAACATATTTCA
    GATGATGATTTAGATGCGATTTGTATGTCTCAAACAGATGCAATTATGATTGGTGGAACT
    GATGACGTTACTGAAGATAATGTCATTCATTTAATGAGCAGAGTAAGAAGATACCCATTG
    CCTTTAGTACTTGAAATATCAAACATCGAAAGTGTAATGCCTGGTTTTGATTTTTATTTT
    GTACCTACAGTACTGAACAGTACAGATGTTGTATTTCACAATGGTACATTATTAGAAGCG
    CTTAAAACATATGGACATAGTATAGATTTTGAGGAAGTAATATTTGAAGGGTATGTCGTG
    TGCAATGCTGATAGCAAAGTGGCAAAACATACCAAAGCAAATACAGATTTAACAACAGAA
    GATTTAGAAGCATATGCCCAAATGGTCAATCATATGTATCGATTACCGGTTATGTATATA
    GAGTATAGTGGCATTTATGGCGACGTATCAAAGGTTCAAGCTGTCTCAGAACATCTAACA
    GAAACGCAACTTTTTTATGGTGGCGGTATTTCCTCAGAACAACAAGCGACAGAGATGGCA
    GCTATTGCAGATACAATTATCGTCGGTGATATTATTTATAAAGATATTAAAAAAGCTTTA
    AAAACAGTAAAAATAAAGGAGTCTAGTAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    693

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1967 [new locus tag: SACOL_RS10285 ]
  • symbol: SACOL1967
  • description: geranylgeranylglyceryl phosphate synthase
  • length: 230
  • theoretical pI: 4.67385
  • theoretical MW: 25888.4
  • GRAVY: -0.0947826

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.5.1.n9?  ExPASy
    Heptaprenylglyceryl phosphate synthase All-trans-heptaprenyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(all-trans-heptaprenyl)glycerol 1-phosphate
  • ⊞TIGRFAM:
    phosphoglycerol geranylgeranyltransferase (TIGR04146; EC 2.5.1.41; HMM-score: 245.3)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 204.4)
    and 3 more
    phosphoglycerol geranylgeranyltransferase, putative (TIGR04147; EC 2.5.1.41; HMM-score: 187.6)
    phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 46.9)
    HAD superfamily (subfamily IG) hydrolase, 5'-nucleotidase (TIGR02244; HMM-score: 17.3)
  • TheSEED  :
    • Geranylgeranylglyceryl phosphate synthase-like protein YerE
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  (S)-3-O-geranylgeranylglyceryl phosphate synthase
  • ⊞PFAM:
    TIM_barrel (CL0036) PcrB; PcrB family (PF01884; HMM-score: 234.6)
    and 4 more
    Trp_syntA; Tryptophan synthase alpha chain (PF00290; HMM-score: 21.4)
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 13.3)
    Tubulin_C (CL0442) Tubulin_C; Tubulin C-terminal domain (PF03953; HMM-score: 12.6)
    no clan defined Pox_H7; Late protein H7 (PF04787; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9444
    • Cytoplasmic Membrane Score: 0.0428
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0127
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002072
    • TAT(Tat/SPI): 0.000145
    • LIPO(Sec/SPII): 0.000433
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVTEDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALKTYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYIEYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKKALKTVKIKESSK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell: 169 [3]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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