PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL2517 [new locus tag: SACOL_RS13180 ]
pan locus tag^?: SAUPAN006133000
symbol: SACOL2517
pan gene symbol^?: —
• synonym:
• product: MerR family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL2517 [new locus tag: SACOL_RS13180 ]
• symbol: SACOL2517
• product: MerR family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 2576850..2577614
• length: 765
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3238292 NCBI
• RefSeq: YP_187311 NCBI
• BioCyc: see SACOL_RS13180
• MicrobesOnline: 913989 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCTAACTATTCGACTGGAGAACTCGCAAAATTATGCAATGTGACAACACGAACGATT
  CAATATTATGATCGCAAAGGTATTTTGAAACCACAAGGATTTACAGAAGGAAAGCGTCGT
  GTGTATACAGAACAACAGCGACAAACATTAGAGTTAATCTTATTGCTTAAAGATTTAGGT
  TGTGCGTTAAGCGATATAGATATGTTGCTAAAAGGTGAAGGTACTTTGAAGACGCTCAAT
  ACTTTACTAACTATGAAACAACAAGAAATTAACCAACAAGTTAAACAGCAACAAGCGGTA
  TTAAACAAAATTAAAAATGTTCAATATTACGTAAATGAAGCGTCGACGTCTCCAATCACA
  CACTTAAAAGACATAGAGCATGTCATGAGTAAATCAGCTGAAATGAAAAGTATTCGTCGT
  AACATTTGGATTAGTGCTGGTATTATAGGAATAATTCAATATTCTAGCATTATAAGTGCA
  ATTTTGATGAAAAATAAATGGCCGTTTTTAATTGCTTTACCATTTATGATTGGTTATGGC
  ATTGGTGTTACTTTTTATTACCAACAAAAGGTTGCCTATTTATGTCCTAACTGCCAGCAT
  ATATTCTCACCATCTTTGTGGGCAGTTATCAAAGCGAAACATACAGCGACAACACGTCGA
  TTCGAATGTCCAAACTGTCATGAAACGCATTATTGCATTGAAGTACCTAAAGCGCATATG
  AGTACAGAACAATTAGAAATATCCCACATACAACATAACAATTAA
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⊟Protein

⊟General

• locus tag: SACOL2517 [new locus tag: SACOL_RS13180 ]
• symbol: SACOL2517
• description: MerR family transcriptional regulator
• length: 254
• theoretical pI: 9.42155
• theoretical MW: 29141.8
• GRAVY: -0.238189

⊟Function

• ⊞TIGRFAM:
  Signal transduction Regulatory functions DNA interactions Zn(II)-responsive transcriptional regulator (TIGR02043; HMM-score: 59)
  and 8 more
  Cellular processes Cellular processes Detoxification Hg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 44.9)
  Signal transduction Regulatory functions DNA interactions Hg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 44.9)
  Signal transduction Regulatory functions DNA interactions Cd(II)/Pb(II)-responsive transcriptional regulator (TIGR02047; HMM-score: 44.5)
  Signal transduction Regulatory functions DNA interactions Cu(I)-responsive transcriptional regulator (TIGR02044; HMM-score: 40.5)
  Cellular processes Cellular processes Detoxification redox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 19.6)
  Signal transduction Regulatory functions DNA interactions redox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 19.6)
  cxxc_20_cxxc protein (TIGR04104; HMM-score: 16.1)
  MJ0042 family finger-like domain (TIGR02098; HMM-score: 5.7)
• TheSEED  :
  • Transcriptional regulator, MerR family
  Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Cobalt-zinc-cadmium resistance  Transcriptional regulator, MerR family
• ⊞PFAM:
  HTH (CL0123) MerR_1; MerR HTH family regulatory protein (PF13411; HMM-score: 61.7)
  and 25 more
  MerR; MerR family regulatory protein (PF00376; HMM-score: 46.7)
  HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 19.6)
  MerR-DNA-bind; MerR, DNA binding (PF09278; HMM-score: 18.8)
  RING (CL0229) C1_4; TFIIH C1-like domain (PF07975; HMM-score: 17)
  Zn_Beta_Ribbon (CL0167) HVO_2753_ZBP; Small zinc finger protein HVO_2753-like, Zn-binding pocket (PF07754; HMM-score: 16.8)
  Zn_ribbon_4; zinc-ribbon domain (PF13717; HMM-score: 15.4)
  Tbcl_zf (CL0839) DUF4379; Probable Treble clef zinc finger domain (PF14311; HMM-score: 14.6)
  no clan defined RAC_head; Ribosome-associated complex head domain (PF16717; HMM-score: 14.5)
  Zn_Beta_Ribbon (CL0167) Zn_ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 14)
  Zn_ribbon_20; Zinc beta-ribbon domain (PF23551; HMM-score: 13.8)
  C2H2-zf (CL0361) zf-Di19; Drought induced 19 protein (Di19), zinc-binding (PF05605; HMM-score: 12.1)
  Zn_Beta_Ribbon (CL0167) Cas12f1-like_TNB; Cas12f1-like, TNB domain (PF07282; HMM-score: 11.8)
  Zn_ribbon_16; Zinc beta-ribbon finger, putative (PF21957; HMM-score: 11.8)
  Zn_ribbon_10; ER junction formation factor lunapark, zinc ribbon finger (PF10058; HMM-score: 10.9)
  no clan defined Opy2; Opy2 protein (PF09463; HMM-score: 10.8)
  Zn_Beta_Ribbon (CL0167) Zn_ribbon_FGT1_1; FORGETTER1 first zinc ribbon domain (PF23547; HMM-score: 10.6)
  Zn_ribbon_Nudix; Nudix N-terminal (PF14803; HMM-score: 10.3)
  Zn_ribbon_TFIIS; Transcription factor S-II (TFIIS) (PF01096; HMM-score: 10.2)
  no clan defined SlyX; SlyX (PF04102; HMM-score: 10.1)
  Tbcl_zf (CL0839) zf_Rg; Reverse gyrase zinc finger (PF17915; HMM-score: 10.1)
  no clan defined DUF7385; Family of unknown function (DUF7385) (PF24110; HMM-score: 8.9)
  C2H2-zf (CL0361) zf-H2C2_2; Zinc-finger double domain (PF13465; HMM-score: 8.5)
  Zn_Beta_Ribbon (CL0167) DUF7560; Family of unknown function (DUF7560) (PF24441; HMM-score: 8.1)
  CpXC; CpXC protein (PF14353; HMM-score: 7)
  no clan defined YnfU; YnfU (PF23499; HMM-score: 6.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 2
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0141
  • Cytoplasmic Membrane Score: 0.9533
  • Cell wall & surface Score: 0.0041
  • Extracellular Score: 0.0284
• ⊞LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004422
  • TAT(Tat/SPI): 0.000282
  • LIPO(Sec/SPII): 0.000685
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 57651015 NCBI
• RefSeq: YP_187311 NCBI
• UniProt: A0A0H2WX82 UniProt

⊟Protein sequence

• MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTEQQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLNKIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISAILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATTRRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: Integral membrane
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: gntP < gntK < gntR < SACOL2517

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Benjamin P Howden, Danielle J Smith, Ashley Mansell, Paul D R Johnson, Peter B Ward, Timothy P Stinear, John K Davies
Different bacterial gene expression patterns and attenuated host immune responses are associated with the evolution of low-level vancomycin resistance during persistent methicillin-resistant Staphylococcus aureus bacteraemia.
BMC Microbiol: 2008, 8;39
[PubMed:18304359] [WorldCat.org] [DOI] (I e)