From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2515 [new locus tag: SACOL_RS13170 ]
  • pan locus tag?: SAUPAN006131000
  • symbol: gntK
  • pan gene symbol?: gntK
  • synonym:
  • product: gluconokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2515 [new locus tag: SACOL_RS13170 ]
  • symbol: gntK
  • product: gluconokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2574439..2575992
  • length: 1554
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAATATATGATTGGTGTCGACATTGGAACGACGAGTACCAAGTCAGTCTTATATGAT
    GAAAATGGAACTTTTATCATGAAACATCAAATAGGCTATGATTTACACACACCAAACGTT
    GATGTCTCAGAAGAAAACCCAGATGAATTATTTGATGCAGTACTAATGACTATTAAATAT
    GTTATGAGAGAATCCAAAGTTAATAAAGACGACATTAAGTTTGTGTCATTTAGTGCGCAA
    ATGCATAGCTTGATTGCGATGGATCAGCAACATCAAAGATTAACAAATAATATTACTTGG
    GCAGATAACCGCGCTGCAAAATATGCAACAGTAATAAATGAAGTGCATGATGGCAATGCG
    ATTTACCAGCGAACAGGCACACCGATTCATCCTATGTCGCCATTAGCGAAGATTTTTTGG
    ATGAAACATGAATGGCAAGATGTATTTCAACGTACTGCTAAGTTTGCAGATATTAAAACA
    TACATTTTCTATCATTTATTTGATACATATATCATTGATTATTCAATGGCTTCCGCAACA
    GGGATGTTTAATTTAGAAACATTAGATTGGGATGTTGAGGCATTAGAACTGCTGGGTATT
    TCCAAGGAAATGTTGCCGGAATTAGTTCCAACAACGTATGTGATGAAAGGTATGAAAGAA
    CGTTATGCAACATTAATGGGGCTTAATAAAGATACACCGTTTGTTATTGGTGCGAGTGAT
    GGGGTCCTTTCTAATTTAGGTGTCAATAGTGTTGGTAAAGGAGAAGTTGCTGTCACAATC
    GGTACATCTGGGGCGATTCGTACTGTGATAGATAAACCGCGTACTGATTACAAAGGTAGA
    ATATTTTGTTATGTCTTAACAGAGGACCACTACGTCATCGGAGGTCCTGTAAATAATGGT
    GGTGTCGTATTGAGATGGTTGCGCGACGAGTTGCTAGCGAGTGAAGTCGAAACTGCGAAA
    CGTCTCGGTGTTGATCCTTATGATGTCTTAACTCAAATTGCAAAGCGTGTTAAACCAGGT
    GCAGATGGTTTAATATTCCATCCCTATTTAGCAGGAGAACGTGCGCCGCTTTGGAACGCA
    AATGCAAGAGGTTCATTCTTCGGTTTAACTTTATCTCATAAAAAAGAACATATGATTCGC
    GCTGCATTAGAAGGTGTTCTTTACAATTTGTATACCGTCTATCTTGCGCTTATTGAAGTA
    ATGAATGAAACACCTAAGATGATAAAAGCAACAGGTGGTTTTGCGAAAAGTGAAGTATGG
    CGTCAAATGATGTCAGATATATTTGACACAGAGTTAGTGGTTCCTGAAAGTTATGAAAGT
    TCATGCTTAGGTGCCTGCGTGCTCGGACTTAAAGCTGTAGGTGACATTGAAGATTTTTCA
    ATCGTTTCATCGATGGTCGGTGCCACAAACAATCATACGCCGATTGAAGAAAATGTCACT
    GTTTACCAAGAGATCGTATCCATTTTTATCAATTTAAGTCGTTCTTTAACAGAGAATTAT
    GAACAAATTGCAGATTTTCAACGCCAACATATCGCTGAAAATAAAACACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1554

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2515 [new locus tag: SACOL_RS13170 ]
  • symbol: GntK
  • description: gluconokinase
  • length: 517
  • theoretical pI: 5.23399
  • theoretical MW: 58094.1
  • GRAVY: -0.12089

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.1.12?  ExPASy
    Gluconokinase ATP + D-gluconate = ADP + 6-phospho-D-gluconate
  • ⊞TIGRFAM:
    Metabolism Energy metabolism Sugars gluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 1017.3)
    and 8 more
    Metabolism Energy metabolism Sugars xylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 338.7)
    Metabolism Energy metabolism Other glycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 211.4)
    Metabolism Energy metabolism Sugars L-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 117.4)
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 100.7)
    Metabolism Energy metabolism Sugars ribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 62.7)
    rhamnulokinase (TIGR02627; EC 2.7.1.5; HMM-score: 60.7)
    putative CoA-substrate-specific enzyme activase (TIGR00241; HMM-score: 21.2)
    benzoyl-CoA reductase, subunit A (TIGR02259; EC 1.3.7.8; HMM-score: 11.1)
  • TheSEED  :
    • Gluconokinase (EC 2.7.1.12)
    Carbohydrates Monosaccharides D-gluconate and ketogluconates metabolism  Gluconokinase (EC 2.7.1.12)
  • ⊞PFAM:
    Actin_ATPase (CL0108) FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 245.6)
    and 5 more
    FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 108.8)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 30.9)
    Hydant_A_N; Hydantoinase/oxoprolinase N-terminal region (PF05378; HMM-score: 14.5)
    no clan defined DDE_Tnp_IS66_C; IS66 C-terminal element (PF13817; HMM-score: 13.4)
    HTH (CL0123) PSV_trans_reg_dom; PSV, transcriptional regulator domain (PF22376; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9682
    • Cytoplasmic Membrane Score: 0.0212
    • Cell wall & surface Score: 0.003
    • Extracellular Score: 0.0075
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003874
    • TAT(Tat/SPI): 0.000098
    • LIPO(Sec/SPII): 0.000736
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSEENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWADNRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKTYIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGMKERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTDYKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIAKRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYTVYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGLKAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQRQHIAENKTQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulators: CcpA regulon, GntR (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL2515&oldid=92129"