From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2296 [new locus tag: SA_RS13165 ]
  • pan locus tag?: SAUPAN006133000
  • symbol: SA2296
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2296 [new locus tag: SA_RS13165 ]
  • symbol: SA2296
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2579359..2580123
  • length: 765
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTCTAACTATTCGACTGGAGAACTCGCAAAATTATGCAATGTGACAACACGAACGATT
    CAATATTATGATCGCAAAGGTATTTTGAAACCACAAGGATTTACAGAAGGAAAGCGTCGT
    GTGTATACAGAACAACAGCGACAAACATTAGAGTTAATCTTATTGCTTAAAGATTTAGGT
    TGTGCGTTAAGCGATATAGATATGTTGCTAAAAGGTGAAGGTACTTTGAAGACACTCAAT
    ACCTTACTAACCATGAAACAACAAGAAATTAACCAACAAGTCAAACAGCAACAAGCGGTA
    TTAAACAAAATTAAAAATGTTCAATATTACGTAAATGAAGCGTCGACGTCTCCAATCACA
    CACTTAAAAGACATAGAGCATGTCATGAGTAAATCAGCTGAAATGAAAAGTATTCGTCGT
    AACATTTGGATTAGTGCTGGTATTATAGGAATAATTCAATATTCTAGCATTATGAGTTCA
    ATCTTGATGAAAAACAAATGGCCGTTTTTAATTGCTTTACCATTTATGAATTGTTACGGC
    ATTGGTGTTACTTTTTATTACCAACAAAAGGTTGCCTATTTATGTCCTAACTGCCAGCAT
    ATATTCTCACCATCTTTGTGGGCAGTTATCAAAGCGAAACATACAGCGACAACACGTCGA
    TTCGAATGTCCGAACTGTCATGAAACGCATTATTGCATTGAAGTACCTAAAGCGCATATG
    AGTACAGAACAATTAGAAATATCCCACATACAACATAACAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    765

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2296 [new locus tag: SA_RS13165 ]
  • symbol: SA2296
  • description: hypothetical protein
  • length: 254
  • theoretical pI: 9.30204
  • theoretical MW: 29222.8
  • GRAVY: -0.27874

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions Zn(II)-responsive transcriptional regulator (TIGR02043; HMM-score: 59)
    and 8 more
    Cellular processes Cellular processes Detoxification Hg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 44.9)
    Signal transduction Regulatory functions DNA interactions Hg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 44.9)
    Signal transduction Regulatory functions DNA interactions Cd(II)/Pb(II)-responsive transcriptional regulator (TIGR02047; HMM-score: 44.5)
    Signal transduction Regulatory functions DNA interactions Cu(I)-responsive transcriptional regulator (TIGR02044; HMM-score: 40.5)
    Cellular processes Cellular processes Detoxification redox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 19.6)
    Signal transduction Regulatory functions DNA interactions redox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 19.6)
    cxxc_20_cxxc protein (TIGR04104; HMM-score: 16.7)
    MJ0042 family finger-like domain (TIGR02098; HMM-score: 5.7)
  • TheSEED  :
    • Transcriptional regulator, MerR family
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Cobalt-zinc-cadmium resistance  Transcriptional regulator, MerR family
  • PFAM:
    HTH (CL0123) MerR_1; MerR HTH family regulatory protein (PF13411; HMM-score: 61.7)
    and 26 more
    MerR; MerR family regulatory protein (PF00376; HMM-score: 46.7)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 19.6)
    MerR-DNA-bind; MerR, DNA binding (PF09278; HMM-score: 18.8)
    RING (CL0229) C1_4; TFIIH C1-like domain (PF07975; HMM-score: 16.8)
    Zn_Beta_Ribbon (CL0167) HVO_2753_ZBP; Small zinc finger protein HVO_2753-like, Zn-binding pocket (PF07754; HMM-score: 15.8)
    Zn_ribbon_4; zinc-ribbon domain (PF13717; HMM-score: 15.4)
    Tbcl_zf (CL0839) DUF4379; Probable Treble clef zinc finger domain (PF14311; HMM-score: 14.8)
    no clan defined RAC_head; Ribosome-associated complex head domain (PF16717; HMM-score: 14.5)
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 14)
    Zn_ribbon_20; Zinc beta-ribbon domain (PF23551; HMM-score: 13.9)
    C2H2-zf (CL0361) zf-Di19; Drought induced 19 protein (Di19), zinc-binding (PF05605; HMM-score: 12.1)
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_16; Zinc beta-ribbon finger, putative (PF21957; HMM-score: 11.9)
    Zn_ribbon_10; ER junction formation factor lunapark, zinc ribbon finger (PF10058; HMM-score: 11.4)
    no clan defined Opy2; Opy2 protein (PF09463; HMM-score: 10.9)
    Zn_Beta_Ribbon (CL0167) Cas12f1-like_TNB; Cas12f1-like, TNB domain (PF07282; HMM-score: 10.8)
    Zn_ribbon_FGT1_1; FORGETTER1 first zinc ribbon domain (PF23547; HMM-score: 10.6)
    Zn_ribbon_TFIIS; Transcription factor S-II (TFIIS) (PF01096; HMM-score: 10.3)
    Zn_ribbon_Nudix; Nudix N-terminal (PF14803; HMM-score: 10.3)
    Tbcl_zf (CL0839) zf_Rg; Reverse gyrase zinc finger (PF17915; HMM-score: 10.2)
    no clan defined SlyX; SlyX (PF04102; HMM-score: 10.1)
    DUF7385; Family of unknown function (DUF7385) (PF24110; HMM-score: 8.9)
    C2H2-zf (CL0361) zf-H2C2_2; Zinc-finger double domain (PF13465; HMM-score: 8.6)
    Zn_Beta_Ribbon (CL0167) DUF7560; Family of unknown function (DUF7560) (PF24441; HMM-score: 8.1)
    CpXC; CpXC protein (PF14353; HMM-score: 7)
    Zn_ribbon_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 6.8)
    no clan defined YnfU; YnfU (PF23499; HMM-score: 6.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0146
    • Cytoplasmic Membrane Score: 0.9567
    • Cell wall & surface Score: 0.0032
    • Extracellular Score: 0.0255
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004422
    • TAT(Tat/SPI): 0.000282
    • LIPO(Sec/SPII): 0.000685
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTEQQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLNKIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIMSSILMKNKWPFLIALPFMNCYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATTRRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA2296&oldid=86278"