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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2403 [new locus tag: SACOL_RS12610 ]
  • pan locus tag?: SAUPAN005938000
  • symbol: SACOL2403
  • pan gene symbol?: —
  • synonym:
  • product: ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2403 [new locus tag: SACOL_RS12610 ]
  • symbol: SACOL2403
  • product: ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2464671..2466221
  • length: 1551
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3236038 NCBI
  • RefSeq: YP_187206 NCBI
  • BioCyc: see SACOL_RS12610
  • MicrobesOnline: 913883 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAAAAGAAATTAGGTATGTTACTTCTTGTACCAGCCGTAACTTTATCATTAGCCGCA
    TGTGGGAATGATGATGGAAAAGATAAAGATGGCAAGGTAACAATTAAAACGACAGTTTAT
    CCATTGCAATCATTTGCAGAGCAAATTGGTGGAAAACACGTGAAGGTATCATCAATCTAT
    CCAGCAGGGACAGATTTACATAGCTATGAACCAACACAAAAAGATATATTAAGTGCAAGC
    AAGTCAGACTTGTTTATGTATACAGGGGATAATTTAGATCCGGTTGCTAAGAAAGTTGCA
    TCTACTATCAAAGATAAAGATAAAAAACTGTCTTTAGAGGATAAATTAGATAAAGCAAAG
    CTTTTAACTGATCAACACGAGCATGGTGAAGAGCATGAACATGAGGGACATGATCATGAG
    AAAGAAGAACATCATCATCATCATGGTGGATATGATCCACACGTATGGTTAGATCCTAAA
    ATTAACCAAACTTTCGCTAAAGAAATTAAAGATGAATTAGTGAAAAAAGATCCAAAACAT
    AAAGATGACTATGAGAAAAACTACAAAAAATTAAACGACGATCTTAAGAAAATTGATAAC
    GATATGAAGCAAGTTACAAAAGATAAGCAAGGTAATGCAGTATTCATTTCACATGAATCA
    ATTGGATACTTAGCTGATTGTTATGGTTTTGTTCAAAAAGGTATTCAAAACATGAATGCT
    GAAGATCCATCACAAAAAGAATTAACTAAAATTGTTAAAGAAATTAGAGATAGCAATGCT
    AAATATATTCTTTACGAAGATAATGTTGCGAATAAAGTGACTGAAACAATTCGTAAAGAA
    ACAGATGCGAAGCCTTTAAAATTCTACAACATGGAGTCTTTAAATAAAGAACAACAGAAA
    AAAGATAATATTACGTATCAATCATTAATGAAATCTAATATTGAAAATATCGGTAAAGCT
    TTAGACAGTGGTGTTAAAGTGAAAGACGACAAAGCTGAAAGTAAACATGACAAAGCAATT
    TCTGATGGGTATTTTAAAGATGAGCAAGTTAAAGACCGTGAATTAAGCGATTATGCTGGT
    GAATGGCAATCTGTTTACCCTTACTTAAAAGACGGTACGCTTGATGAAGTGATGGAACAT
    AAAGCTGAAAATGATCCGAAGAAATCTGCTAAAGATTTAAAAGCTTATTATGACAAAGGA
    TATAAAACTGATATTACTAACATTGATATAAAAGGAAATGAAATTACATTTACTAAAGAT
    GGTAAGAAACACACTGGTAAATATGAATACAATGGTAAGAAAACATTGAAATATCCTAAA
    GGTAACCGTGGCGTGAGATTTATGTTTAAATTGGTCGATGGTAATGATAAAGACTTACCG
    AAATTCATCCAATTTAGCGATCACAACATTGCACCTAAAAAGGCAGAACACTTCCATATC
    TTTATGGGTAATGATAATGACGCGTTATTAAAAGAAATGGATAACTGGCCAACATATTAT
    CCTTCAAAATTAAATAAAGACCAAATCAAAGAAGAAATGTTAGCGCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1551

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2403 [new locus tag: SACOL_RS12610 ]
  • symbol: SACOL2403
  • description: ABC transporter substrate-binding protein
  • length: 516
  • theoretical pI: 7.05056
  • theoretical MW: 59186.2
  • GRAVY: -1.03857

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate anchored repeat ABC transporter, substrate-binding protein (TIGR03772; HMM-score: 87.1)
  • TheSEED  :
    • Metal-binding protein ZinT
    • Zinc ABC transporter, substrate-binding AdcA
  • ⊞PFAM:
    Calycin (CL0116) ZinT; ZinT (YodA) periplasmic lipocalin-like zinc-recruitment (PF09223; HMM-score: 275.8)
    Chelatase (CL0043) ZnuA; Zinc-uptake complex component A periplasmic (PF01297; HMM-score: 236.6)
    and 7 more
    no clan defined DUF4971; Domain of unknown function (DUF4971) (PF16341; HMM-score: 16.9)
    LPD3; Large polyvalent protein-associated domain 3 (PF18798; HMM-score: 14.3)
    DUF4845; Domain of unknown function (DUF4845) (PF16137; HMM-score: 14)
    RNase_H (CL0219) Terminase-T7_RNaseH-like; Terminase Bacteriophage T7, Ribonuclease H-like domain (PF22530; HMM-score: 13.7)
    no clan defined DUF3107; Protein of unknown function (DUF3107) (PF11305; HMM-score: 13.5)
    sPC4_like (CL0609) T4_y05I_C; y05I-like putative transcription factor C-terminal domain (PF24448; HMM-score: 9.8)
    no clan defined Hia_Tpr_ring_dom; Trimeric autotransporter adhesin, Trp ring domain (PF22414; HMM-score: 9.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0007
    • Cytoplasmic Membrane Score: 0.8613
    • Cell wall & surface Score: 0.0709
    • Extracellular Score: 0.0671
  • ⊞LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: LSLAACG
  • ⊞SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.00062
    • TAT(Tat/SPI): 0.000068
    • LIPO(Sec/SPII): 0.999149
    • Cleavage Site: CS pos: 20-21. LAA-CG. Pr: 0.9997
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 57650948 NCBI
  • RefSeq: YP_187206 NCBI
  • UniProt: A0A0H2WXZ6 UniProt

⊟Protein sequence[edit | edit source]

  • MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQSFAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVASTIKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPKINQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETIRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESKHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDLKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKLVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQIKEEMLAH

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

  • ⊞regulator: Zur* (repression) regulon
    Zur*(TF)important in Zinc homeostasis; RegPrecise 

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL2403&oldid=79295"
  • This page was last edited on 11 March 2016, at 00:27.
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