PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2306 [new locus tag: NWMN_RS13255 ]
• pan locus tag^?: SAUPAN005938000
• symbol: NWMN_2306
• pan gene symbol^?: —
• synonym:
• product: Zn-binding lipoprotein adcA-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2306 [new locus tag: NWMN_RS13255 ]
• symbol: NWMN_2306
• product: Zn-binding lipoprotein adcA-like protein
• replicon: chromosome
• strand: -
• coordinates: 2533123..2534718
• length: 1596
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331412 NCBI
• RefSeq: YP_001333340 NCBI
• BioCyc:
• MicrobesOnline: 3707908 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAAAAAGAAATTAGGTATGTTACTTCTTGTACCAGCCGTAACTTTATCATTAGCCGCA
  TGTGGGAATGATGATGGAAAAGATAAAGATGGCAAGGTAACAATTAAAACGACAGTTTAT
  CCATTGCAATCATTTGCAGAGCAAATTGGTGGAAAACACGTGAAGGTATCATCAATCTAT
  CCAGCAGGGACAGATTTACATAGCTATGAACCAACACAAAAAGATATATTAAGTGCAAGC
  AAGTCAGACTTGTTTATGTATACAGGGGATAATTTAGATCCGGTTGCTAAGAAAGTTGCA
  TCTACTATCAAAGATAAAGATAAAAAACTGTCTTTAGAGGATAAATTAGATAAAGCAAAG
  CTTTTAACTGATCAACACGAGCATGGTGAAGAGCATGAACATGAGGGACATGATCATGAG
  AAAGAAGAACATCATCATCATCATGGTGGATATGATCCACACGTATGGTTAGATCCTAAA
  ATTAACCAAACTTTCGCTAAAGAAATTAAAGATGAATTAGTGAAAAAAGATCCAAAACAT
  AAAGATGACTATGAGAAAAACTACAAAAAATTAAACGACGATCTTAAGAAAATTGATAAC
  GATATGAAGCAAGTTACAAAAGATAAGCAAGGTAATGCAGTATTCATTTCACATGAATCA
  ATTGGATACTTAGCTGATTGTTATGGTTTTGTTCAAAAAGGTATTCAAAACATGAATGCT
  GAAGATCCATCACAAAAAGAATTAACTAAAATTGTTAAAGAAATTAGAGATAGCAATGCT
  AAATATATTCTTTACGAAGATAATGTTGCGAATAAAGTGACTGAAACAATTCGTAAAGAA
  ACAGATGCGAAGCCTTTAAAATTCTACAACATGGAGTCTTTAAATAAAGAACAACAGAAA
  AAAGATAATATTACGTATCAATCATTAATGAAATCTAATATTGAAAATATCGGTAAAGCT
  TTAGACAGTGGTGTTAAAGTGAAAGACGACAAAGCTGAAAGTAAACATGACAAAGCAATT
  TCTGATGGGTATTTTAAAGATGAGCAAGTTAAAGACCGTGAATTAAGCGATTATGCTGGT
  GAATGGCAATCTGTTTACCCTTACTTAAAAGACGGTACGCTTGATGAAGTGATGGAACAT
  AAAGCTGAAAATGATCCGAAGAAATCTGCTAAAGATTTAAAAGCTTATTATGACAAAGGA
  TATAAAACTGATATTACTAACATTGATATAAAAGGAAATGAAATTACATTTACTAAAGAT
  GGTAAGAAACACACTGGTAAATATGAATACAATGGTAAGAAAACATTGAAATATCCTAAA
  GGTAACCGTGGCGTGAGATTTATGTTTAAATTGGTCGATGGTAATGATAAAGACTTACCG
  AAATTCATCCAATTTAGCGATCACCACATTGCACCTAAAAAGGCAGAACACTTCCATATC
  TTTATGGGTAATGATAATGACGCGTTATTAAAAAGAATGGATTACTGGCCAACATATTAT
  CCTTCAAAATTAAATAAAGACCCAATCAAAGAAGAAATGTTAGCGCATTATTTCAACAGG
  TTTGCACACTCTACTCAACTGTTAATAGCTGAATAG
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⊟Protein

⊟General

• locus tag: NWMN_2306 [new locus tag: NWMN_RS13255 ]
• symbol: NWMN_2306
• description: Zn-binding lipoprotein adcA-like protein
• length: 531
• theoretical pI: 7.33174
• theoretical MW: 61046.4
• GRAVY: -1.00226

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Unknown substrate anchored repeat ABC transporter, substrate-binding protein (TIGR03772; HMM-score: 87)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Calycin (CL0116) ZinT; ZinT (YodA) periplasmic lipocalin-like zinc-recruitment (PF09223; HMM-score: 261.3)
  Chelatase (CL0043) ZnuA; Zinc-uptake complex component A periplasmic (PF01297; HMM-score: 221.5)
  and 4 more
  no clan defined DUF4845; Domain of unknown function (DUF4845) (PF16137; HMM-score: 17.1)
  DUF4971; Domain of unknown function (DUF4971) (PF16341; HMM-score: 15.3)
  DUF3107; Protein of unknown function (DUF3107) (PF11305; HMM-score: 13.6)
  DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 6.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.68
  • Cellwall Score: 0.17
  • Extracellular Score: 0.16
  • Internal Helices: 0
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: LSLAACG
• SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.00062
  • TAT(Tat/SPI): 0.000068
  • LIPO(Sec/SPII): 0.999149
  • Cleavage Site: CS pos: 20-21. LAA-CG. Pr: 0.9997
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222518 NCBI
• RefSeq: YP_001333340 NCBI
• UniProt: A0A0H3KA96 UniProt

⊟Protein sequence

• MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQSFAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVASTIKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPKINQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETIRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESKHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDLKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKLVDGNDKDLPKFIQFSDHHIAPKKAEHFHIFMGNDNDALLKRMDYWPTYYPSKLNKDPIKEEMLAHYFNRFAHSTQLLIAE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: Zur* (repression) regulon

  Zur*

  (TF)

  important in Zinc homeostasis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications