PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0980 [new locus tag: SA_RS05555 ]
pan locus tag^?: SAUPAN003363000
symbol: isdE
pan gene symbol^?: isdE
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0980 [new locus tag: SA_RS05555 ]
• symbol: isdE
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1109815..1110693
• length: 879
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123807 NCBI
• RefSeq: NP_374250 NCBI
• BioCyc: see SA_RS05555
• MicrobesOnline: 103276 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  TTGAGAATCATAAAGTATTTAACCATTTTAGTGATAAGCGTCGTTATCTTAACCAGCTGT
  CAATCTTCCAGTTCTCAAGAATCAACTAAATCCGGCGAATTCAGAATCGTACCAACAACT
  GTTGCATTGACAATGACATTGGACAAATTGGATTTACCAATTGTCGGCAAACCCACGTCA
  TATAAGACATTGCCTAATCGTTATAAAGATGTACCGGAAATTGGTCAACCAATGGAGCCG
  AATGTTGAAGCTGTTAAAAAGTTAAAACCAACACATGTTTTGAGTGTGTCAACGATTAAA
  GATGAAATGCAACCATTTTACAAACAATTAAATATGAAAGGCTACTTTTATGATTTTGAT
  AGTTTAAAAGGGATGCAAAAGTCGATTACACAATTAGGTGATCAATTTAATCGTAAAGCA
  CAAGCAAAAGAATTAAATGACCATTTAAATTCTGTAAAGCAAAAAATTGAAAATAAAGCA
  GCTAAACAAAAGAAACATCCCAAAGTATTAATATTAATGGGTGTACCGGGTAGCTATTTA
  GTAGCAACTGATAAATCATATATTGGTGATTTAGTTAAAATAGCAGGTGGAGAAAATGTT
  ATTAAAGTGAAAGATCGTCAATATATTTCGTCTAATACTGAAAATTTGTTGAATATCAAT
  CCAGATATTATTTTACGATTACCACACGGAATGCCTGAAGAAGTTAAGAAAATGTTTCAA
  AAAGAATTTAAACAAAATGATATTTGGAAACATTTTAAAGCTGTGAAAAATAATCATGTT
  TATGACTTAGAGGAAGTGCCATTCGGTATTACAGCAAATGTTGATGCTGATAAGGCAATG
  ACTCAATTATATGATTTATTTTATAAGGATAAAAAATAG
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⊟Protein

• 2Q8P
  PDB
• 2Q8Q
  PDB

Protein Data Bank: 2Q8Q

⊟General

• locus tag: SA0980 [new locus tag: SA_RS05555 ]
• symbol: IsdE
• description: hypothetical protein
• length: 292
• theoretical pI: 9.91166
• theoretical MW: 33270.5
• GRAVY: -0.440753

⊟Function

• ⊞TIGRFAM:
  heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 411.6)
  and 3 more
  putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 43.7)
  Cell structure Cell envelope Other uncharacterized lipoprotein (TIGR02722; HMM-score: 13.1)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking twin arginine-targeting protein translocase TatB (TIGR01410; HMM-score: 7.5)
• TheSEED  :
  • Heme transporter IsdDEF, lipoprotein IsdE
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme transporter IsdDEF, lipoprotein IsdE
• ⊞PFAM:
  Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 144.4)
  and 5 more
  no clan defined Muted; Organelle biogenesis, Muted-like protein (PF14942; HMM-score: 18.2)
  HAD (CL0137) NT5C; 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C) (PF06941; HMM-score: 15.9)
  no clan defined DUF2098; Uncharacterized protein conserved in archaea (DUF2098) (PF09871; HMM-score: 14.9)
  GDH2_N; NAD-dependent glutamate dehydrogenase, N-terminal domain (PF23147; HMM-score: 14.5)
  TIM_barrel (CL0036) ICL; Isocitrate lyase family (PF00463; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: heme b
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.93
  • Cell wall & surface Score: 0.0014
  • Extracellular Score: 0.0685
• ⊞LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
  • SP(Sec/SPI): 0.000789
  • TAT(Tat/SPI): 0.000052
  • LIPO(Sec/SPII): 0.999024
  • Cleavage Site: CS pos: 19-20. LTS-CQ. Pr: 0.9994
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926717 NCBI
• RefSeq: NP_374250 NCBI
• UniProt: Q7A652 UniProt

⊟Protein sequence

• MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALTMTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDEMQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKAAKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLNINPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDADKAMTQLYDLFYKDKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• isdC > isdD > isdE > isdF > srtB > isdG  

⊟Regulation

• ⊞regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Jason C Grigg, Christie L Vermeiren, David E Heinrichs, Michael E P Murphy
Heme coordination by Staphylococcus aureus IsdE.
J Biol Chem: 2007, 282(39);28815-22
[PubMed:17666394] [WorldCat.org] [DOI] (P p)