⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS07290 [old locus tag: SA1287 ]
pan locus tag^?: SAUPAN003914000
symbol: asnC
pan gene symbol^?: asnC
• synonym:
• product: asparagine--tRNA ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS07290 [old locus tag: SA1287 ]
• symbol: asnC
• product: asparagine--tRNA ligase
• replicon: chromosome
• strand: -
• coordinates: 1491414..1492706
• length: 1293
• essential: yes DEG other strains

⊟Accession numbers

• Location: NC_002745 (1491414..1492706) NCBI
• BioCyc: SA_RS07290 BioCyc
• MicrobesOnline: see SA1287

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAACAACGATTAAACAAGCGAAAGATCATTTAAACCAAGACGTTACAATTGGTGCT
  TGGTTAACAAATAAACGTTCAAGTGGTAAAATCGCCTTTTTACAATTACGTGATGGAACA
  GGCTTTATGCAAGGCGTAGTAGTTAAATCAGAAGTTGATGAAGAGGTATTCAAACTTGCG
  AAAGAAATTGCTCAAGAATCATCTCTATACGTTACAGGCACAATTACAGAAGATAATCGT
  TCTGACTTAGGATACGAAATGCAAGTGAAATCAATTGAAGTTATTTCAGAAGCGCATGAC
  TATCCGATTACACCTAAAAATCATGGTACAGAATTCTTAATGGATCACCGTCATTTATGG
  TTACGTTCTAAAAAACAACATGCTGTAATGAAAATTAGAAATGAAGTTATTCGTGCAACG
  TATGAATTTTTCAACAAAGATGGATTTACAAAGGTTGATCCACCAATTTTGACAGCAAGT
  GCACCAGAAGGTACAAGTGAATTATTCCATACTAAATACTTTGATCAAGATGCGTTTTTA
  TCTCAAAGTGGTCAGTTATACTTAGAAGCTGCAGCAATGGCACACGGAAAAGTATTTTCA
  TTTGGTCCAACTTTCAGAGCTGAAAAATCAAAAACACGTAGACACTTGATCGAGTTCTGG
  ATGATTGAAGGGGAAATGGCTTTCACAAATCATGCTGAAAGTTTAGAAATTCAAGAACAA
  TATGTAACACATGTAGTAAAATCAGTTTTAGAAAATTGTAAACTAGAGTTGAAAATTTTA
  GAGCGTGATACATCAAAACTTGAAAAAGTTGCGACACCATTCCCTAGAATTTCATATGAT
  GATGCAATTGAATTCTTAAAAGCAGAAGGCTTTGATGATATTGAATGGGGTGAAGATTTT
  GGTGCGCCACATGAAACTGCCATTGCTAATCATTATGATTTACCGGTGTTTATTACTAAT
  TATCCAACTAAAATTAAGCCTTTCTATATGCAACCAAATCCTGAGAATGAAGAAACTGTC
  TTATGTGCAGACTTAATTGCACCTGAAGGATACGGTGAAATTATCGGTGGATCTGAACGT
  GTGGATGACTTAGAATTGTTAGAACAACGCGTTAAAGAACATGGATTAGACGAAGAAGCA
  TATAGTTACTACTTAGACTTACGTCGTTATGGTAGTGTGCCACACTGTGGATTTGGTTTA
  GGTTTAGAGCGCACAGTAGCATGGATTTCTGGTGTTGAACACGTTCGTGAAACTGCGCCA
  TTCCCAAGATTATTAAACCGTTTATATCCATAA
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⊟Protein

⊟General

• locus tag: SA_RS07290 [old locus tag: SA1287 ]
• symbol: AsnC
• description: asparagine--tRNA ligase
• length: 430
• theoretical pI: 5.09478
• theoretical MW: 49127.2
• GRAVY: -0.437209

⊟Function

• ⊞⊞reaction:
  EC 6.1.1.22?  ExPASy
  Asparagine--tRNA ligase ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn)
• ⊞⊞TIGRFAM:
  Genetic information processing Protein synthesis tRNA aminoacylation asparagine--tRNA ligase (TIGR00457; EC 6.1.1.22; HMM-score: 545.3)
  and 6 more
  Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA(Asn) ligase (TIGR00458; EC 6.1.1.23; HMM-score: 336.6)
  Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA ligase (TIGR00459; EC 6.1.1.-; HMM-score: 154.4)
  Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00499; EC 6.1.1.6; HMM-score: 97.5)
  Unknown function General EF-P lysine aminoacylase GenX (TIGR00462; HMM-score: 34.4)
  Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 22.1)
  Metabolism Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 11.4)
• TheSEED: see SA1287
• ⊞⊞PFAM:
  tRNA_synt_II (CL0040) tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 248.7)
  and 3 more
  OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 65.5)
  tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 16.8)
  tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 15.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9984
  • Cytoplasmic Membrane Score: 0
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0016
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004198
  • TAT(Tat/SPI): 0.000436
  • LIPO(Sec/SPII): 0.000634
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446781523 NCBI
• RefSeq: WP_000858779 NCBI
• UniProt: see SA1287

⊟Protein sequence

• MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQGVVVKSEVDEEVFKLAKEIAQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYPITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTASAPEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIEFWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPRISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNPENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGSVPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• ⊟⊟interaction partners:

  SA_RS08545		isocitrate dehydrogenase (NADP(+))   (data from MRSA252)
  SA_RS08560		pyruvate kinase   (data from MRSA252)
  SA_RS11430		Asp23/Gls24 family envelope stress response protein   (data from MRSA252)
  SA_RS11720		30S ribosomal protein S17   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications