From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1494 [new locus tag: SACOL_RS07610 ]
  • pan locus tag?: SAUPAN003914000
  • symbol: asnC
  • pan gene symbol?: asnC
  • synonym:
  • product: asparaginyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1494 [new locus tag: SACOL_RS07610 ]
  • symbol: asnC
  • product: asparaginyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1532975..1534267
  • length: 1293
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAAACAACGATTAAACAAGCGAAAGATCATTTAAACCAAGACGTTACAATTGGTGCT
    TGGTTAACAAATAAACGTTCAAGTGGTAAAATCGCCTTTTTACAATTACGTGATGGAACA
    GGCTTTATGCAAGGCGTAGTAGTTAAATCAGAAGTTGATGAAGAGGTATTCAAACTTGCG
    AAAGAAATTACTCAAGAATCATCTCTATACGTTACAGGCACAATTACAGAAGATAATCGT
    TCTGACTTAGGATACGAAATGCAAGTGAAATCAATTGAAGTTATTTCAGAAGCGCATGAC
    TATCCGATTACACCTAAAAATCATGGTACAGAATTCTTAATGGATCACCGTCATTTATGG
    TTACGTTCTAAAAAACAACATGCTGTAATGAAAATTAGAAATGAAGTTATTCGTGCAACG
    TATGAATTTTTCAACAAAGATGGATTTACAAAGGTTGATCCACCAATTTTGACAGCAAGT
    GCGCCAGAAGGTACAAGTGAATTATTCCATACTAAATACTTTGATCAAGATGCGTTTTTA
    TCTCAAAGTGGTCAGTTATACTTAGAAGCTGCAGCAATGGCACACGGAAAAGTATTTTCA
    TTTGGTCCAACTTTCAGAGCTGAAAAATCAAAAACACGTAGACACTTGATCGAGTTCTGG
    ATGATTGAAGGAGAAATGGCTTTCACAAATCATGCTGAAAGTTTAGAAATTCAAGAACAA
    TATGTAACACATGTTGTAAAATCAGTTTTAGAAAATTGTAAACTAGAGTTGAAAATTTTA
    GAGCGTGATACATCAAAACTTGAAAAAGTTGCGACACCATTCCCTAGAATTTCATATGAT
    GATGCAATTGAATTCTTAAAAGCAGAAGGCTTTGATGATATTGAATGGGGCGAAGATTTT
    GGTGCGCCACATGAAACAGCCATTGCTAATCATTATGATTTACCGGTGTTTATTACTAAT
    TATCCAACTAAAATTAAGCCTTTCTATATGCAACCAAATCCTGAAAATGAAGAAACTGTA
    TTATGTGCAGACTTAATTGCACCTGAAGGATACGGTGAAATTATTGGTGGATCTGAACGT
    GTGGATGACTTAGAATTGTTAGAACAACGCGTTAAAGAACATGGATTAGACGAAGAAGCA
    TATAGTTACTACTTAGACTTACGTCGTTATGGTAGTGTGCCACACTGTGGATTTGGTTTA
    GGTTTAGAGCGTACAGTAGCATGGATTTCTGGTGTTGAACACGTTCGTGAAACAGCGCCA
    TTCCCAAGATTATTAAACCGTTTATATCCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1293

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1494 [new locus tag: SACOL_RS07610 ]
  • symbol: AsnC
  • description: asparaginyl-tRNA synthetase
  • length: 430
  • theoretical pI: 5.09478
  • theoretical MW: 49157.2
  • GRAVY: -0.443023

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.22?  ExPASy
    Asparagine--tRNA ligase ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation asparagine--tRNA ligase (TIGR00457; EC 6.1.1.22; HMM-score: 546.9)
    and 6 more
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA(Asn) ligase (TIGR00458; EC 6.1.1.23; HMM-score: 336.8)
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA ligase (TIGR00459; EC 6.1.1.-; HMM-score: 156.2)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00499; EC 6.1.1.6; HMM-score: 97.8)
    Unknown function General EF-P lysine aminoacylase GenX (TIGR00462; HMM-score: 34.4)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 22.1)
    Metabolism Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 11.3)
  • TheSEED  :
    • Asparaginyl-tRNA synthetase (EC 6.1.1.22)
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Glu and Gln  Asparaginyl-tRNA synthetase (EC 6.1.1.22)
    and 1 more
    RNA Metabolism RNA processing and modification tRNA modification Archaea  Asparaginyl-tRNA synthetase (EC 6.1.1.22)
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 278.6)
    and 5 more
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 59.7)
    tRNA_synt_II (CL0040) tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 17.9)
    tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 15.8)
    no clan defined YliH; Biofilm formation protein (YliH/bssR) (PF10799; HMM-score: 13.1)
    OB (CL0021) tRNA_anti_2; OB-fold nucleic acid binding domain (PF13742; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004447
    • TAT(Tat/SPI): 0.000467
    • LIPO(Sec/SPII): 0.000662
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQGVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYPITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTASAPEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIEFWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPRISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNPENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGSVPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 772 [5]
  • interaction partners:
    SACOL1741(icd)isocitrate dehydrogenase  [6] (data from MRSA252)
    SACOL1745(pyk)pyruvate kinase  [6] (data from MRSA252)
    SACOL2230(rpsQ)30S ribosomal protein S17  [6] (data from MRSA252)
    SACOL2173alkaline shock protein 23  [6] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ 6.0 6.1 6.2 6.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL1494&oldid=98830"