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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1448 [new locus tag: NWMN_RS08170 ]
  • pan locus tag?: SAUPAN004123000
  • symbol: NWMN_1448
  • pan gene symbol?: comGA
  • synonym:
  • product: competence protein ComGA-like protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1448 [new locus tag: NWMN_RS08170 ]
  • symbol: NWMN_1448
  • product: competence protein ComGA-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1616115..1617089
  • length: 975
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 5330862 NCBI
  • RefSeq: YP_001332482 NCBI
  • BioCyc:
  • MicrobesOnline: 3707000 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTA
    CATTTTATTCCAGTTAAAAATGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAG
    CAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTATTAGTTTATATGAAGTTTCAA
    GCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
    AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGT
    GTTATCAGAATTGTACCTCAATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGAT
    TTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGTTTAGTGGACCAACTGGTTCA
    GGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
    GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTG
    AATGATAAAGCTGGCATAAACTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCT
    GATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTGCCAAGTGTGTTATACAGGCT
    AGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
    TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATT
    ATAAACCAACGACTTGTAACTACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTA
    TCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATTCATTACCATCATCATTTAAG
    AACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
    CATAAATACATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1448 [new locus tag: NWMN_RS08170 ]
  • symbol: NWMN_1448
  • description: competence protein ComGA-like protein
  • length: 324
  • theoretical pI: 9.07987
  • theoretical MW: 36864.8
  • GRAVY: -0.0317901

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cellular processes Cellular processes Pathogenesis type II secretion system protein E (TIGR02533; HMM-score: 221.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein E (TIGR02533; HMM-score: 221.8)
    Cell structure Cell envelope Surface structures type IV-A pilus assembly ATPase PilB (TIGR02538; HMM-score: 213.5)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type IV-A pilus assembly ATPase PilB (TIGR02538; HMM-score: 213.5)
    and 14 more
    Cell structure Cell envelope Surface structures twitching motility protein (TIGR01420; HMM-score: 146.5)
    Cellular processes Cellular processes Chemotaxis and motility twitching motility protein (TIGR01420; HMM-score: 146.5)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid transfer ATPase TraJ (TIGR02525; HMM-score: 84.9)
    Dot/Icm secretion system ATPase DotB (TIGR02524; HMM-score: 58.2)
    Cellular processes Cellular processes Conjugation P-type conjugative transfer ATPase TrbB (TIGR02782; HMM-score: 57.5)
    P-type DNA transfer ATPase VirB11 (TIGR02788; HMM-score: 54.5)
    helicase/secretion neighborhood ATPase (TIGR03819; HMM-score: 35.9)
    Cellular processes Cellular processes Sporulation and germination stage III sporulation protein AA (TIGR02858; HMM-score: 17.1)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 14.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 14.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 14.1)
    Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 12.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 12.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 12.2)
  • ⊞TheSEED  :
    • Late competence protein ComGA, access of DNA to ComEA
    DNA Metabolism DNA uptake, competence Gram Positive Competence  Late competence protein ComGA, access of DNA to ComEA
    and 1 more
    DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComGA, access of DNA to ComEA
  • ⊞PFAM:
    P-loop_NTPase (CL0023) T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 251.4)
    and 21 more
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 25.7)
    nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 19.7)
    Rad17; Rad17 P-loop domain (PF03215; HMM-score: 18.7)
    DAP3; Mitochondrial ribosomal death-associated protein 3 (PF10236; HMM-score: 18.2)
    AAA_7; P-loop containing dynein motor region (PF12775; HMM-score: 18)
    AAA_14; AAA domain (PF13173; HMM-score: 17.6)
    NPHP3_N; Nephrocystin 3, N-terminal (PF24883; HMM-score: 17.2)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.3)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 16.2)
    AAA_23; AAA domain (PF13476; HMM-score: 15.9)
    AAA_22; AAA domain (PF13401; HMM-score: 15.2)
    ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 15.1)
    AAA_30; AAA domain (PF13604; HMM-score: 14.9)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 14.4)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 14.4)
    G-alpha; G-protein alpha subunit (PF00503; HMM-score: 14)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 13.2)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 13.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 13)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 12.5)
    AAA_25; AAA domain (PF13481; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.6324
    • Cytoplasmic Membrane Score: 0.3537
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0139
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003912
    • TAT(Tat/SPI): 0.000073
    • LIPO(Sec/SPII): 0.000327
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 151221660 NCBI
  • RefSeq: YP_001332482 NCBI
  • UniProt: A0A0H3K8R1 UniProt

⊟Protein sequence[edit | edit source]

  • MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLNVISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: NWMN_1443 < NWMN_1444 < NWMN_1445 < NWMN_1446 < NWMN_1447 < NWMN_1448 < NWMN_1449 < NWMN_1450 < glk

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_1448&oldid=64306"
  • This page was last edited on 10 March 2016, at 17:26.
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