PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

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⊟Summary

• organism: Staphylococcus aureus NCTC8325
locus tag: SAOUHSC_00471
pan locus tag^?: SAUPAN002239000
symbol: glmU
pan gene symbol^?: glmU
• synonym:
• product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAOUHSC_00471
• symbol: glmU
• product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase
• replicon: chromosome
• strand: +
• coordinates: 469575..470927
• length: 1353
• essential: yes  DEG other strains

⊟Accession numbers

• Gene ID: 3920331 NCBI
• RefSeq: YP_499050 NCBI
• BioCyc: G1I0R-437 BioCyc
• MicrobesOnline: 1288948 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCGAAGACACGCGATAATTTTGGCAGCAGGTAAAGGCACAAGAATGAAATCTAAAAAG
  TATAAAGTGCTACACGAGGTTGCTGGGAAACCTATGGTCGAACATGTATTGGAAAGTGTG
  AAAGGCTCTGGTGTCGATCAAGTTGTAACCATCGTAGGACATGGTGCTGAAAGTGTAAAA
  GGACATTTAGGCGAGCGTTCTTTATACAGTTTTCAAGAGGAACAACTCGGTACTGCGCAT
  GCAGTGCAAATGGCGAAATCACACTTAGAAGACAAGGAAGGTACGACAATCGTTGTATGT
  GGTGACACACCGCTCATCACAAAGGAAACATTAGTAACATTGATTGCGCATCACGAGGAT
  GCTAATGCTCAAGCAACTGTATTATCTGCATCGATTCAACAACCATATGGATACGGAAGA
  ATCGTTCGAAATGCGTCAGGTCGTTTAGAACGCATAGTTGAAGAGAAAGATGCAACGCAA
  GCTGAAAAGGATATTAATGAAATTAGTTCAGGTATTTTTGCGTTTAATAATAAAACGTTG
  TTTGAAAAATTAACACAAGTGAAAAATGATAATGCGCAAGGTGAATATTACCTCCCTGAT
  GTATTGTCGTTAATTTTAAATGATGGCGGCATCGTAGAAGTCTATCGTACCAATGATGTT
  GAAGAAATCATGGGTGTAAATGATCGTGTAATGCTTAGTCAGGCTGAGAAGGCGATGCAA
  CGTCGTACGAATCATTATCACATGCTAAATGGTGTGACAATCATCGATCCTGACAGCACT
  TATATTGGTCCAGACGTTACAATTGGTAGTGATACAGTCATTGAACCAGGCGTACGAATT
  AATGGTCGTACAGAAATTGGCGAAGATGTTGTTATTGGTCAGTACTCTGAAATTAACAAT
  AGTACGATTGAAAATGGTGCATGTATTCAACAGTCTGTTGTTAATGATGCTAGCGTAGGA
  GCGAATACTAAGGTCGGACCGTTTGCGCAATTGAGACCAGGCGCGCAATTAGGTGCAGAT
  GTTAAGGTTGGAAATTTTGTAGAAATTAAAAAAGCAGATCTTAAAGATGGTGCCAAGGTT
  TCACATTTAAGTTATATTGGCGATGCTGTAATTGGCGAACGTACTAATATTGGTTGCGGA
  ACGATTACAGTTAACTATGATGGTGAAAATAAATTTAAAACTATCGTCGGCAAAGATTCA
  TTTGTAGGTTGCAATGTTAATTTAGTAGCACCTGTAACAATTGGTGATGATGTATTGGTG
  GCAGCTGGTTCCACAATCACAGATGACGTACCAAATGACAGTTTAGCTGTGGCAAGAGCA
  AGACAAACAACAAAAGAAGGATATAGGAAATAA
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⊟Protein

⊟General

• locus tag: SAOUHSC_00471
• symbol: GlmU
• description: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase
• length: 450
• theoretical pI: 5.6261
• theoretical MW: 48502.4
• GRAVY: -0.248667

⊟Function

• ⊞reaction:
  EC 2.7.7.23?  ExPASy
  UDP-N-acetylglucosamine diphosphorylase UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine
  EC 2.3.1.157?  ExPASy
  Glucosamine-1-phosphate N-acetyltransferase Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
• ⊞TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 587.6)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 587.6)
  Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 587.6)
  and 25 more
  UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03992; EC 2.3.1.157,2.7.7.23; HMM-score: 204.4)
  glucose-1-phosphate thymidylyltransferase (TIGR01208; EC 2.7.7.24; HMM-score: 93)
  sugar O-acyltransferase, sialic acid O-acetyltransferase NeuD family (TIGR03570; HMM-score: 62.9)
  molybdenum cofactor cytidylyltransferase (TIGR03310; EC 2.7.7.76; HMM-score: 55.3)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glucose-1-phosphate adenylyltransferase (TIGR02091; EC 2.7.7.27; HMM-score: 43.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase (TIGR01852; EC 2.3.1.129; HMM-score: 41.9)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD (TIGR01853; EC 2.3.1.191; HMM-score: 41.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TIGR00453; EC 2.7.7.60; HMM-score: 40.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UTP--glucose-1-phosphate uridylyltransferase (TIGR01099; EC 2.7.7.9; HMM-score: 38.4)
  Hypothetical proteins Conserved TIGR00454 family protein (TIGR00454; HMM-score: 36.3)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides glucose-1-phosphate thymidylyltransferase (TIGR01207; EC 2.7.7.24; HMM-score: 36.2)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate cytidylyltransferase (TIGR00466; EC 2.7.7.38; HMM-score: 35.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor guanylyltransferase (TIGR02665; EC 2.7.7.77; HMM-score: 35.3)
  colanic acid biosynthesis acetyltransferase WcaB (TIGR04016; EC 2.3.1.-; HMM-score: 27.2)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03991; EC 2.3.1.157,2.7.7.23; HMM-score: 26.6)
  Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03991; EC 2.3.1.157,2.7.7.23; HMM-score: 26.6)
  phosphonate metabolim protein, transferase hexapeptide repeat family (TIGR03308; HMM-score: 26.5)
  2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (TIGR03532; EC 2.3.1.89; HMM-score: 26.1)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides glucose-1-phosphate cytidylyltransferase (TIGR02623; EC 2.7.7.33; HMM-score: 24.1)
  non-ribosomal peptide synthetase terminal domain of unknown function (TIGR02353; HMM-score: 20.9)
  Metabolism Amino acid biosynthesis Serine family serine O-acetyltransferase (TIGR01172; EC 2.3.1.30; HMM-score: 19.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 2-phospho-L-lactate guanylyltransferase (TIGR03552; EC 2.7.7.68; HMM-score: 17.4)
  Metabolism Energy metabolism Other phenylacetic acid degradation protein PaaY (TIGR02287; HMM-score: 17.3)
  xanthine dehydrogenase accessory protein pucB (TIGR03202; HMM-score: 17)
  colanic acid biosynthesis acetyltransferase WcaF (TIGR04008; EC 2.3.1.-; HMM-score: 16.5)
• ⊞TheSEED  :
  • Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)
  • N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23)
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)
  and 5 more
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23)
• ⊞PFAM:
  HEXAPEP (CL0536) Hexapep; Bacterial transferase hexapeptide (six repeats) (PF00132; HMM-score: 104.3)
  GT-A (CL0110) NTP_transferase; Nucleotidyl transferase (PF00483; HMM-score: 87.3)
  and 8 more
  NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 75.7)
  HEXAPEP (CL0536) LbH_EIF2B; EIF2B subunit epsilon LbH domain (PF25084; HMM-score: 49)
  GT-A (CL0110) IspD; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (PF01128; HMM-score: 36)
  CTP_transf_3; Cytidylyltransferase (PF02348; HMM-score: 35.2)
  HEXAPEP (CL0536) Hexapep_2; Hexapeptide repeat of succinyl-transferase (PF14602; HMM-score: 30.4)
  GMPPB_C; GMPPB C-terminal domain (PF25087; HMM-score: 22.5)
  Hexapep_GlmU; GlmU-like, C-terminal hexapeptide (PF24894; HMM-score: 17.7)
  GT-A (CL0110) CofC; Guanylyl transferase CofC like (PF01983; HMM-score: 14.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9956
  • Cytoplasmic Membrane Score: 0.0002
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0041
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01358
  • TAT(Tat/SPI): 0.000791
  • LIPO(Sec/SPII): 0.002605
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 88194258 NCBI
• RefSeq: YP_499050 NCBI
• UniProt: Q2G0S3 UniProt
• STRING: 93061.SAOUHSC_00471 STRING

⊟Protein sequence

• MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSGVDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGDTPLITKETLVTLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQAEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTNDVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEPGVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPGAQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKFKTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK

⊟Experimental data

• experimentally validated: PeptideAtlas
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• ⊟interaction partners:

  SAOUHSC_02500	(rplE)	50S ribosomal protein L5   (data from MRSA252)
  SAOUHSC_00518	(rplK)	50S ribosomal protein L11   (data from MRSA252)
  SAOUHSC_00521	(rplL)	50S ribosomal protein L7/L12   (data from MRSA252)
  SAOUHSC_01757	(rplU)	50S ribosomal protein L21   (data from MRSA252)
  SAOUHSC_02494	(rpsE)	30S ribosomal protein S5   (data from MRSA252)
  SAOUHSC_02477	(rpsI)	30S ribosomal protein S9   (data from MRSA252)
  SAOUHSC_00187		formate acetyltransferase   (data from MRSA252)
  SAOUHSC_00530		elongation factor Tu   (data from MRSA252)
  SAOUHSC_00878		hypothetical protein   (data from MRSA252)
  SAOUHSC_01040		pyruvate dehydrogenase complex, E1 component subunit alpha   (data from MRSA252)
  SAOUHSC_01041		pyruvate dehydrogenase complex, E1 component subunit beta   (data from MRSA252)
  SAOUHSC_01042		branched-chain alpha-keto acid dehydrogenase subunit E2   (data from MRSA252)
  SAOUHSC_01490		DNA-binding protein HU   (data from MRSA252)
  SAOUHSC_01801		isocitrate dehydrogenase   (data from MRSA252)
  SAOUHSC_02441		alkaline shock protein 23   (data from MRSA252)
  SAOUHSC_02969		arginine deiminase   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: glmU > SAOUHSC_00472
  predicted SigA promoter  : glmU > S156 > SAOUHSC_00472 > S157 > SAOUHSC_00474

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser:   
  
  ⊟Multi-gene expression profiles

  Compare with:

  Conditions

  Select all flucRPMI-exp RPMI-exp-A2 vancRPMI-exp RPMI-exp-A1 TSB-exp-A TSB-exp-C ciproTSB-exp T/SmxTSB-exp clindTSB-exp lineTSB-exp eryTSB-exp TSB-exp-B3 flucTSB-exp TSB-exp-B2 vancTSB-exp TSB-exp-B1 RPMI/FCS-exp colist-exp intTHP1-2h intTHP1-6h intS9-6h intS9-2h TSB-t2-C TSB-t4-C flucTSB-t4 TSB-t2-B1 TSB-t4-B1 CDM-t2 CDM-t4 T/SmxTSB-t4 TSB-t4-B3 lineTSB-t4 TSB-t4-B2 vancTSB-t4 eryTSB-t4 clindTSB-t4 ciproTSB-t4 pMEM-t2-C1 pMEM-t4-C1 CO2-6h CO2-2h anaer-2h nonad50-1h CO2-1h nonad25-1h pMEM-exp-C2 pMEM-exp-C1 CDM-exp plasma-t4 vancRPMI-t4 flucRPMI-t4 RPMI-t4-A2 RPMI-t2-A1 plasma-exp

  
  
  Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications