PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Views

More

• Read
• Edit
• History
• Edit source

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS12400 [old locus tag: SA2160 ]
pan locus tag^?: SAUPAN005886000
symbol: SA_RS12400
pan gene symbol^?: —
• synonym:
• product: quinone oxidoreductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12400 [old locus tag: SA2160 ]
• symbol: SA_RS12400
• product: quinone oxidoreductase
• replicon: chromosome
• strand: +
• coordinates: 2427271..2428317
• length: 1047
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2427271..2428317) NCBI
• BioCyc: SA_RS12400 BioCyc
• MicrobesOnline: see SA2160

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGGCCGTATTTTCAAAAGAGAAAAAGAGAGGATGTATCGTTGTGATAGAAACATTTAAA
  GCGTTTGTAATTGATAAAGATGAGAGCGGTAAAGTGACACCAACTTTCAAACAATTATCG
  CCTACTGATTTACCTAAAGGAGATGTGCTGATTAAAGTACATTACTCTGGTATAAATTAT
  AAAGATGCTTTAGCGACTCAAGATCATAATGCAGTCGTAAAATCGTATCCTATGATTCCA
  GGAATAGATTTAGCTGGAACAATTGTTGAATCCGAAGCACCAGGCTTTGAAAAAGGAGAA
  CAAGTAATTGTAACGAGTTATGACCTAGGTGTTAGCCATTATGGCGGTTTTAGTGAATAT
  GCGCGTGTAAAATCAGAATGGATTATCAAGCTTCCTGATACTTTAACATTAGAAGAATCA
  ATGATATATGGCACAGCTGGTTATACTGCCGGTTTAGCAATTGAAAGACTTGAAAAAGTT
  GGAATGAATATTGAAGATGGTCCTGTACTCGTTCGCGGTGCTTCAGGTGGTGTCGGTACT
  TTAGCAGTACTCATGCTTAATGAACTTGGTTATAAAGTTATCGCAAGTACAGGTAAACAA
  GATGTTAGCGATCAATTACTTGAACTTGGTGCCAAAGAAGTTATCGATCGACTTCCTGTT
  GAAGATGATCATAAAAAGCCACTCGCATCATCAACTTGGCAAGCTTGTGTAGACCCTGTT
  GGTGGCGAAGGTATTAATTATGTTACAAAACGTTTAAATCATAGTGGATCAATTGCAGTT
  ATTGGTATGACTGCCGGCAATACTTATACTAATTCTGTATTCCCTCACATTTTAAGAGGC
  GTAAACATTTTAGGAATTGACTCAGTATTTACTGCTATGAAATTAAGACAGCGCGTTTGG
  CGTCGTCTCGCAAAAGATTTAAAGCCTGAAAATTTACATGAGATCAAGCAAGTTATTACA
  TTTGATGAACTTCCAGAACAACTTAACAAAGTAATTAATCATGAAAATAAAGGCCGCATT
  GTTATCGATTTCGGTGTAGATAAATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1047
  

⊟Protein

⊟General

• locus tag: SA_RS12400 [old locus tag: SA2160 ]
• symbol: SA_RS12400
• description: quinone oxidoreductase
• length: 348
• theoretical pI: 6.32425
• theoretical MW: 38098.5
• GRAVY: -0.112931

⊟Function

• ⊞TIGRFAM:
  Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 451.8)
  and 8 more
  Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 95.6)
  crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 58.8)
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 38.4)
  leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 35.3)
  Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 30.6)
  Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 26.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 25)
  6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 12.7)
• TheSEED: see SA2160
• ⊞PFAM:
  GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 59.3)
  and 4 more
  NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 45.7)
  ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 14.8)
  GroES (CL0296) ADH_N_2; N-terminal domain of oxidoreductase (PF16884; HMM-score: 13.8)
  NADP_Rossmann (CL0063) ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9279
  • Cytoplasmic Membrane Score: 0.0079
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0642
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006704
  • TAT(Tat/SPI): 0.000185
  • LIPO(Sec/SPII): 0.001224
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 487708286 NCBI
• RefSeq: WP_001792518 NCBI
• UniProt: see SA2160

⊟Protein sequence

• MAVFSKEKKRGCIVVIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGINYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGGFSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGASGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASSTWQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVFTAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVINHENKGRIVIDFGVDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications