PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_1609 [new locus tag: NWMN_RS09020 ]
pan locus tag^?: SAUPAN004367000
symbol: thiI
pan gene symbol^?: thiI
• synonym:
• product: thiamine biosynthesis protein ThiI

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1609 [new locus tag: NWMN_RS09020 ]
• symbol: thiI
• product: thiamine biosynthesis protein ThiI
• replicon: chromosome
• strand: -
• coordinates: 1786104..1787327
• length: 1224
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330945 NCBI
• RefSeq: YP_001332643 NCBI
• BioCyc:
• MicrobesOnline: 3707161 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAGTATGATCACTTGCTTGTTAGATACGGGGAGTTAACATTAAAGGGTTCAAATAGA
  AAGAAATTTGTAAATCAATTAAGAAATAATGTAAATAAGTCATTAAAAGGACTTGATGGG
  TTTGTCGTTAAAGGCAAACGAGATCGTATGTATATTGAACTTGAAGACCATGCAGATATA
  AATGAAATAACATATCGATTATCAAAAATTTTCGGTATTAAATCTATTAGTCCAGTATTA
  AAAGTAGAAAAAACAATAGAGGCAATAAGTGCAGCGGCAATTAAATTTGCGCAGCAATTT
  GAAGAAAACAGCACATTTAAAATTGATGTGAAGCGTGCCGATAAAAATTTCCCAATGGAT
  ACGTATGAATTACAGCGTGAATTGGGTGGTGCAGTATTGAAGCACTTCGACAATATTTCA
  GTGAATGTCAAACGTCCAGATCATGAAATTCGAGTGGAAGTTAGATTAGATGCAATTTAT
  ATGTATGAAGAAGTTGTTCCGGGTTCAGGTGGATTACCAGTTGGTACTGGTGGTAAGACG
  TTACTAATGCTTTCAGGCGGTATAGACTCACCAGTTGCTGGGATGGAAGTGATGAGACGT
  GGCGTAACAATTGAAGCGATTCATTTCCATAGTCCACCATTTACAAGTGATCAAGCAAAA
  GAAAAAGTTATTGAATTGACACGTATTTTAGCTGAACGTGTTGGACCAATTAAATTGCAT
  ATTGTACCATTTACAGAATTGCAAAAACAGGTAAATAAAGTTGTACATCCAAGATATACA
  ATGACTTCAACGAGACGTATGATGATGCGTGTTGCTGATAAATTAGTACATCAAATAGGG
  GCTTTAGCTATTGTAAATGGTGAAAACCTAGGGCAGGTAGCCAGTCAAACACTTCATAGC
  ATGTATGCAATTAATAATGTAACTTCTACTCCTGTATTACGTCCTTTATTAACTTACGAT
  AAAGAAGAAATTATTATTAAATCGAAAGAAATTGGTACATTTGAAACATCTATTCAACCA
  TTTGAAGATTGTTGTACAATTTTCACCCCTAAAAATCCAGTAACCGAACCAAACTTTGAT
  AAGGTAGTCCAATATGAAAGTGTCTTTGATTTTGAAGAGATGATTAATCGTGCTGTTGAA
  AATATTGAAACACTTGAAATAACTAGTGATTATAAAACTATTAAAGAACAGCAAACAAAC
  CAATTAATAAACGACTTTTTATAA
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  1224
  

⊟Protein

⊟General

• locus tag: NWMN_1609 [new locus tag: NWMN_RS09020 ]
• symbol: ThiI
• description: thiamine biosynthesis protein ThiI
• length: 407
• theoretical pI: 7.36521
• theoretical MW: 46209
• GRAVY: -0.279853

⊟Function

• ⊞reaction:
  EC 2.8.1.4?  ExPASy
  tRNA uracil 4-sulfurtransferase ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster
  [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP?
• ⊞TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 394)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 394)
  and 3 more
  Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 20.9)
  Hypothetical proteins Conserved putative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 20.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 13)
• TheSEED  :
  • tRNA S(4)U 4-thiouridine synthase (former ThiI)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  tRNA S(4)U 4-thiouridine synthase (former ThiI)
• ⊞PFAM:
  HUP (CL0039) ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 204.2)
  and 6 more
  no clan defined ThiI_fer; ThiI ferredoxin-like domain (PF22025; HMM-score: 89.8)
  THUMP (CL0747) THUMP; THUMP domain (PF02926; HMM-score: 66.3)
  HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 20.1)
  ATP_bind_3; PP-loop family (PF01171; HMM-score: 15.1)
  EpsM (CL0331) PilN_bio_d; PilN biogenesis protein dimerization domain (PF18222; HMM-score: 13)
  no clan defined Filo_VP35; Filoviridae VP35 (PF02097; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9889
  • Cytoplasmic Membrane Score: 0.0089
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0021
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004641
  • TAT(Tat/SPI): 0.000294
  • LIPO(Sec/SPII): 0.000906
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221821 NCBI
• RefSeq: YP_001332643 NCBI
• UniProt: A6QHP9 UniProt

⊟Protein sequence

• MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIYMYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTNQLINDFL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1608 < thiI < NWMN_1610

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications