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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01824
  • pan locus tag?: SAUPAN004367000
  • symbol: SAOUHSC_01824
  • pan gene symbol?: thiI
  • synonym:
  • product: thiamine biosynthesis protein ThiI

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01824
  • symbol: SAOUHSC_01824
  • product: thiamine biosynthesis protein ThiI
  • replicon: chromosome
  • strand: -
  • coordinates: 1729304..1730527
  • length: 1224
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAGTATGATCACTTGCTTGTTAGATACGGGGAGTTAACATTAAAGGGTTCAAATAGA
    AAGAAATTTGTAAATCAATTAAGAAATAATGTAAATAAGTCATTAAAAGGACTTGATGGG
    TTTGTCGTTAAAGGCAAACGAGATCGTATGTATATTGAACTTGAAGACCATGCAGATATA
    AATGAAATAACATATCGATTATCAAAAATTTTCGGTATTAAATCTATTAGTCCAGTATTA
    AAAGTAGAAAAAACAATAGAGGCAATAAGTGCAGCGGCAATTAAATTTGCGCAGCAATTT
    GAAGAAAACAGCACATTTAAAATTGATGTGAAGCGTGCCGATAAAAATTTCCCAATGGAT
    ACGTATGAATTACAGCGTGAATTGGGTGGTGCAGTATTGAAGCACTTCGACAATATTTCA
    GTGAATGTCAAACGTCCAGATCATGAAATTCGAGTGGAAGTTAGATTAGATGCAATTTAT
    ATGTATGAAGAAGTTGTTCCGGGTTCAGGTGGATTACCAGTTGGTACTGGTGGTAAGACG
    TTACTAATGCTTTCAGGCGGTATAGACTCACCAGTTGCTGGGATGGAAGTGATGAGACGT
    GGCGTAACAATTGAAGCGATTCATTTCCATAGTCCACCATTTACAAGTGATCAAGCAAAA
    GAAAAAGTTATTGAATTGACACGTATTTTAGCTGAACGTGTTGGACCAATTAAATTGCAT
    ATTGTACCATTTACAGAATTGCAAAAACAGGTAAATAAAGTTGTACATCCAAGATATACA
    ATGACTTCAACGAGACGTATGATGATGCGTGTTGCTGATAAATTAGTACATCAAATAGGG
    GCTTTAGCTATTGTAAATGGTGAAAACCTAGGGCAGGTAGCCAGTCAAACACTTCATAGC
    ATGTATGCAATTAATAATGTAACTTCTACTCCTGTATTACGTCCTTTATTAACTTACGAT
    AAAGAAGAAATTATTATTAAATCGAAAGAAATTGGTACATTTGAAACATCTATTCAACCA
    TTTGAAGATTGTTGTACAATTTTCACCCCTAAAAATCCAGTAACCGAACCAAACTTTGAT
    AAGGTAGTCCAATATGAAAGTGTCTTTGATTTTGAAGAGATGATTAATCGTGCTGTTGAA
    AATATTGAAACACTTGAAATAACTAGTGATTATAAAACTATTAAAGAACAGCAAACAAAC
    CAATTAATAAACGACTTTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1224

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01824
  • symbol: SAOUHSC_01824
  • description: thiamine biosynthesis protein ThiI
  • length: 407
  • theoretical pI: 7.36521
  • theoretical MW: 46209
  • GRAVY: -0.279853

⊟Function[edit | edit source]

  • reaction:
    EC 2.8.1.4?  ExPASy
    tRNA uracil 4-sulfurtransferase ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP?
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 394)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 394)
    and 3 more
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 20.9)
    Hypothetical proteins Conserved putative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 20.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 13)
  • TheSEED  :
    • tRNA S(4)U 4-thiouridine synthase (former ThiI)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  tRNA S(4)U 4-thiouridine synthase (former ThiI)
  • PFAM:
    HUP (CL0039) ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 204)
    and 3 more
    no clan defined THUMP; THUMP domain (PF02926; HMM-score: 66)
    HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 19.9)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004641
    • TAT(Tat/SPI): 0.000294
    • LIPO(Sec/SPII): 0.000906
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIYMYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTNQLINDFL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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