From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1764 [new locus tag: SACOL_RS09020 ]
  • pan locus tag?: SAUPAN004367000
  • symbol: thiI
  • pan gene symbol?: thiI
  • synonym:
  • product: thiamine biosynthesis protein ThiI

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1764 [new locus tag: SACOL_RS09020 ]
  • symbol: thiI
  • product: thiamine biosynthesis protein ThiI
  • replicon: chromosome
  • strand: -
  • coordinates: 1803263..1804486
  • length: 1224
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAGTATGATCACTTGCTTGTTAGATACGGGGAGTTAACATTAAAGGGTTCAAATAGA
    AAGAAATTTGTAAATCAATTAAGAAATAATGTAAATAAGTCATTAAAAGGACTTGATGGG
    TTTGTCGTTAAAGGCAAACGAGATCGTATGTATATTGAACTTGAAGACCATGCAGATATA
    AATGAAATAACATATCGATTATCAAAAATTTTCGGTATTAAATCTATTAGTCCAGTATTA
    AAAGTAGAAAAAACAATAGAGGCAATAAGTGCAGCGGCAATTAAATTTGCGCAGCAATTT
    GAAGAAAACAGCACATTTAAAATTGATGTGAAGCGTGCCGATAAAAATTTCCCAATGGAT
    ACGTATGAATTACAGCGTGAATTGGGTGGTGCAGTATTGAAGCACTTCGACAATATTTCA
    GTGAATGTCAAACGTCCAGATCATGAAATTCGAGTGGAAGTTAGATTAGATGCAATTTAT
    ATGTATGAAGAAGTTGTTCCGGGTTCAGGTGGATTACCAGTTGGTACTGGTGGTAAGACG
    TTACTAATGCTTTCAGGCGGTATAGACTCACCAGTTGCTGGGATGGAAGTGATGAGACGT
    GGCGTAACAATTGAAGCGATTCATTTCCATAGTCCACCATTTACAAGTGATCAAGCAAAA
    GAAAAAGTTATTGAATTGACACGTATTTTAGCTGAACGTGTTGGACCAATTAAATTGCAT
    ATTGTACCATTTACAGAATTGCAAAAACAGGTAAATAAAGTTGTACATCCAAGATATACA
    ATGACTTCAACGAGACGTATGATGATGCGTGTTGCTGATAAATTAGTACATCAAATAGGG
    GCTTTAGCTATTGTAAATGGTGAAAACCTAGGGCAGGTAGCCAGTCAAACACTTCATAGC
    ATGTATGCAATTAATAATGTAACTTCTACTCCTGTATTACGTCCTTTATTAACTTACGAT
    AAAGAAGAAATTATTATTAAATCGAAAGAAATTGGTACATTTGAAACATCTATTCAACCA
    TTTGAAGATTGTTGTACAATTTTCACCCCTAAAAATCCAGTAACCGAACCAAACTTTGAT
    AAGGTAGTCCAATATGAAAGTGTCTTTGATTTTGAAGAGATGATTAATCGTGCTGTTGAA
    AATATTGAAACACTTGAAATAACTAGTGATTATAAAACTATTAAAGAACAGCAAACAAAC
    CAATTAATAAACGACTTTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1224

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1764 [new locus tag: SACOL_RS09020 ]
  • symbol: ThiI
  • description: thiamine biosynthesis protein ThiI
  • length: 407
  • theoretical pI: 7.36521
  • theoretical MW: 46209
  • GRAVY: -0.279853

⊟Function[edit | edit source]

  • reaction:
    EC 2.8.1.4?  ExPASy
    tRNA uracil 4-sulfurtransferase ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP?
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 394)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 394)
    and 3 more
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 20.9)
    Hypothetical proteins Conserved putative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 20.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 13)
  • TheSEED  :
    • tRNA S(4)U 4-thiouridine synthase (former ThiI)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  tRNA S(4)U 4-thiouridine synthase (former ThiI)
  • PFAM:
    HUP (CL0039) ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 204)
    and 3 more
    no clan defined THUMP; THUMP domain (PF02926; HMM-score: 66)
    HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 19.9)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004641
    • TAT(Tat/SPI): 0.000294
    • LIPO(Sec/SPII): 0.000906
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIYMYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTNQLINDFL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 65 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 12.36 h [5]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL1764&oldid=98455"