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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS05565 [old locus tag: SA0982 ]
  • pan locus tag?: SAUPAN003367000
  • symbol: SA_RS05565
  • pan gene symbol?: srtB
  • synonym:
  • product: SrtB family sortase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS05565 [old locus tag: SA0982 ]
  • symbol: SA_RS05565
  • product: SrtB family sortase
  • replicon: chromosome
  • strand: +
  • coordinates: 1111733..1112467
  • length: 735
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1111733..1112467) NCBI
  • BioCyc: SA_RS05565 BioCyc
  • MicrobesOnline: see SA0982

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAGAATGAAGCGATTTTTAACTATTGTACAAATTTTATTGGTTGTAATTATTATCATT
    TTTGGTTACAAAATTGTTCAAACATATATTGAAGACAAGCAAGAACGCGCAAATTATGAG
    AAATTACAACAAAAATTTCAAATGCTGATGAGCAAACATCAAGAACATGTGAGACCACAA
    TTTGAATCACTTGAAAAAATAAATAAAGACATTGTTGGATGGATAAAATTATCAGGAACA
    TCATTAAATTATCCAGTACTACAAGGTAAGACAAATCACGATTATTTAAATTTAGATTTT
    GAGCGAGAACATCGACGTAAAGGTAGTATTTTTATGGATTTTAGAAATGAATTGAAGAAT
    TTAAATCATAATACTATTTTATACGGGCACCATGTCGGTGATAATACGATGTTTGATGTG
    TTAGAAGATTATTTAAAGCAATCGTTTTATGAAAAACACAAGATAATTGAATTTGACAAT
    AAATATGGTAAATATCAATTGCAAGTATTTAGTGCATATAAAACTACTACTAAAGATAAT
    TACATACGTACAGATTTTGAAAATGATCAAGATTATCAACAATTTTTAGATGAGACAAAA
    CGTAAATCTGTAATTAATTCAGATGTTAATGTAACGGTAAAAGATAAAATAATGACTTTA
    TCAACGTGCGAAGATGCATATAGTGAAACAACGAAAAGAATTGTTGTTGTCGCAAAAATA
    ATTAAGGTAAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    735

⊟Protein[edit | edit source]

Protein Data Bank: 1NG5

⊟General[edit | edit source]

  • locus tag: SA_RS05565 [old locus tag: SA0982 ]
  • symbol: SA_RS05565
  • description: SrtB family sortase
  • length: 244
  • theoretical pI: 9.23187
  • theoretical MW: 29135.2
  • GRAVY: -0.580328

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cell structure Cell envelope Other sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 304.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 304.1)
    and 2 more
    Cell structure Cell envelope Other sortase (TIGR01076; EC 3.4.22.-; HMM-score: 13.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking sortase (TIGR01076; EC 3.4.22.-; HMM-score: 13.1)
  • TheSEED: see SA0982
  • ⊞PFAM:
    no clan defined Sortase; Sortase domain (PF04203; HMM-score: 66.9)
    and 5 more
    Death (CL0041) Death_Lrrd1; Lrrd1 death domain (PF24978; HMM-score: 15.2)
    no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 14.1)
    TPR (CL0020) TPR_21; Tetratricopeptide repeat-like domain (PF09976; HMM-score: 13.4)
    no clan defined Arc_PepC_II; Archaeal Peptidase A24 C-terminus Type II (PF06847; HMM-score: 9.5)
    C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 7.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.8357
    • Cell wall & surface Score: 0.0074
    • Extracellular Score: 0.1567
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.309695
    • TAT(Tat/SPI): 0.000617
    • LIPO(Sec/SPII): 0.021892
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQKFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFEREHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDNKYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDKIMTLSTCEDAYSETTKRIVVVAKIIKVS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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